Mouse Vitamin A ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Vitamin A,

Search name
Mouse Vitamin A ELISA Kit,Vitamin A ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of general vitamin a,Vitamin A concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Vitamin A, During the reaction, Vitamin A in the sample or standard competes with a fixed amount
of biotin-labeled Vitamin A for sites on a pre-coated Monoclonal antibody specific to Vitamin A. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Vitamin A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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