Mouse Tacrolimus, FK506 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Mouse Tacrolimus ELISA Kit, Mouse fujimycin ELISA Kit, Mouse FK-506 ELISA Kit, Mouse Prograf ELISA Kit, Mouse Advagraf ELISA Kit, Mouse Protopic ELISA Kit
Intended use
Mouse TACROLIMUS ELISA kit allows for the in vitro quantitative determination of Mouse Tacrolimus concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Mouse TACROLIMUS ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TACROLIMUS, During the reaction, TACROLIMUS in the sample or standard competes with a fixed amount of biotin-labeled TACROLIMUS for sites on a pre-coated Monoclonal antibody specific to TACROLIMUS. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TACROLIMUS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse TACROLIMUS ELISA kit Range 0.15-10ng/ml
Mouse TACROLIMUS ELISA kit manual download
Mouse TACROLIMUS ELISA kit Citation
Ginseng extract reduces tacrolimus-induced oxidative stress by modulating autophagy in pancreatic beta cells
SW Lim, L Jin, K Luo, J Jin, CW Yang - Laboratory Investigation, 2017
Laboratory Investigation volume 97, pages 1271–1281 (2017)
doi:10.1038/labinvest.2017.75
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