GW1-PercevalHR
Catalog No. PVT10538
Packing 2ug
GW1-PercevalHR Information
Function Other source plasmids
Promoter: hCMV IE4 promoter
Replicon: pUC ori
Terminator: SV40 poly (A) signal
Plasmid classification: gene library plasmid; other gene plasmids; other source plasmids.
Plasmid size: 7010bp
Prokaryotic resistance: ampicillin Amp (100 g/ml)
Screening marker: neomycin Neo/G418
Clone: DH5a Escherichia coli
Culture conditions: 37 C, aerobic, LB
Expression host: mammalian cells such as 293T
Culture conditions: 37 C, 5%CO2
Induction mode: no need to induce
GW1-PercevalHR Description
GW1-PercevalHR drives PercevalHR gene expression through hCMV IE4 promoter.
GW1-PercevalHR Sequence
LOCUS Exported 7010 bp ds-DNA circular SYN 14-Aug-2017
KEYWORDS GW1-PercevalHR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7010)
TITLE Direct Submission
JOURNAL Exported 2017-08-14
FEATURES Location/Qualifiers
source 1..7010
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 189..1928
/codon_start=1
/note="PercevalHR"
/translation="MKKVESIIRPEKLEIVKKALSDAGYVGMTVSEVKGSGVQGGIFER
YRGRVYSAGYNSDNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVL
LPDNHYLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFT
GVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGL
QCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKG
IDFKEDGNILGHKLEYNGTIVDLIPKVKIELVVKEEDVDNVIDIICENARTGNPGDGKI
FVIPVERVVRVRTKEEGASGGGSGGGGASGMKKVEAIIRPEKLEIVKKALSDAGYVGMT
VSEVKGRGAGGGDLIPKVKIELVVKEEDVDNVIDIICENARTGNPGDGKIFVIPVERIV
RVRTKEEGASGGGGGSGGASGMKKVEAIIRPEKLEIVKKALNDDGYVGMTVSEVKGRGA
GGGDLIPKVKIELVVKEEDVDNIIDIICENARTGNPGDGKIFVIPVERVVRPRTKEEGK
EAL"
polyA_signal 2050..2184
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2610..3198)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3369..4229)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
misc_feature 5448..7010
/note="hCMV IE4 promoter"
enhancer 5448..5827
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 5828..6031
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 6032..7010
/note="CMV intron"
ORIGIN
1 aagcttcccg gccacagcaa tcgatccatg acacaaccag tgacaagcag tcccccagtg
61 aaaccgcatc cgcatccgag tccgataccg ataaagattc tgaatcggag tgagtgccgc
121 gtccgagagc gttccctgtc cacgtccacc atcggcggag caggtgtgcc tgaggcctct
181 agaccaccat gaaaaaggtt gaatccatca tcaggcccga aaagctggag atcgttaaga
241 aggctctctc ggacgctgga tatgtgggta tgaccgtctc tgaagtcaaa ggctctggcg
301 tccagggcgg catcttcgag aggtaccgag gaagggtgta ctctgcaggc tacaacagcg
361 acaacgtcta catcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
421 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
481 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcttccag tccaagctga
541 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
601 ggatcactct cggcatggac gagctgtaca agggcggttc cggaggcatg gtgagcaagg
661 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
721 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
781 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
841 tgggctacgg tctccaatgc ttcgcccgct accccgacca catgaagcag cacgacttct
901 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
961 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
1021 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag cttgagtaca
1081 acggcaccat agtagatctg atccctaagg taaaaattga actagtggtg aaggaggagg
1141 acgtcgataa cgtgatagac attatttgcg aaaatgcccg cacaggtaac cccggcgacg
1201 gtaagatatt tgtgatccca gtggagcgag tggtcagggt gcgaaccaaa gaggagggag
1261 catctggtgg tggatccggt ggtggcggtg catctggtat gaaaaaggtt gaagctatta
1321 ttcgcccaga gaaactggag attgttaaga aggccctgtc cgacgccggc tatgttggca
1381 tgaccgtttc cgaggttaaa ggccgcggtg ccggcggtgg cgacctgatt cctaaggtga
1441 agatcgagct ggttgttaag gaggaagacg ttgataatgt tatcgacatt atctgcgaga
1501 acgcccgcac tggcaatcct ggtgacggca aaatcttcgt tataccggtc gagcgaattg
1561 tgcgagtacg aaccaaagaa gagggtgcct ccggcggtgg cggtggctcc ggtggcgcca
1621 gcggcatgaa gaaggttgag gccatcatcc gcccagagaa gctggaaatc gttaagaagg
1681 ccttaaacga cgacggctac gtcggcatga cagttagcga ggttaaaggt cgcggtgccg
1741 gtgggggtga tctgattcct aaggttaaaa tcgagctcgt tgttaaggag gaggacgttg
1801 acaacattat cgatatcatc tgcgaaaacg ctcgcaccgg caacccaggc gacggcaaga
1861 ttttcgtcat tcctgttgag agagtggtgc gtccccgtac gaaggaggag ggcaaggaag
1921 cactgtgact cgacgaattc attgatcata atcagccata ccacatttgt agaggtttta
1981 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
2041 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
2101 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
2161 aatgtatctt atcatgtctg gatccgtacc gagctcgcgt aatcatggtc atagctgttt
2221 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
2281 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
2341 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
2401 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
2461 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
2521 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
2581 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
2641 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
2701 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
2761 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
2821 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
English