Mouse Ubiquitin- protein ligase E3C, Ube3c ELISA KIT

Product Name:Mouse Ubiquitin- protein ligase E3C, Ube3c ELISA KIT
Packing:96T

Catalog No.:ELI-44764m

Gene Name:Mouse Ube3c

Detect Range:78.1-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Mouse Ube3c

Alternative Name:Mouse Ubiquitin-protein ligase E3C, Ube3c
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ubiquitin- protein ligase E3C, Ube3c ELISA KIT allows for the in vitro quantitative determination of Mouse Ube3c concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Ubiquitin- protein ligase E3C, Ube3c ELISA KIT has been pre-coated with an Mouse Ubiquitin-protein ligase E3C, Ube3c antibody specific to Mouse Ube3c .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ube3c and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ube3c , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ube3c in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!