pAAV-U6-Mir374b-shRNA1-ZsGreen1 plasmid

PVT92932

pAAV-U6-Mir374b-shRNA1-ZsGreen1 plasmid Information

Plasmid name: pAAV-U6-Mir374b(mouse)-shRNA1-ZsGreen1
Alias: CACTTAGCAGGTTGTATTATAT
Gene lenght:
Host: Mammalian cells,Adeno associated virus
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pAAV-U6-Mir374b-shRNA1-ZsGreen1 plasmid Description

pAAV-U6-Mir374b-shRNA1-ZsGreen1 is a plasmid suitable for use in mammalian cells, and can also be used for adeno-associated virus packaging.

pAAV-U6-Mir374b-shRNA1-ZsGreen1 plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg CGTTACATAA CTTACGGTAA 241 ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG 301 TTCCCATAGT AACGcCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT 361 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG 421 TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC 481 CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC 541 AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA 601 TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 661 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 721 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC 781 TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGCGGATT CGAATCCCGG CCGGGAACGG 841 TGCATTGGAA CGCGGATTCC CCGTGCCAAG AGTGACGTAA GTACCGCCTA TAGAGTCTAT 901 AGGCCCACAA AAAATGCTTT CTTCTTTTAA TATACTTTTT TGTTTATCTT ATTTCTAATA 961 CTTTCCCTAA TCTCTTTCTT TCAGGGCAAT AATGATACAA TGTATCATGC CTCTTTGCAC 1021 CATTCTAAAG AATAACAGTG ATAATTTCTG GGTTAAGGCA ATAGCAATAT TTCTGCATAT 1081 AAATATTTCT GCATATAAAT TGTAACTGAT GTAAGAGGTT TCATATTGCT AATAGCAGCT 1141 ACAATCCAGC TACCATTCTG CTTTTATTTT ATGGTTGGGA TAAGGCTGGA TTATTCTGAG 1201 TCCAAGCTAG GCCCTTTTGC TAATCATGTT CATACCTCTT ATCTTCCTCC CACAGCTCCT 1261 GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC AAAGAATTGG GATTCGAACA 1321 TCGATTGAAT TCCACCATGG CCCAGTCCAA GCACGGCCTG ACCAAGGAGA TGACCATGAA 1381 GTACCGCATG GAGGGCTGCG TGGACGGCCA CAAGTTCGTG ATCACCGGCG AGGGCATCGG 1441 CTACCCCTTC AAGGGCAAGC AGGCCATCAA CCTGTGCGTG GTGGAGGGCG GCCCCTTGCC 1501 CTTCGCCGAG GACATCTTGT CCGCCGCCTT CATGTACGGC AACCGCGTGT TCACCGAGTA 1561 CCCCCAGGAC ATCGTCGACT ACTTCAAGAA CTCCTGCCCC GCCGGCTACA CCTGGGACCG 1621 CTCCTTCCTG TTCGAGGACG GCGCCGTGTG CATCTGCAAC GCCGACATCA CCGTGAGCGT 1681 GGAGGAGAAC TGCATGTACC ACGAGTCCAA GTTCTACGGC GTGAACTTCC CCGCCGACGG 1741 CCCCGTGATG AAGAAGATGA CCGACAACTG GGAGCCCTCC TGCGAGAAGA TCATCCCCGT 1801 GCCCAAGCAG GGCATCTTGA AGGGCGACGT GAGCATGTAC CTGCTGCTGA AGGACGGTGG 1861 CCGCTTGCGC TGCCAGTTCG ACACCGTGTA CAAGGCCAAG TCCGTGCCCC GCAAGATGCC 1921 CGACTGGCAC TTCATCCAGC ACAAGCTGAC CCGCGAGGAC CGCAGCGACG CCAAGAACCA 1981 GAAGTGGCAC CTGACCGAGC ACGCCATCGC CTCCGGCTCC GCCTTGCCCT AAAGATCTAC 2041 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca 2101 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc 2161 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca 2221 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg 2281 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg 2341 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg 2401 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct 2461 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg 2521 attttgtagg taaccacgtg cggaccTCGA AATTCCCCAG TGGAAAGACG CGCAGGCAAA 2581 ACGCACCACG TGACGGAGCG TGACCGCGCG CCGAGCGCGC GCCAAGGTCG GGCAGGAAGA 2641 GGGCCTATTT CCCATGATTC CTTCATATTT GCATATACGA TACAAGGCTG TTAGAGAGAT 2701 AATTAGAATT AATTTGACTG TAAACACAAA GATATTAGTA CAAAATACGT GACGTAGAAA 2761 GTAATAATTT CTTGGGTAGT TTGCAGTTTT AAAATTATGT TTTAAAATGG ACTATCATAT 2821 GCTTACCGTA ACTTGAAAGT ATTTCGATTT CTTGGGTTTA TATATCTTGT GGAAAGGACG 2881 CGGGATCCGC ACTTAGCAGG TTGTATTATA Tttcaagaga ATATAATACA ACCTGCTAAG 2941 TGTTTTTAAG CTTGCGGccg caggaacccc tagtgatgga gttggccact ccctctctgc 3001 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc 3061 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt 3121 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc 3181 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 3241 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 3301 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 3361 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc 3421 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 3481 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg 3541 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 3601 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc 3661 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg 3721 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 3781 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg 3841 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt 3901 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 3961 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 4021 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 4081 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 4141 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 4201 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 4261 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 4321 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 4381 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 4441 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 4501 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 4561 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 4621 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 4681 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 4741 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 4801 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 4861 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 4921 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 4981 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 5041 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 5101 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 5161 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 5221 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 5281 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 5341 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 5401 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 5461 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 5521 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 5581 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 5641 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???