Mouse Stem Cell Factor Receptor, SCFR ELISA kit

96 Tests

FOR RESEARCH USE ONLY

Alternative name

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Intended use

Mouse SCFR ELISA Kit t allows for the in vitro quantitative determination of mouse Stem Cell Factor Receptort,SCFR concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SCFR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SCFR and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SCFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SCFR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.15-10.0 ng/ml

PDF Manual download

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