Mouse soluble MHC class I polypeptide- related sequence A, SMICA ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
soluble MHC class I polypeptide-related sequence A(MIC-A), sMICA,sMIC-A; sPERB11.1; soluble HLA class I antigen; MHC class I chain-related protein A;
soluble stress inducible class I homolog; soluble MHC class I polypeptide-related sequence A

Search name
Mouse soluble MHC class I polypeptide-related sequence A ELISA Kit, Mouse sMIC-A ELISA Kit, Mouse sMICA ELISA Kit, Mouse sPERB11
ELISA Kit, Mouse soluble HLA class I antigen ELISA Kit, Mouse soluble MHC class I chain-related protein A ELISA Kit, Mouse soluble stress inducible
class I homolog ELISA Kit, Mouse SMICA ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse mhc class i polypeptide-related sequence a,MIC-A
concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MIC-A. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MIC-A and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain MIC-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MIC-A in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

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