Monkey Insulin, INS ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Insulin,INS, IDDM2; ILPR; IRDN; MODY10; preproinsulin; proinsulin

Search name
Monkey Insulin ELISA Kit, Monkey INS ELISA Kit, Monkey IDDM2 ELISA Kit, Monkey ILPR ELISA Kit, Monkey MODY10 ELISA Kit,
Monkey preproinsulin ELISA Kit, Monkey proinsulin ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of monkey Insulin concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Insulin, During the reaction, Insulin in the sample or standard competes with a fixed amount of
biotin-labeled Insulin for sites on a pre-coated Monoclonal antibody specific to Insulin. Excess conjugate and unbound sample or standard
are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Insulin in the
samples is then determined by comparing the O.D. of the samples to the standard curve

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