Mouse MIP-1A ELISA KIT

Product Name:Mouse MIP-1A ELISA KIT

Product Name:Mouse MIP-1A ELISA KIT

Gene Name:Mouse MIP-1A

Detect Range:78-5000pg/ml

Sensitivity:39pg/ml

Target Protein Name:Mouse MIP-1A

Alternative Name:Mouse MIP-1A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse MIP-1A ELISA KIT allows for the in vitro quantitative determination of Mouse MIP-1A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse MIP-1A ELISA KIT has been pre-coated with an Mouse MIP-1A antibody specific to Mouse MIP-1A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse MIP-1A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse MIP-1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse MIP-1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!