Mouse Squalene synthase, FDFT1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

FDFT1,squalene synthase,DGPT; ERG9; SQS; FPP:FPP farnesyltransferase; presqualene-di-diphosphate synthase; squalene synthetase; farnesyl-diphosphate farnesyltransferase 1

Search name

Mouse FDFT1 ELISA KIT ,Mouse squalene synthase ELISA KIT ,Mouse DGPT ELISA KIT ,Mouse ERG9 ELISA KIT ,Mouse SQS ELISA KIT ,Mouse FPP ELISA KIT ,Mouse FPP farnesyltransferase ELISA KIT ,Mouse presqualene-di-diphosphate synthase ELISA KIT ,Mouse squalene synthetase ELISA KIT ,Mouse farnesyl-diphosphate farnesyltransferase 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Squalene synthase, FDFT1 beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Squalene synthase, FDFT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mouse Squalene synthase, FDFT1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Squalene synthase, FDFT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse Squalene synthase, FDFT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.