pAAV-cTNT-Tspan9-Cre plasmid

PVT96556

pAAV-cTNT-Tspan9-Cre plasmid Information

Plasmid name: pAAV-cTNT-Tspan9(mouse)-Cre
Alias: NM_001356301.2;6720474K14Rik; 9430079M16Rik; Tspan-9
Gene lenght:
Host: Mammalian cells,Adeno associated virus
Use(s): Gene editing
Fragment type: CRISPR
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Non
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: chimeric intron-F
Reverse primer:
Copy number: High
Induction:

pAAV-cTNT-Tspan9-Cre plasmid Description

pAAV-cTNT-Tspan9-Cre is a plasmid suitable for gene editing in mammalian cells, and can also be used for adeno-associated virus packaging.

pAAV-cTNT-Tspan9-Cre plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtgtgAC TAGTGCCCTT ACGGGCCCCC 181 CCTCGAGGTC GGGATAAAAG CAGTCTGGGC TTTCACATGA CAGCATCTGG GGCTGCGGCA 241 GAGGGTCGGG TCCGAAGCGC TGCCTTATCA GCGTCCCCAG CCCTGGGAGG TGACAGCTGG 301 CTGGCTTGTG TCAGCCCCTC GGGCACTCAC GTATCTCCGT CCGACGGGTT TAAAATAGCA 361 AAACTCTGAG GCCACACAAT AGCTTGGGCT TATATGGGCT CCTGTGGGGG AAGGGGGAGC 421 ACGGAGGGGG CCGGGGCCGC TGCTGCCAAA ATAGCAGCTC ACAAGTGTTG CATTCCTCTC 481 TGGGCGCCGG GCACATTCCT GCTGGCTCTG CCCGCCCCGG GGTGGGCGCC GGGGGGACCT 541 TAAAGCCTCT GCCCCCCAAG GAGCCCTTCC CAGACAGCCG CCGGCACCCA CCGCTCCGTG 601 GGACGATCCC CGAAGCTCTA GAGCTTTATT GCGGTAGTTT ATCACAGTTA AATTGCTAAC 661 GCAGTCAGTG CTTCTGACAC AACAGTCTCG AACTTAAGCT GCAGAAGTTG GTCGTGAGGC 721 ACTGGGCAGG TAAGTATCAA GGTTACAAGA CAGGTTTAAG GAGACCAATA GAAACTGGGC 781 TTGTCGAGAC AGAGAAGACT CTTGCGTTTC TGATAGGCAC CTATTGGTCT TACTGACATC 841 CACTTTGCCT TTCTCTCCAC AGGTGTGGTA CCatggccag gggctgtctc tgctgcttga 901 agtacacgat gttcctcttc aatttgatat tctggctctg tggctgtggg ctgcttggag 961 tgggcatctg gctctctgtg tcccaaggca acttcgccac cttctccccc agcttcccct 1021 cactgtccgc agccaacctc gtcatcgcca tcggcaccat cgtcatggtg acgggcttcc 1081 tgggctgcct gggggccatc aaggagaaca agtgtctgct tctcagcttc ttcatcgtgc 1141 tgttgatcat cctgctggca gagttgattc tgatcatcct cttcttcgtc tacatggaca 1201 aggtgaacga gaatgccaag caggacctga aagaggggct gctgctctac aacacagaga 1261 acaacgtggg gctcaagaac gcctggaaca tcatccaggc cgagatgcgg tgttgtgggg 1321 tcactgacta cacagactgg tatccggtgc tcggggagaa cacagttcct gacagatgct 1381 gcatggagaa ttcccagggc tgtgggcgca acagcaccac ccccttgtgg agaacgggct 1441 gctacgaaaa agtgaagctg tggtttgatg acaacaagca cgtgctaggc accgtgggca 1501 tgtgcatcct catcatgcag atcctgggaa tggccttttc catgacactg ttccagcaca 1561 tccaccggac aggtaaaaag tacgacgcct gagctagcgc caccatgccc aagaagaaga 1621 ggaaggtgtc caatttactg accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa 1681 cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag gcgttttctg 1741 agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga 1801 ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt ctatatcttc 1861 aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta aacatgcttc 1921 atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg gttatgcggc 1981 ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac 2041 gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc caggatatac 2101 gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc gaaattgcca 2161 ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc catattggca 2221 gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg ggggtaacta 2281 aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat aactacctgt 2341 tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag ctatcaactc 2401 gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct aaggatgact 2461 ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc gcgcgagata 2521 tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg accaatgtaa 2581 atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc 2641 tggaagatgg cgattagccg gttatcgata atcaacctct ggattacaaa atttgtgaaa 2701 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 2761 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 2821 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 2881 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 2941 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 3001 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 3061 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 3121 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 3181 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 3241 tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg ggtggcatcc 3301 ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag 3361 ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat 3421 tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc 3481 ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat 3541 ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca 3601 ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat 3661 attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa 3721 attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt 3781 aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct 3841 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt 3901 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg 3961 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt 4021 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 4081 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 4141 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 4201 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc 4261 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 4321 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat 4381 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 4441 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct 4501 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 4561 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 4621 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga 4681 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 4741 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 4801 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 4861 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 4921 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 4981 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 5041 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 5101 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 5161 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 5221 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 5281 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 5341 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 5401 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 5461 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 5521 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 5581 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 5641 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 5701 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 5761 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 5821 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 5881 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 5941 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 6001 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 6061 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 6121 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 6181 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 6241 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 6301 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 6361 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 6421 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 6481 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 6541 tgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???