Mouse Renalase, RNLS ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Renalase,Monoamine oxidase-C(MAO-C,mMAO-C), RNLS,renalase, FAD-dependent amine oxidase; RENALASE; C10orf59; MAO-C;
monoamine oxidase-C
Search name
Mouse Renalase ELISA Kit, Mouse RNLS ELISA Kit, Mouse Monoamine oxidase-C ELISA Kit, Mouse MAO-C ELISA Kit, Mouse
mMAO-C ELISA Kit, Mouse FAD-dependent amine oxidase ELISA Kit, Mouse RENALASE ELISA Kit, Mouse C10orf59 ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse renalase,Renalase concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Renalase. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Renalase and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Renalase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Renalase in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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