ORFtag_TetR-SD_Frame3 plasmid

PVT100636

ORFtag_TetR-SD_Frame3 plasmid Information

Plasmid name: ORFtag_TetR-SD_Frame3
Alias: Plasmid #220983
Gene lenght: 1392bp
Host: Mammalian cells,Reverse Transcription Virus
Use(s): Tetracycline induction,Protein Expression
Fragment type: ORF
Species:
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: PGK
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: PGK-F2
Reverse primer:
Copy number: High
Induction: Tetracycline induction

ORFtag_TetR-SD_Frame3 plasmid Description

ORFtag_TetR-SD_Frame3 This is a plasmid suitable for tetracycline induction and protein expression in mammalian cells, and it can also be used for retrovirus packaging.

ORFtag_TetR-SD_Frame3 plasmid Sequence

1 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 61 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 121 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta 181 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 241 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 301 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 361 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 421 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 481 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 541 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 601 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 661 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 721 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 781 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 841 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 901 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 961 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 1021 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 1081 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 1141 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 1201 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 1261 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 1321 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 1381 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca 1441 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtaa 1501 gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag 1561 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag 1621 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg 1681 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact 1741 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg 1801 agcctgggga ctttccacac cctaactgac acacattcca cagccggatc tacactccgc 1861 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc 1921 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 1981 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ccagcttacc 2041 tcccggtggt gggtcggtgg tccctgggca ggggtctccc gatcccggac gagcccccaa 2101 atgaaagacc cccgctgacg ggtagtcaat cactcagagg agaccctccc aaggaacagc 2161 gagaccacaa gtcggatgca actgcaagag ggtttattgg atacacgggt acccgggcga 2221 ctcagtcaat cggaggactg gcgccccgag tgaggggttg tgctagcttg ccaaacctac 2281 aggtggggtc tttcattccc ccctttttct ggagactaaa taaaatcttt tattttatcg 2341 atggtcaagt tccttctggt tctggctctg ctcatcgtcc tgcaggaccg gacgtgactg 2401 gagttcagac gtgtgctctt ccgatctcat gttaactcga gaattctacc gggtagggga 2461 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg 2521 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc 2581 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc 2641 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag 2701 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg 2761 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg 2821 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc 2881 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc 2941 gggcctttcg gaattcgcca ccatgattga acaagatgga ttgcacgcag gttctccggc 3001 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 3061 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 3121 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 3181 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 3241 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 3301 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 3361 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 3421 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 3481 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 3541 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 3601 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 3661 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 3721 catcgccttc tatcgccttc ttgacgagtt cttctgacgc gtcccctctc cctccccccc 3781 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 3841 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 3901 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 3961 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 4021 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 4081 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 4141 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 4201 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt 4261 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 4321 aaaacacgat gataatatcg ccacaaccag atctgccacc atgcccaaaa agaagaggaa 4381 agtgatgtct agactggaca agagcaaagt cataaactct gctctggaat tactcaatga 4441 agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc 4501 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgg caatcgagat 4561 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct 4621 gcggaacaac gccaagtcat tccgctgtgc tctcctctca catcgcgacg gggctaaagt 4681 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt 4741 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca 4801 ctttacactg ggctgcgtat tggaggatca ggagcatcaa gtagcaaaag aggaaagaga 4861 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgacca 4921 tcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa 4981 acagctaaag tgcgaaagcg gcgggccgga tatcggaggc ggatcaggtg gcggtagcgt 5041 ccccgatcgt aaggctgcgg tctctcattg gcaacaggac gactataagg accacgacgg 5101 agactacaag gatcatgata ttgattacaa agacgatgac gataaggcca ggtaagtaat 5161 gctttttttt ttctcgctct catttttcaa aaacccacat aaaaattgag gaaagggaag 5221 aattgttttc tccttccagc acctcgtaat ttgacccgac tgatggttcc cattagtcac 5281 ataaagctgt agtcaagtac agacgtcccg cggcttcgat accgtcacga agcaacggat 5341 ccggccggcg cctagagaag gagtgagggc tggataaagg gaggatcgag gcggggtcga 5401 acgaggaggt tcaaggggga gagacggggc ggatggagga agaggaggcg gaggcttagg 5461 gtgtacaaag ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc aggtcacgat 5521 gtaggggacc tggtctgggt gtccatgcgg gccaggtgaa aagaccttga tcttaacctg 5581 ggtgatgagg tctcggttaa aggtgccgtc tcgcggccat ccgacgttaa aggttggcca 5641 ttctgcagag cagaaggtaa cccaacgtct cttcttgaca tctaccgact ggttgtgagc 5701 gatccgctcg acatctttcc agtgacctaa ggtcaaactt aagggagtgg taacagtctg 5761 gccctaattt tcagacaaat acagaaacac agtcagacag agacaacaca gaacgatgct 5821 gcagcgctgc agcagacaag acgcgcggct tcggttccaa accgaaagca aaaattcaga 5881 cggaggcggg aactgtttta ggttctcgtc tcctaccaga accacatatc ctgacggggt 5941 cggattccac atcgactccc ttcctcaggt cgggccacaa aaacggcccc caaagtccct 6001 gggacgtctc ccagggttgc ggccgggtgt tcagaactcg tcagttccac cacgggtccg 6061 ccagatacag agctagttag ctaactagta cagacgcagg cgcataacat caaacataga 6121 cactagacaa tcggacagac acagataagt tgctggccag cttacctccc ggtggtgggt 6181 cggtggtccc tgggcagggg tctccaaatc ccggacgagc ccccaaatga aagacccccg 6241 tcgtgggtag tcaatcactc agaggagacc ctcccaagga acagcgagac cacgattcgg 6301 atgcaaacag caagaggctt tattgggaat acgggtaccc gggcgacgca gtctatcgga 6361 agactggcgc gccgagtgag gggttgtggg ctcttttatt gagctctgct tatatagacc 6421 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt 6481 ggaaagtccc gttgattttg gtgccaaaca aactcccatt gacgtcaatg gggtggagac 6541 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca 6601 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata 6661 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg 6721 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact 6781 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta 6841 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt 6901 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt 6961 aataactagt caataatcaa tgtcaacatg gcggtaatgt tggacatgag ccaatataaa 7021 tgtacatatt atgatatgga tacaacgtat gcaatggcca agctaattct tgaagacgaa 7081 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 7141 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 7201 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 7261 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 7321 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 7381 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 7441 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 7501 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt 7561 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 7621 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 7681 tt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???