Mouse Suppressor of cytokine signaling 3, Socs3 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
SOCS3,ATOD4; CIS3; Cish3; SOCS-3; SSI-3; SSI3; STAT-induced STAT inhibitor 3; cytokine-inducible SH2 protein 3; suppressor of cytokine signaling 3, SOCS-3
Search name
Mouse SOCS3 ELISA KIT ,Mouse ATOD4 ELISA KIT ,Mouse CIS3 ELISA KIT ,Mouse Cish3 ELISA KIT ,Mouse SOCS-3 ELISA KIT ,Mouse SSI-3 ELISA KIT ,Mouse SSI3 ELISA KIT ,Mouse STAT-induced STAT inhibitor 3 ELISA KIT ,Mouse cytokine-inducible SH2 protein 3 ELISA KIT ,Mouse suppressor of cytokine signaling 3 ELISA KIT ,Mouse SOCS-3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse suppressor of cytokine signaling 3,SOCS-3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SOCS-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SOCS-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SOCS-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOCS-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.