Mouse Transcription factor HES- 1, Hes1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Transcription factor HES-1,RHL,Hairy and enhancer of split 1,Hairy-like protein,HES1,HES-1; HHL; HRY; bHLHb39; class B basic helix-loop-helix protein 39; hairy homolog; hairy-like protein; transcription factor HES-1; hairy and enhancer of split 1

Search name

Mouse Transcription factor HES-1 ELISA KIT ,Mouse RHL ELISA KIT ,Mouse Hairy and enhancer of split 1 ELISA KIT ,Mouse Hairy-like protein ELISA KIT ,Mouse HES1 ELISA KIT ,Mouse HES-1 ELISA KIT ,Mouse HHL ELISA KIT ,Mouse HRY ELISA KIT ,Mouse bHLHb39 ELISA KIT ,Mouse class B basic helix-loop-helix protein 39 ELISA KIT ,Mouse hairy homolog ELISA KIT ,Mouse hairy-like protein ELISA KIT ,Mouse transcription factor HES-1 ELISA KIT ,Mouse hairy and enhancer of split 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse transcription factor hes-1,Transcription factor HES-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription factor HES-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Transcription factor HES-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Transcription factor HES-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Transcription factor HES-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.