Mouse Tripartite motif- containing protein 65, Trim65 ELISA KIT

Product Name:Mouse Tripartite motif- containing protein 65, Trim65 ELISA KIT
Packing:96T

Catalog No.:ELI-16593m

Gene Name:Mouse Trim65

Detect Range:31.2-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Mouse Trim65

Alternative Name:Mouse Tripartite motif-containing protein 65, Trim65
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Tripartite motif- containing protein 65, Trim65 ELISA KIT allows for the in vitro quantitative determination of Mouse Trim65 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Tripartite motif- containing protein 65, Trim65 ELISA KIT has been pre-coated with an Mouse Tripartite motif-containing protein 65, Trim65 antibody specific to Mouse Trim65 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Trim65 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Trim65 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Trim65 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!