pAAV-CMV-Pdk2-3×HA-Neo plasmid

PVT108709

pAAV-CMV-Pdk2-3×HA-Neo plasmid Information

Plasmid name: pAAV-CMV-Pdk2(mouse)-3×HA-Neo
Alias: NM_133667.2
Gene lenght: 1221
Host: Mammalian cells,Adeno associated virus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pAAV-CMV-Pdk2-3×HA-Neo plasmid Description

pAAV-CMV-Pdk2-3×HA-Neo This is a plasmid suitable for protein expression in mammalian cells and can also be used for adeno-associated virus packaging.

pAAV-CMV-Pdk2-3×HA-Neo plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt 361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa 661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt 841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata 901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac 961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc 1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata 1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta 1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt 1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg 1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat 1321 cgattgaatt cacGCCACCa tgcgctgggt ccgggcgctg ttgaagaatg cgtccctggc 1381 aggggcaccc aagtacatcg agcacttcag caagttctcc ccgtccccgt tgtccatgaa 1441 gcagtttcta gacttcggat ccagcaatgc ctgcgagaag acgtcattca ctttcctccg 1501 gcaggagctg cccgttcgcc tggccaacat catgaaagag atcaacctgc ttcctgaccg 1561 agtgctgggc accccatcgg tgcagctggt gcagagctgg tatgtccaga gtctgctgga 1621 catcatggaa ttcctggaca aagaccccga ggaccaccgg actctaagcc agttcacaga 1681 tgccctggtc accatccgga accggcacaa tgacgtggtg cccaccatgg cacagggagt 1741 gctggagtac aaggacacct atggagatga ccccgtctcc aaccagaaca tccagtactt 1801 cctggaccgc ttctacctca gccgcatctc catccgaatg ctaatcaatc aacacaccct 1861 catctttgat ggcagcacca acccagccca ccccaaacac attggcagca tcgatcccaa 1921 ctgcagcgtg tctgatgtgg tgaaagacgc ctatgacatg gctaagctcc tgtgtgacaa 1981 gtattacatg gcttcccctg acctggagat ccaggaagtc aatgccacca acgccaacca 2041 gcccattcac atggtctatg tcccttccca cctgtaccac atgctctttg aactctttaa 2101 gaatgccatg cgggccactg tggaaagcca cgagtccagc ctcacactcc cccccatcaa 2161 gattatggtg gccctcggtg aagaagatct gtccatcaaa atgagtgacc gaggcggggg 2221 tgtccccttg aggaagatcg agaggctctt cagctacatg tactccacag ctcccacacc 2281 ccagcctggc actgggggta ccccgctggc tggctttggg tacggactcc ccatttcccg 2341 cctctacgcc aagtacttcc agggggactt gcagcttttc tctatggagg gctttgggac 2401 agatgctgtc atctatctga aggccctgtc cacggactca gtggagcgcc tgcctgtcta 2461 caacaagtct gcctggcgcc actaccagac catccaggag gccggcgact ggtgtgtgcc 2521 cagcacagag cccaagaaca catcgacgta tcgggtcagc TACCCATACG ACGTACCTGA 2581 TTATGCAACT TATCCTTATG ATGTACCTGA TTATGCTAGC TATCCTTATG ATGTCCCAGA 2641 CTACGCATAG ggatcctcta gagtcgacct gcagaagctt gcctcgagca gcgctgctcg 2701 agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt 2761 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga 2821 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt 2881 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc 2941 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc 3001 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg 3061 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat 3121 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc 3181 tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg 3241 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag 3301 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc 3361 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc 3421 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 3481 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 3541 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 3601 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg 3661 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 3721 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 3781 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 3841 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat 3901 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc 3961 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag 4021 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg 4081 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 4141 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 4201 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 4261 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 4321 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 4381 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 4441 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 4501 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 4561 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 4621 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 4681 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 4741 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 4801 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 4861 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 4921 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 4981 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 5041 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 5101 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 5161 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 5221 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 5281 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 5341 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 5401 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 5461 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 5521 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 5581 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 5641 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 5701 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 5761 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 5821 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 5881 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 5941 tttgctggcc ttttgctcac atgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.