pAAV-cTNT-ZsGreen1-Mcl1-shRNA2-miR30a plasmid

PVT109200

pAAV-cTNT-ZsGreen1-Mcl1-shRNA2-miR30a plasmid Information

Plasmid name: pAAV-cTNT-ZsGreen1-Mcl1(mouse)-shRNA2-miR30a
Alias: GCTCCGGAAACTGGACATTAAA
Gene lenght:
Host: Mammalian cells,Adeno associated virus
Use(s): Gene interference
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: cTNT
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: chimeric intron-F
Reverse primer:
Copy number: High
Induction:

pAAV-cTNT-ZsGreen1-Mcl1-shRNA2-miR30a plasmid Description

pAAV-cTNT-ZsGreen1-Mcl1-shRNA2-miR30a This plasmid is suitable for gene interference in mammalian cells and can also be used for adeno-associated virus packaging.

pAAV-cTNT-ZsGreen1-Mcl1-shRNA2-miR30a plasmid Sequence

1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGGTC GCGTCTAGTA CTAGTgggct ggaagctacc tttgacatca 181 tttcctctgc gaaCGGGCCC CCCCTCGAGG TCGGGATAAA AGCAGTCTGG GCTTTCACAT 241 GACAGCATCT GGGGCTGCGG CAGAGGGTCG GGTCCGAAGC GCTGCCTTAT CAGCGTCCCC 301 AGCCCTGGGA GGTGACAGCT GGCTGGCTTG TGTCAGCCCC TCGGGCACTC ACGTATCTCC 361 GTCCGACGGG TTTAAAATAG CAAAACTCTG AGGCCACACA ATAGCTTGGG CTTATATGGG 421 CTCCTGTGGG GGAAGGGGGA GCACGGAGGG GGCCGGGGCC GCTGCTGCCA AAATAGCAGC 481 TCACAAGTGT TGCATTCCTC TCTGGGCGCC GGGCACATTC CTGCTGGCTC TGCCCGCCCC 541 GGGGTGGGCG CCGGGGGGAC CTTAAAGCCT CTGCCCCCCA AGGAGCCCTT CCCAGACAGC 601 CGCCGGCACC CACCGCTCCG TGGGACGATC CCCGAAGCTC TAGAGCTTTA TTGCGGTAGT 661 TTATCACAGT TAAATTGCTA ACGCAGTCAG TGCTTCTGAC ACAACAGTCT CGAACTTAAG 721 CTGCAGAAGT TGGTCGTGAG GCACTGGGCA GGTAAGTATC AAGGTTACAA GACAGGTTTA 781 AGGAGACCAA TAGAAACTGG GCTTGTCGAG ACAGAGAAGA CTCTTGCGTT TCTGATAGGC 841 ACCTATTGGT CTTACTGACA TCCACTTTGC CTTTCTCTCC ACAGaaatgg aaagatGGTA 901 CCgccaccAT GGCCCAGTCC AAGCACGGCC TGACCAAGGA GATGACCATG AAGTACCGCA 961 TGGAGGGCTG CGTGGACGGC CACAAGTTCG TGATCACCGG CGAGGGCATC GGCTACCCCT 1021 TCAAGGGCAA GCAGGCCATC AACCTGTGCG TGGTGGAGGG CGGCCCCTTG CCCTTCGCCG 1081 AGGACATCTT GTCCGCCGCC TTCATGTACG GCAACCGCGT GTTCACCGAG TACCCCCAGG 1141 ACATCGTCGA CTACTTCAAG AACTCCTGCC CCGCCGGCTA CACCTGGGAC CGCTCCTTCC 1201 TGTTCGAGGA CGGCGCCGTG TGCATCTGCA ACGCCGACAT CACCGTGAGC GTGGAGGAGA 1261 ACTGCATGTA CCACGAGTCC AAGTTCTACG GCGTGAACTT CCCCGCCGAC GGCCCCGTGA 1321 TGAAGAAGAT GACCGACAAC TGGGAGCCCT CCTGCGAGAA GATCATCCCC GTGCCCAAGC 1381 AGGGCATCTT GAAGGGCGAC GTGAGCATGT ACCTGCTGCT GAAGGACGGT GGCCGCTTGC 1441 GCTGCCAGTT CGACACCGTG TACAAGGCCA AGTCCGTGCC CCGCAAGATG CCCGACTGGC 1501 ACTTCATCCA GCACAAGCTG ACCCGCGAGG ACCGCAGCGA CGCCAAGAAC CAGAAGTGGC 1561 ACCTGACCGA GCACGCCATC GCCTCCGGCT CCGCCTTGCC CTAAGGATCt cgatagtttg 1621 tttgaatgag gcttcagtac tttacagaat cgttgcctgc acatcttgga aacacttgct 1681 gggattactt cttcaggtta acccaacaga aggctcgaga aggtatattg ctgttgacag 1741 tgagcgACTC CGGAAACTGG ACATTAAATA GTGAAGCCAC AGATGTATTT AATGTCCAGT 1801 TTCCGGAGCt gcctactgcc tcggaattca aggggctact ttaggagcaa ttatcttgtt 1861 tactaaaact gaataccttg ctatctcttt gatacatttt tacaaagctg aattaaaatg 1921 gtataaatta aatcactttt ttcaattgga agactaatgc gGTCGACAAT CAACCTCTGG 1981 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT 2041 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT 2101 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA 2161 GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG 2221 CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG 2281 AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA 2341 ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA 2401 CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC 2461 TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC 2521 AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCGATA TCCGATCCAC CGGATCTAGA 2581 TAACTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC 2641 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT 2701 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA 2761 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGGT 2821 AACCACGTGC GGACCCAATG GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT 2881 CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG CGACCAAAGG TCGCCCGACG CCCGGGCTTT 2941 GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG CGCAGCTGCC TGCAGGGGCG CCTGATGCGG 3001 TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TACGTCAAAG CAACCATAGT 3061 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG 3121 CTACACTTGC CAGCGCCtTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA 3181 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA 3241 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTTGGG TGATGGTTCA CGTAGTGGGC 3301 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG 3361 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGGCTATTCT TTTGATTTAT 3421 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA 3481 ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTTATG GTGCACTCTC AGTACAATCT 3541 GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT 3601 GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT 3661 GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA 3721 TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA 3781 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA 3841 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA 3901 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC 3961 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG 4021 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC 4081 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT 4141 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT 4201 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT 4261 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA 4321 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC 4381 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA 4441 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG 4501 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC 4561 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT 4621 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT 4681 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG 4741 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG 4801 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA 4861 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA 4921 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA 4981 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA 5041 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT 5101 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT 5161 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT 5221 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT 5281 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA 5341 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG 5401 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC 5461 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA 5521 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA 5581 TGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.