Mouse Ubiquitin- like protein ATG12, Atg12 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ATG12, Ubiquitin-like protein ATG12,Autophagy-related protein 12,APG12-like,ATG12 autophagy related 12 homolog; APG12; APG12L; FBR93; HAPG12; Apg12 autophagy yeast homolog; autophagy related 12

Search name

Mouse ATG12 ELISA KIT ,Mouse  Ubiquitin-like protein ATG12 ELISA KIT ,Mouse Autophagy-related protein 12 ELISA KIT ,Mouse APG12-like ELISA KIT ,Mouse ATG12 autophagy related 12 homolog ELISA KIT ,Mouse APG12 ELISA KIT ,Mouse APG12L ELISA KIT ,Mouse FBR93 ELISA KIT ,Mouse HAPG12 ELISA KIT ,Mouse Apg12 autophagy yeast homolog ELISA KIT ,Mouse autophagy related 12 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse ubiquitin-like protein atg12,Ubiquitin-like protein ATG12 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin-like protein ATG12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ubiquitin-like protein ATG12 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin-like protein ATG12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ubiquitin-like protein ATG12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PDF manual download