Mouse Splicing factor U2AF 26 kDa subunit, U2af1l4 ELISA KIT

Product Name:Mouse Splicing factor U2AF 26 kDa subunit, U2af1l4 ELISA KIT
Packing:96T

Catalog No.:ELI-28392m

Gene Name:Mouse U2af1l4

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Mouse U2af1l4

Alternative Name:Mouse Splicing factor U2AF 26 kDa subunit, U2af1l4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Splicing factor U2AF 26 kDa subunit, U2af1l4 ELISA KIT allows for the in vitro quantitative determination of Mouse U2af1l4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Splicing factor U2AF 26 kDa subunit, U2af1l4 ELISA KIT has been pre-coated with an Mouse Splicing factor U2AF 26 kDa subunit, U2af1l4 antibody specific to Mouse U2af1l4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse U2af1l4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse U2af1l4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse U2af1l4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!