Mouse Zona pellucida- binding protein 1, Zpbp ELISA KIT
Product Name:Mouse Zona pellucida- binding protein 1, Zpbp ELISA KIT
Packing:96T
Catalog No.:ELI-51186m
Gene Name:Mouse Zpbp
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Zpbp
Alternative Name:Mouse Zona pellucida-binding protein 1, Zpbp
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Zona pellucida- binding protein 1, Zpbp ELISA KIT allows for the in vitro quantitative determination of Mouse Zpbp concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Zona pellucida- binding protein 1, Zpbp ELISA KIT has been pre-coated with an Mouse Zona pellucida-binding protein 1, Zpbp antibody specific to Mouse Zpbp .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Zpbp and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Zpbp , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Zpbp in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!