Mouse Super Oxidase Dimutase 2 ELISA kit
Product Name:Mouse Super Oxidase Dimutase 2 ELISA kit
Packing:96T
Catalog No.:ELA-E2219m
Gene Name:Mouse SOD-2
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse SOD-2
Alternative Name:Mouse Super Oxidase Dimutase 2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Super Oxidase Dimutase 2 ELISA kit allows for the in vitro quantitative determination of Mouse SOD-2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Super Oxidase Dimutase 2 ELISA kit has been pre-coated with an Mouse Super Oxidase Dimutase 2 antibody specific to Mouse SOD-2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse SOD-2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse SOD-2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse SOD-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!