Mouse Ubiquitin- like protein ATG12, Atg12 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ATG12, Ubiquitin-like protein ATG12,Autophagy-related protein 12,APG12-like,ATG12 autophagy related 12 homolog; APG12; APG12L; FBR93; HAPG12; Apg12 autophagy yeast homolog; autophagy related 12
Search name
Mouse ATG12 ELISA KIT ,Mouse Ubiquitin-like protein ATG12 ELISA KIT ,Mouse Autophagy-related protein 12 ELISA KIT ,Mouse APG12-like ELISA KIT ,Mouse ATG12 autophagy related 12 homolog ELISA KIT ,Mouse APG12 ELISA KIT ,Mouse APG12L ELISA KIT ,Mouse FBR93 ELISA KIT ,Mouse HAPG12 ELISA KIT ,Mouse Apg12 autophagy yeast homolog ELISA KIT ,Mouse autophagy related 12 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse ubiquitin-like protein atg12,Ubiquitin-like protein ATG12 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin-like protein ATG12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ubiquitin-like protein ATG12 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin-like protein ATG12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ubiquitin-like protein ATG12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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