Mouse Retinol dehydrogenase 10, Rdh10 ELISA KIT
Product Name:Mouse Retinol dehydrogenase 10, Rdh10 ELISA KIT
Packing:96T
Catalog No.:ELI-30113m
Gene Name:Mouse Rdh10
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Rdh10
Alternative Name:Mouse Retinol dehydrogenase 10, Rdh10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Retinol dehydrogenase 10, Rdh10 ELISA KIT allows for the in vitro quantitative determination of Mouse Rdh10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Retinol dehydrogenase 10, Rdh10 ELISA KIT has been pre-coated with an Mouse Retinol dehydrogenase 10, Rdh10 antibody specific to Mouse Rdh10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rdh10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rdh10 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rdh10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!