pAAV-CMV-Hpgd-3×FLAG-ZsGreen1 plasmid

PVT98000

pAAV-CMV-Hpgd-3×FLAG-ZsGreen1 plasmid Information

Plasmid name: pAAV-CMV-Hpgd(mouse)-3×FLAG-ZsGreen1
Alias: NM_008278.2;15-PGDH
Gene lenght: 807bp
Host: Mammalian cells,Adeno associated virus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Middle
Induction:

pAAV-CMV-Hpgd-3×FLAG-ZsGreen1 plasmid Description

pAAV-CMV-Hpgd-3×FLAG-ZsGreen1 This is a plasmid suitable for protein expression in mammalian cells and can also be used for adeno-associated virus packaging.

pAAV-CMV-Hpgd-3×FLAG-ZsGreen1 plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtGACAT TGATTATTGA CTAGTTATTA 181 ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA 241 ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT 301 AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA 361 GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC 421 CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT 481 ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT 541 GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG 601 TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC 661 AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA 721 GGTCTATATA AGCAGAGCTg gtaccgccac catgcacgtg aacggcaaag tggctctggt 781 gaccggcgcg gcacagggca taggcaaagc cttcgctgag gctctgctgc tccatggcgc 841 caaggtagca ttggtggatt ggaatcttga ggcaggtgta aaatgtaaag ctgccctaga 901 tgagcagttc gaacctcaga agacactgtt cgtccagtgt gatgtggctg accagaaaca 961 actgagagat acttttagaa aagttgtaga ccatttcgga agattggata ttttggtcaa 1021 caatgcaggc gtgaacaatg agaaaaactg ggaacagact ctacaaatta atttggtttc 1081 tgttatcagt gggacctatc ttggtttgga ttacatgagt aagcaaaacg gaggtgaagg 1141 cggcatcatc atcaatatgt cttcattagc agggctcatg cctgttgcgc agcaacctgt 1201 ttattgtgct tcgaagcacg gcatcatcgg attcacacgc tcagcagcga tggctgctaa 1261 cctcatgaaa agcggtgtaa gactgaatgt catttgccca ggctttgtgg acacacccat 1321 ccttgaatcc attgaaaaag aagaaaatat gggccaatat atagaatata aggaccagat 1381 caaggctatg atgaaattct atggggtttt acacccatca accattgcca atggattaat 1441 aaatctcatt gaagatgatg ctctcaatgg tgccattatg aagatcacgg catcgaaagg 1501 aattcatttt caagattatg acatctctcc attactcgta aaagctccat taacctccGA 1561 CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA 1621 CAAGgctcta gagtcgacag agggcagagg aagtcttcta acatgcggtg acgtggagga 1681 gaatcccggc ccttccggaa ccatggccca gtccaagcac ggcctgacca aggagatgac 1741 catgaagtac cgcatggagg gctgcgtgga cggccacaag ttcgtgatca ccggcgaggg 1801 catcggctac cccttcaagg gcaagcaggc catcaacctg tgcgtggtgg agggcggccc 1861 cttgcccttc gccgaggaca tcttgtccgc cgccttcatg tacggcaacc gcgtgttcac 1921 cgagtacccc caggacatcg tcgactactt caagaactcc tgccccgccg gctacacctg 1981 ggaccgctcc ttcctgttcg aggacggcgc cgtgtgcatc tgcaacgccg acatcaccgt 2041 gagcgtggag gagaactgca tgtaccacga gtccaagttc tacggcgtga acttccccgc 2101 cgacggcccc gtgatgaaga agatgaccga caactgggag ccctcctgcg agaagatcat 2161 ccccgtgccc aagcagggca tcttgaaggg cgacgtgagc atgtacctgc tgctgaagga 2221 cggtggccgc ttgcgctgcc agttcgacac cgtgtacaag gccaagtccg tgccccgcaa 2281 gatgcccgac tggcacttca tccagcacaa gctgacccgc gaggaccgca gcgacgccaa 2341 gaaccagaag tggcacctga ccgagcacgc catcgcctcc ggctccgcct tgccctgaat 2401 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg 2461 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 2521 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 2581 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 2641 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 2701 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 2761 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat 2821 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 2881 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 2941 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 3001 cctccccgca tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc 3061 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct 3121 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg 3181 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt 3241 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc 3301 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat 3361 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag 3421 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg 3481 attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg 3541 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct 3601 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg 3661 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc 3721 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct 3781 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 3841 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 3901 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 3961 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct 4021 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 4081 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt 4141 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 4201 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg 4261 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 4321 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 4381 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat 4441 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg 4501 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 4561 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 4621 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 4681 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 4741 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 4801 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 4861 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 4921 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 4981 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 5041 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 5101 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 5161 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 5221 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 5281 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 5341 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 5401 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 5461 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 5521 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 5581 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 5641 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 5701 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 5761 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 5821 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 5881 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 5941 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 6001 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 6061 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 6121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 6181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 6241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???