ORFtag_TetR-SD_Frame1 plasmid

PVT100721

ORFtag_TetR-SD_Frame1 plasmid Information

Plasmid name: ORFtag_TetR-SD_Frame1
Alias: Plasmid #220981
Gene lenght: 1586bp
Host: Mammalian cells,Reverse Transcription Virus
Use(s): Tetracycline induction,Protein Expression
Fragment type: ORF
Species:
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: PGK
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: IRES2-F1
Reverse primer:
Copy number: High
Induction: Tetracycline induction

ORFtag_TetR-SD_Frame1 plasmid Description

ORFtag_TetR-SD_Frame1 This is a plasmid suitable for tetracycline induction and protein expression in mammalian cells, and it can also be used for retrovirus packaging.

ORFtag_TetR-SD_Frame1 plasmid Sequence

1 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 61 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 121 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta 181 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 241 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 301 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 361 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 421 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 481 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 541 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 601 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 661 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 721 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 781 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 841 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 901 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 961 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 1021 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 1081 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 1141 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 1201 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 1261 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 1321 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 1381 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca 1441 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtaa 1501 gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag 1561 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag 1621 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg 1681 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact 1741 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg 1801 agcctgggga ctttccacac cctaactgac acacattcca cagccggatc tacactccgc 1861 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc 1921 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 1981 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ccagcttacc 2041 tcccggtggt gggtcggtgg tccctgggca ggggtctccc gatcccggac gagcccccaa 2101 atgaaagacc cccgctgacg ggtagtcaat cactcagagg agaccctccc aaggaacagc 2161 gagaccacaa gtcggatgca actgcaagag ggtttattgg atacacgggt acccgggcga 2221 ctcagtcaat cggaggactg gcgccccgag tgaggggttg tgctagcttg ccaaacctac 2281 aggtggggtc tttcattccc ccctttttct ggagactaaa taaaatcttt tattttatcg 2341 atggtcaagt tccttctggt tctggctctg ctcatcgtcc tgcaggaccg gacgtgactg 2401 gagttcagac gtgtgctctt ccgatctcat gttaactcga gaattctacc gggtagggga 2461 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg 2521 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc 2581 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc 2641 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag 2701 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg 2761 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg 2821 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc 2881 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc 2941 gggcctttcg gaattcgcca ccatgattga acaagatgga ttgcacgcag gttctccggc 3001 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 3061 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 3121 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 3181 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 3241 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 3301 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 3361 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 3421 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 3481 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 3541 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 3601 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 3661 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 3721 catcgccttc tatcgccttc ttgacgagtt cttctgacgc gtcccctctc cctccccccc 3781 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 3841 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 3901 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 3961 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 4021 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 4081 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 4141 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 4201 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt 4261 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 4321 aaaacacgat gataatatcg ccacaaccag atctgccacc atgcccaaaa agaagaggaa 4381 agtgatgtct agactggaca agagcaaagt cataaactct gctctggaat tactcaatga 4441 agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc 4501 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgg caatcgagat 4561 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct 4621 gcggaacaac gccaagtcat tccgctgtgc tctcctctca catcgcgacg gggctaaagt 4681 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt 4741 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca 4801 ctttacactg ggctgcgtat tggaggatca ggagcatcaa gtagcaaaag aggaaagaga 4861 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgacca 4921 tcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa 4981 acagctaaag tgcgaaagcg gcgggccgga tatcggaggc ggatcaggtg gcggtagcgt 5041 ccccgatcgt aaggctgcgg tctctcattg gcaacaggac gactataagg accacgacgg 5101 agactacaag gatcatgata ttgattacaa agacgatgac gataagcagg taagtaatgc 5161 tttttttttc tcgctctcat ttttcaaaaa cccacataaa aattgaggaa agggaagaat 5221 tgttttctcc ttccagcacc tcgtaatttg acccgactga tggttcccat tagtcacata 5281 aagctgtagt caagtacaga cgtcccgcgg cttcgatacc gtcacgaagc aacggatccg 5341 gccggcgcct agagaaggag tgagggctgg ataaagggag gatcgaggcg gggtcgaacg 5401 aggaggttca agggggagag acggggcgga tggaggaaga ggaggcggag gcttagggtg 5461 tacaaagggc ttgacccagg gaggggggtc aaaagccaag gcttcccagg tcacgatgta 5521 ggggacctgg tctgggtgtc catgcgggcc aggtgaaaag accttgatct taacctgggt 5581 gatgaggtct cggttaaagg tgccgtctcg cggccatccg acgttaaagg ttggccattc 5641 tgcagagcag aaggtaaccc aacgtctctt cttgacatct accgactggt tgtgagcgat 5701 ccgctcgaca tctttccagt gacctaaggt caaacttaag ggagtggtaa cagtctggcc 5761 ctaattttca gacaaataca gaaacacagt cagacagaga caacacagaa cgatgctgca 5821 gcgctgcagc agacaagacg cgcggcttcg gttccaaacc gaaagcaaaa attcagacgg 5881 aggcgggaac tgttttaggt tctcgtctcc taccagaacc acatatcctg acggggtcgg 5941 attccacatc gactcccttc ctcaggtcgg gccacaaaaa cggcccccaa agtccctggg 6001 acgtctccca gggttgcggc cgggtgttca gaactcgtca gttccaccac gggtccgcca 6061 gatacagagc tagttagcta actagtacag acgcaggcgc ataacatcaa acatagacac 6121 tagacaatcg gacagacaca gataagttgc tggccagctt acctcccggt ggtgggtcgg 6181 tggtccctgg gcaggggtct ccaaatcccg gacgagcccc caaatgaaag acccccgtcg 6241 tgggtagtca atcactcaga ggagaccctc ccaaggaaca gcgagaccac gattcggatg 6301 caaacagcaa gaggctttat tgggaatacg ggtacccggg cgacgcagtc tatcggaaga 6361 ctggcgcgcc gagtgagggg ttgtgggctc ttttattgag ctctgcttat atagacctcc 6421 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga 6481 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt 6541 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc 6601 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag 6661 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc 6721 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc 6781 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt 6841 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat 6901 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa 6961 taactagtca ataatcaatg tcaacatggc ggtaatgttg gacatgagcc aatataaatg 7021 tacatattat gatatggata caacgtatgc aatggccaag ctaattcttg aagacgaaag 7081 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 7141 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 7201 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 7261 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 7321 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 7381 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 7441 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 7501 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct 7561 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 7621 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???