Mouse Ubiquitin- conjugating enzyme E2 S, Ube2s ELISA KIT
Product Name:Mouse Ubiquitin- conjugating enzyme E2 S, Ube2s ELISA KIT
Packing:96T
Catalog No.:ELI-22299m
Gene Name:Mouse Ube2s
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Ube2s
Alternative Name:Mouse Ubiquitin-conjugating enzyme E2 S, Ube2s
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ubiquitin- conjugating enzyme E2 S, Ube2s ELISA KIT allows for the in vitro quantitative determination of Mouse Ube2s concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ubiquitin- conjugating enzyme E2 S, Ube2s ELISA KIT has been pre-coated with an Mouse Ubiquitin-conjugating enzyme E2 S, Ube2s antibody specific to Mouse Ube2s .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ube2s and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ube2s , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ube2s in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!