Mouse Ras- related protein Rab- 19, Rab19 ELISA KIT

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Ras-related protein Rab-19, RAB19,RAB19, member RAS oncogene family; RAB19B; GTP-binding protein RAB19B;

Search name

Mouse Ras-related protein Rab-19 ELISA KIT ,Mouse  RAB19 ELISA KIT ,Mouse RAB19 ELISA KIT ,Mouse  member RAS oncogene family ELISA KIT ,Mouse RAB19B ELISA KIT ,Mouse GTP-binding protein RAB19B ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse Ras-related protein Rab-19 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ras-related protein Rab-19, During the reaction, Ras-related protein Rab-19 in the sample or standard competes with a fixed amount of biotin-labeled Ras-related protein Rab-19 for sites on a pre-coated Monoclonal antibody specific to Ras-related protein Rab-19. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ras-related protein Rab-19 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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