Mouse Separin, ESPL1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ESPL1,Separin,separin cysteine protease; separin; ESP1; SEPA; caspase-like protein ESPL1; extra spindle pole bodies homolog 1; extra spindle poles like 1; extra spindle poles-like 1 protein; extra spindle pole bodies 1 separase
Search name
Mouse ESPL1 ELISA KIT ,Mouse Separin ELISA KIT ,Mouse separin cysteine protease ELISA KIT ,Mouse separin ELISA KIT ,Mouse ESP1 ELISA KIT ,Mouse SEPA ELISA KIT ,Mouse caspase-like protein ESPL1 ELISA KIT ,Mouse extra spindle pole bodies homolog 1 ELISA KIT ,Mouse extra spindle poles like 1 ELISA KIT ,Mouse extra spindle poles-like 1 protein ELISA KIT ,Mouse extra spindle pole bodies 1 separase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination ofMouse Separin concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Separin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forSeparin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Separin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Separin in the samples is then determined by comparing the O.D. of the samples to the standard curve.