p15A-Ptet-dacA-KanR

PVT38991     2ug

p15A-Ptet-dacA-KanR Information

Promoter: Tet

Replicator: p15A

Clone strain: Dh5a

Culture condition: 37 ℃

Plasmid host: Escherichia coli

Plasmid use: protein expression

Clip type: ORF

Prokaryotic resistance: Kan

p15A-Ptet-dacA-KanR Sequence
GGAGTGTATACTGGCTTACTATGTTGGCACTGATGAGGGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCTGC
ACCGGTGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCACTGACTCGCTACGCTCGGTCGTTCGAC
TGCGGCGAGCGGAAATGGCTTACGAACGGGGCGGAGATTTCCTGGAAGATGCCAGGAAGATACTTAACAGGGAAGTGAGA
GGGCCGCGGCAAAGCCGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGTGGTGG
CGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCGTGCGCTCTCCTGTTCCTGCCTTTCG
GTTTACCGGTGTCATTCCGCTGTTATGGCCGCGTTTGTCTCATTCCACGCCTGACACTCAGTTCCGGGTAGGCAGTTCGC
TCCAAGCTGGACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAA
CCCGGAAAGACATGCAAAAGCACCACTGGCAGCAGCCACTGGTAATTGATTTAGAGGAGTTAGTCTTGAAGTCATGCGCC
GGTTAAGGCTAAACTGAAAGGACAAGTTTTGGTGACTGCGCTCCTCCAAGCCAGTTACCTCGGTTCAAAGAGTTGGTAGC
TCAGAGAACCTTCGAAAAACCGCCCTGCAAGGCGGTTTTTTCGTTTTCAGAGCAAGAGATTACGCGCAGACCAAAACGAT
CTCAAGAAGATCATCTTATTAATCAGATAAAATATTTCTAGATTTCAGTGCAATTTATCTCTTCAAATGTAGCACCTGAA
GTCAGCCCCATACGATATAAGTTGTAATTCTGCCTTTTTACGGTTCCTGGCCTTAAGACCCACTTTCACATTTAAGTTGT
TTTTCTAATCCGCATATGATCAATTCAAGGCCGAATAAGAAGGCTGGCTCTGCACCTTGGTGATCAAATAATTCGATAGC
TTGTCGTAATAATGGCGGCATACTATCAGTAGTAGGTGTTTCCCTTTCTTCTTTAGCGACTTGATGCTCTTGATCTTCCA
ATACGCAACCTAAAGTAAAATGCCCCACTGCGCTGAGTGCATATAATGCATTCTCTAGTGAAAAACCTTGTTGGCATAAA
AAGGCTAATTGATTTTCGAGAGTTTCATACTGTTTTTCTGTAGGCCGTGTACCTAAATGTACTTTTGCTCCATCGCGATG
ACTTAGTAAAGCACATCTAAAACTTTTAGCGTTATTACGTAAAAAATCTTGCCAGCTTTCCCCTTCTAAAGGGCAAAAGT
GAGTATGGTGCCTATCTAACATCTCAATGGCTAAGGCGTCGAGCAAAGCCCGCTTATTTTTTACATGCCAATACAATGTA
GGCTGCTCTACACCTAGCTTCTGGGCGAGTTTACGGGTTGTTAAACCTTCGATTCCGACCTCATTAAGCAGCTCTAATGC
GCTGTTAATCACTTTACTTTTATCTAAACGAGACATCATTTCCTAATTGTTGACACTCTATCATTGATAGAGTTATTTTA
CCACTCCCTATCAGTGATAGAGAATAGTTCGATTTGTTTAACTTTAAGAAGGAGAATGGACTTTTCCAACATGAGTATCC
TTCACTATTTAGCGAATATTGTAGACATTTTGGTGGTTTGGTTCGTAATTTACAAGGTTATCATGCTTATCCGCGGCACG
AAAGCCGTCCAGCTGTTGAAGGGGATTTTCATTATTATTGCCGTCAAGTTACTTAGCGGCTTCTTCGGATTGCAGACAGT
GGAATGGATTACTGATCAAATGCTGACTTGGGGTTTCTTAGCCATTATCATTATCTTTCAACCGGAATTGCGTCGTGCCC
TGGAGACTTTGGGGCGTGGCAATATCTTTACCCGCTATGGATCACGCATTGAGCGTGAACAACACCACCTTATTGAGTCT
ATTGAAAAGTCCACGCAATACATGGCGAAGCGTCGCATTGGAGCTTTGATCTCTGTGGCTCGTGATACAGGCATGGACGA
CTACATCGAGACTGGCATTCCGCTTAATGCGAAAATCTCATCGCAATTATTGATTAATATCTTCATCCCCAATACCCCTC
TTCACGATGGCGCAGTGATCATTAAAGGTAACGAGATCGCGAGCGCTGCCAGTTATCTGCCATTGTCCGACTCGCCGTTT
CTTTCTAAGGAGCTGGGAACTCGCCATCGTGCCGCATTAGGGATTTCCGAGGTGACAGATTCAATCACAATCGTTGTCAG
CGAGGAAACAGGTGGGATTTCCCTTACGAAGGGAGGCGAACTGTTCCGTGATGTATCCGAAGAAGAATTGCATAAGATTT
TGCTTAAAGAGCTGGTGACTGTCACAGCTAAAAAACCAAGCATTTTCTCCAAATGGAAAGGAGGTAAGTCCGAGTGAGAA
GATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATC
AAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAAGCCCAATCTGAATAATGTTACAACCAA
TTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATA
TTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGT
CTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCA
CCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATT
ACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGAT
CGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCA
CCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTTCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGG
AGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACAT
CATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAGCGATAGATTGTCGCA
CCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTCGA
CGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATA
TATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACGGGCCAGAGCTGCATCGCGCGTTTCGGTGATGACGGT
GAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCA
GGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACC
ATATGCGGTGTGAAATACCGCACAGAT
//

Caution:
1.  This product is FOR RESEARCH USE ONLY!
2.  The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.  Shipping temperature is 2-8℃.