General Noradrenaline, NE ELISA Kit 
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Noradrenaline,Norepinephrine,; norepi,NE,NA,NAd,norad

Search name
General Noradrenaline ELISA Kit, General Norepinephrine ELISA Kit,General norepi ELISA Kit,General NE ELISA Kit,General NA
ELISA Kit, General NAd ELISA Kit, General norad ELISA Kit, Noradrenaline ELISA Kit, Norepinephrine ELISA Kit, norepi ELISA
Kit, NE ELISA Kit, NA ELISA Kit, NAd ELISA Kit, norad ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of general noradrenaline,Noradrenaline concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Noradrenaline, During the reaction, Noradrenaline in the sample or standard competes with a fixed
amount of biotin-labeled Noradrenaline for sites on a pre-coated Monoclonal antibody specific to Noradrenaline. Excess conjugate and
unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Noradrenaline in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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