Mouse Retbindin, Rtbdn ELISA KIT

Product Name:Mouse Retbindin, Rtbdn ELISA KIT
Packing:96T

Catalog No.:ELI-21594m

Gene Name:Mouse Rtbdn

Detect Range:78.1-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Mouse Rtbdn

Alternative Name:Mouse Retbindin, Rtbdn
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Retbindin, Rtbdn ELISA KIT allows for the in vitro quantitative determination of Mouse Rtbdn concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Retbindin, Rtbdn ELISA KIT has been pre-coated with an Mouse Retbindin, Rtbdn antibody specific to Mouse Rtbdn .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rtbdn and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rtbdn , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rtbdn in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!