Mouse Tesmin, MTL5 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Mtl5,CXCDC2; MTLT; TESMIN; CXC domain containing 2; testis-specific metallothionein-like protein; metallothionein-like 5, testis-specific
Search name
Mouse Mtl5 ELISA KIT ,Mouse CXCDC2 ELISA KIT ,Mouse MTLT ELISA KIT ,Mouse TESMIN ELISA KIT ,Mouse CXC domain containing 2 ELISA KIT ,Mouse testis-specific metallothionein-like protein ELISA KIT ,Mouse metallothionein-like 5 ELISA KIT ,Mouse testis-specific ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Tesmin, MTL5 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tesmin, During the reaction, Tesmin in the sample or standard competes with a fixed amount of biotin-labeled Tesmin for sites on a pre-coated Monoclonal antibody specific to Tesmin. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tesmin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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