NM9-SpCas9-NLS3 Plasmid
Catalog No.:
PVT50049
NM9-SpCas9-NLS3 PlasmidAlias:Plasmid#128177Gene length:
Host:
yeast
Use(s):
gene editingFragment Type:
CRISPR
Fragmented species:
Prokaryotic resistance:
Kan
Screening Markers:
Neo/G418
Promoter:
pPGK1
replicon:
pUC
Copy Number:
Competent cells:
DH5a
Temperature:
37Degrees
Back Bones:
Forward primer:
Reverse primer:
Induction method:
Sequence:
gcgctaatttttcaaacaaagaatctgagctgcatttttacagaacagaaatgcaacgcgagagcgctattttaccaacaaagaatctatacttcttttttgttctacaaaaatgcatcccgagagcgctatttttctaacaaagcatcttagattactttttttctcctttgtgcgctctataatgcagtctcttgataactttttgcactgtaggtccgttaaggttagaagaaggctactttggtgtctattttctcttccataaaaaaagcctgactccacttcccgcgtttactgattactagcgaagctgcgggtgcattttttcaagataaaggcatccccgattatattctataccgatgtggattgcgcatactttgtgaacagaaagtgatagcgttgatgattcttcattggtcagaaaattatgaacggtttcttctattttgtctctatatactacgtataggaaatgtttacattttcgtattgttttcgattcactctatgaatagttcttactacaatttttttgtctaaagagtaatactagagataaacataaaaaatgtagaggtcgagtttagatgcaagttcaaggagcgaaaggtggatgggtaggttatatagggatatagcacagagatatatagcaaagagatacttttgagcaatgtttgtggaagcgccgcggtgatcacaggcagcaacgctctgtcatcgttacaatcaacatgctaccctccgcgagatcatccgtgtttcaaacccggcagcttagttgccgttcttccgaatagcatcggtaacatgagcaaagtctgccgccttacaacggctctcccgctgacgccgtcccggactgatgggctgcctgtatcgagtggtgattttgtgccgagctgccggtcggggagctgttggctggctggtggcaggatatattgtggtgtaaacaaattgacgcttagacaacttaataacacattgcggacgtttttaatgtactgaattaacgccgaattaattcctaggccaccatgttgggcccggcgcgccgaattcgagctcggtacccaactgcagaactacgccctcgcgcatccgacttcggactcgaccggcgaaacgactgcgacctatttctactgcttgccttcgacgtccgggcgagtcgccgcttaccccgtgcgctctcctccccctctggcccctcaaactgcaactcctgaccgcggcgatgcgcgcagtaccccgtcaaaatgcaactctacgcgactttcggcttagaagtcgccaagctccgtgcttcgccgcctcgagctcttgcgttgcctgcagacgtcgtctatcacgaggcggagaagctcgagttgccggctttgccgagggcggtcaaggttgagtcggagactgtcaaggtagaagttcgagaagaggaggatgaggttaagggtgaggtgctgttagtgaagacggactcggttgtgcaggaggacactgaagcgggctcggaaggagtgcaggttgaggaaacgatgctggtcaagctggaggcgagcagttgattgcccatccttgcaactcacagctgcttcacgttctctgcgcctcgttccatgcactgcgtcgcacttgtcggcgggcgaagacacgaggagaggacaggctgtgtcctcgagcgagagtagatgggaaggttactggaagtcgtgctgttttctatcggcttgcacgcatctgctctggttcactcgctgtgaggcccagcttccatgctgctgcgatctgggagtgcaagcccgcggtcagcagccattgcccgcctacaatgtcttcccaaagccgcccttgctcgctcgctgcgtggagttcgacgttctcctcgctccgcaagcattggaatgaaccttgctctctagttccctcctccgtgacctcgtttcgtcctttagacggcacgatggaaggaagaaatctctgcggacaagcaaatctgctggctcgccttgtaggtcgcctaccggagcaagccttgtgccgccgggatgccaacgtcgttttttgacgtttgcaagacgtagaggacgcttcggacgacgaaacaagctgtgaggacatggaagtcgtgggaggaacggcgcagagcggcgccgcgggagcataaggcaagcgagatagtccagaaatcgcggcgccaagtacagtaatttattggagcaggcaccagaagcgggcagcagtatgcgcaggcttggggtcgacgagagacgactccctcatactcggttacctcgagcaatacaatcaatcgaagctgcgcgaatctcggcttgtaagggtcggaaaggaacctcggagatggccacgtcacatcaccaacttatcgatctcagccgacgtcgcagagagggcgagcgaagcggtgaaggagggaaacaatccctcgagagcatgatccgtctgaatctgcagcgcaggaagccgtcacacgcccgcctcgagcgcaggtcgggtccagccgggggacgaaacgcgcgagggctgatttcgtgagcgaaggaagccgcatcgacaagttcgcgtccctttgccctctttcccatcacccgctctcgctctacccgctcagaacaacaccagatcagtcacaatgggcaaggagaagacgcacgtctcgcgcccgcgcctcaactcgaacatggacgccgacctctacggctacaagtggtcccgcgacaacgtcggccagtcgggcgccacgatctaccgcctctacggcaagccggacgccccggagctcttcctcaagcacggcaagggctcggtcgccaacgacgtcacggacgagatggtccgcctcaactggctcacggagttcatgccgctcccgacgatcaagcacttcatccgcacgccggacgacgcctggctcctcacgacggccatcccgggcaagacggccttccaggtcctcgaggagtacccggactcgggcgagaacatcgtcgacgccctcgccgtcttcctccgccgcctccactcgatcccggtctgcaactgcccgttcaactcggaccgcgtcttccgcctcgcccaggcccagtcgcgcatgaacaacggcctcgtcgacgcctcggacttcgacgacgagcgcaacggctggccggtcgagcaggtctggaaggagatgcacaagctcctcccgttctcgccggactcggtcgtcacgcacggcgacttctcgctcgacaacctcatcttcgacgagggcaagctcatcggctgcatcgacgtcggccgcgtcggcatcgccgaccgctaccaggacctcgccatcctctggaactgcctcggcgagttctcgccgtcgctccagaagcgcctcttccagaagtacggcatcgacaacccggacatgaacaagctccagttccacctcatgctcgacgagttcttctagatcattccactcaacattcaggctcctctgcgcacgtaaagtgccaaaggcaataccctgctcggtggaatgccgccgggcttgtcgattttacgcacatatgcgcattcttgacttgaagcggaggagttcttcgttgcgggttacagtgttttaataaaagaatggtcaaatcaaactgctagatatacctgtcagacactctagttgttgacccctatactcttaatacatcagacagtacatgcatgttgcatgatgatgataatgtctgtttagattccaagtgtctactgctggcgtcgacctagggggatcctctagagtcgacctgcaggcatgcaagctttaagaggagtccaccatggtagatctactggattttggttttaggaattagaaattttattgatagaagtattttacaaatacaaatacatactaagggtttcttatatgctcaacacatgagcgaaaccctataggaaccctaattcccttatctgggaactactcacacattattatggagaaaactagtctactcgtacttgacgcgcttgcgggtgcccgcgtcgcccgcctcgcactcgccgaggcggcgcttgagttcgcggatctcgtcgccgccgagctgcgagaggtcgatgcgcgtctcgtagaggccggtgatcgactggtggatgagggtcgcgtcgaggacttccttggtcgaggtgtagcgcttgcggtcgatggtggtgtcgaagtacttgaacgcggcaggggcgccgaggttggtgagggtgaagaggtggatgatgttctccgcctgctcgcggatgggcttgtcgcggtgcttgttgtaggccgagaggaccttgtcgaggttcgcgtcggcgaggatgacgcgcttcgagaactcgctgatctgctcgatgatctcgtcgaggtagtgcttgtgctgctcgacgaagagctgcttctgctcgttgtcctcgggcgagcccttgagcttctcgtagtgcgacgcgaggtagaggaagttgacgtacttcgacgggagggcgagttcgttgcccttctggagttcgccggccgaggcgagcatgcgcttgcggccgttctccaactcgaagagcgagtacttggggagcttgatgatgaggtccttcttgacttccttgtagcccttcgcttcgaggaagtcgatcgggttcttctcgaacgacgagcgctccatgatggtgatgccgaggagttccttgaccgacttgagcttcttcgacttgcccttctcgaccttcgcgacgacgaggaccgagtaggcgaccgtcggcgagtcgaatccgccgtacttcttcgggtcccagtccttcttgcgggcgatgagcttgtccgagttgcgcttggggaggatcgactccttcgagaagccgccggtctggacctcggtcttcttgacgatgttgacctgcggcatcgagaggaccttgcggacggtggcgaagtcgcggcccttgtcccagacgatctcgcccgtctcgccgttggtctcgatgagggggcgcttgcggatctcgccgttggcgagggtgatctcggtcttgaagaagttcatgatgttcgagtagaagaagtacttcgcggtggccttgccgatctcctgctccgacttggcgatcatcttgcggacgtcgtagaccttgtagtcgccgtagacgaactccgattcgagcttcgggtacttcttgatgagcgcggtgccgacgaccgcgttgaggtaggcgtcgtgggcgtggtggtagttgttgatctcgcggaccttgtagaactggaagtccttgcggaagtccgagacgagcttcgacttgagggtgatgaccttgacctcgcggatgagcttgtcgttctcgtcgtacttggtgttcatgcgcgagtcgaggatctgcgcgacgtgcttggtgatctggcgggtctcgacgagctggcgcttgatgaagcccgccttgtcgagttccgagagtccgccgcgctccgccttcgtgaggttgtcgaacttgcgctgggtgatgagcttggcgttgaggagctggcgccagtagttcttcatcttcttgacgacttcctccgaggggacgttgtccgacttgccgcggttcttgtccgagcgggtgaggaccttgttgtcgatcgagtcgtccttgaggaacgactgcgggacgatgtggtccacgtcgtagtccgagaggcggttgatgtcgagttcctggtccacgtacatgtcgcggccgttctggaggtagtagaggtagagcttctcgttctggagctgggtgttctcgacggggtgctccttgaggatctgcgagccgagttccttgatgccttcctcgatgcgcttcatgcgctcgcgcgagttcttctggcccttctgggtggtctggttctcgcgggccatctcgatgacgatgttctcgggcttgtggcggcccatgaccttgacgagttcgtccacgaccttgacggtctggaggatgcccttcttgatcgcgggcgagccggcgaggttggcgatgtgctcgtggagcgagtcgccctggcccgagacctgggccttctggatgtcttccttgaaggtgagcgagtcgtcgtggatgagctgcatgaagttgcggttggcgaagccgtccgacttgaggaagtcgaggatggtcttgcccgactgcttgtcgcggatgccgttgatgagcttgcgcgagaggcggccccagcccgtgtatcggcggcgcttgagctgcttcatgaccttgtcgtcgaagaggtgggcgtaggtcttgaggcgctcctcgatcatctcgcggtcctcgaagagggtgagggtgaggacgatgtcttcgaggatgtcctcgttctcctcgttgtcgaggaagtccttgtccttgatgatcttgaggaggtcgtggtaggtgccgagcgacgcgttgaagcggtcctcgacgccgctgatctcgaccgagtcgaagcactcgatcttcttgaagtagtcttccttgagctgcttgacggtgaccttgcggttggtcttgaagaggaggtccacgatcgccttcttctgctcgcccgagaggaaggccggcttgcgcatgccctcggtgacgtacttgaccttggtgagttcgttgtagacggtgaagtactcgtagaggagcgagtgcttggggaggaccttctcgttcgggaggttcttgtcgaagttggtcatgcgctcgatgaacgactgggccgaggcgcccttgtccacgacttcctcgaagttccagggggtgatggtctcctccgacttgcgggtcatccacgcgaagcgcgagttgccgcgggcgagggggccgacgtagtacgggatgcggaaggtgaggatcttctcgatcttctcgcggttgtccttgaggaacgggtagaagtcttcctggcggcggaggatggcgtggagttcgccgaggtggatctggtgggtgatcgagccgttgtcgaaggtgcgctgcttgcggaggaggtcctcgcggttgagcttgacgaggagttcctcggtgccgtccatcttttcgaggatcggcttgatgaacttgtagaactcttcctgcgacgcgccgccgtcgatgtagcccgcgtagccgttcttcgactggtcgaagaagatttccttgtacttctcggggagctgctggcggacgagcgccttgaggagcgtgaggtcctggtggtgctcgtcgtagcgcttgatcatcgacgccgagagcggggccttggtgatctcggtgttgacgcggaggatgtccgagaggaggatggcgtccgagaggttcttcgcggcgaggaagaggtccgcgtactggtcgccgatctgggcgaggaggttgtcgaggtcgtcgtcgtaggtgtccttcgagagctggagcttcgcgtcctcggcgaggtcgaagttcgacttgaagttgggggtgaggccgagcgagagggcgatgaggttgccgaagaggccgttcttcttctcgcccgggagctgggcgatgaggttttcgaggcggcgcgacttcgagaggcgggccgagaggatggccttggcgtccacgcccgaggcgttgatggggttctcctcgaagagctggttgtaggtctggacgagctggatgaagagcttgtccacgtccgagttgtccgggttgaggtcgccctcgatgaggaagtggccgcggaacttgatcatgtgcgcgagggcgaggtagatgaggcggaggtccgccttgtaggtcgagtccacgagcttcttgcggaggtggtagatggtggggtacttctcgtggtaggcgacctcgtccacgatgttgccgaagatcgggtggcgctcgtgcttcttgtcttcctcgacgaggaacgactcttcgaggcggtggaagaacgagtcgtccaccttcgccatctcgttcgagaagatttcctggaggtagcagatgcggttcttgcggcgggtgtatcggcggcgggccgtgcgcttgaggcgggtggcctccgccgtctcgcccgagtcgaagaggagggcgccgatgaggttcttcttgatcgagtggcggtcggtgttgccgaggaccttgaacttcttcgacgggaccttgtactcgtcggtgatgacggcccagccgaccgagttggtgccgatgtcgaggccgatcgagtacttcttgtccatgtgggagggtgggagttggacgggtgagttgctgtgaacctgcagagtagtaagcgagtgactgtagctgtaccagagcgagtgcgagtgagaagtgcaagttcaacgatgcggccgcacccgacgagcgagtgacgagcagggtcgacctcgctcacccgcttgcactcgtctggcgcattcgctgacaggctagacagactcacacaggcttgttctcccagggccaagtcgagcggcgcactccgagcgggtcaggcgacgaccacctcctccgcttggactcgccagggaagagtcgaccgactcgagggatacaagggagtgggacatgatacgagcaacagagtgcactgcagggatggacaacacaggcgcagggatccgcccgccgtcgctcacccagcccctcgtctcgctcgccagcatctcgacggagcactttcggccctcccctctcacgctcgactggctgtactcgcttccattgccgccaggatgtcccgtccaacctttcccacagacgagcgaggactgggccctggtcgagcagtaccgcctgctctgccaggggttgacggtcatggcggagtacgagcgggagcgggcgaggcgccagcagcgcggggaggatccggatgcgcagccgtgggatcctaggagtgcgtcgcagcgttctgcctcttctgccgcgcttgcttacagccacttgcctcgacagcctcggacgacccggccgtcgtcttcgccgccgaatgcctcttcaacgggccagactgggtcgagtcggaggaccacgcgtcgtcgccgtccgagcacgacgacgccgcctacgaggtgatcgactcgccctcgcaagcggccgacgcagccttcctgggcgtcctctccgccgtccagcaacaacaacagcggaacgcctcttcctcgtcctcgacatccttcacgacctcgatccccctctccctctcggacttgacagccctctcctccgccctccccctctcgtcctcctcctctcaaccgcctcaaacccacctctcaccctcccaaatccccgcagacggcatcctctccgactcggctctctcacccgcctccctcccaagcttgatgagcgccatcgccgagttggagcagtgcgtcgcgcgattgagtttggaggcgagcgaggcgaggaggttgcagaggcggatgaggggggccgtggtcggtgcttcggcggcgaatgtgggggcggtgggtgatggagaagcagaggcggcgaagaagaagggcaagaacaggaagaagaagaaaaaggtcgccccagcccctcctcctcctccccctctccctccggctgtccctgcgccacccgcgccgaaacagcgcgaaggagcaggaggagacgactcggatttgttgaacgcgctgcactcggtttcgatgctggatcgcagggcggacgagtaccgcgagcgattgagggttctcaaggtccgtctggcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattaaactatcagtgtttgacaggatatattggcgggtaaacctaagagaaaagagcgtttattagaataatcggatatttaaaaggg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//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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