pAAV-U6-Npas4-shRNA2-ZsGreen1 plasmid

PVT104068

pAAV-U6-Npas4-shRNA2-ZsGreen1 plasmid Information

Plasmid name: pAAV-U6-Npas4(Rat)-shRNA2-ZsGreen1
Alias: GGTTGACCCTGATAATTTA
Gene lenght:
Host: Mammalian cells,Adeno associated virus
Use(s): Interference silence
Fragment type: shRNA
Species: Rat
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: U6
Promoter: pUC
Replicon:
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: Low
Induction:

pAAV-U6-Npas4-shRNA2-ZsGreen1 plasmid Description

pAAV-U6-Npas4-shRNA2-ZsGreen1 is a plasmid suitable for interference silencing in mammalian cells, and can also be used for adeno-associated virus packaging.

pAAV-U6-Npas4-shRNA2-ZsGreen1 plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg CGTTACATAA CTTACGGTAA 241 ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG 301 TTCCCATAGT AACGTCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT 361 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG 421 TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC 481 CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC 541 AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA 601 TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 661 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 721 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC 781 TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGCGGATT CGAATCCCGG CCGGGAACGG 841 TGCATTGGAA CGCGGATTCC CCGTGCCAAG AGTGACGTAA GTACCGCCTA TAGAGTCTAT 901 AGGCCCACAA AAAATGCTTT CTTCTTTTAA TATACTTTTT TGTTTATCTT ATTTCTAATA 961 CTTTCCCTAA TCTCTTTCTT TCAGGGCAAT AATGATACAA TGTATCATGC CTCTTTGCAC 1021 CATTCTAAAG AATAACAGTG ATAATTTCTG GGTTAAGGCA ATAGCAATAT TTCTGCATAT 1081 AAATATTTCT GCATATAAAT TGTAACTGAT GTAAGAGGTT TCATATTGCT AATAGCAGCT 1141 ACAATCCAGC TACCATTCTG CTTTTATTTT ATGGTTGGGA TAAGGCTGGA TTATTCTGAG 1201 TCCAAGCTAG GCCCTTTTGC TAATCATGTT CATACCTCTT ATCTTCCTCC CACAGCTCCT 1261 GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC AAAGAATTGG GATTCGAACA 1321 TCGATTGAAT TCCACCATGG CCCAGTCCAA GCACGGCCTG ACCAAGGAGA TGACCATGAA 1381 GTACCGCATG GAGGGCTGCG TGGACGGCCA CAAGTTCGTG ATCACCGGCG AGGGCATCGG 1441 CTACCCCTTC AAGGGCAAGC AGGCCATCAA CCTGTGCGTG GTGGAGGGCG GCCCCTTGCC 1501 CTTCGCCGAG GACATCTTGT CCGCCGCCTT CATGTACGGC AACCGCGTGT TCACCGAGTA 1561 CCCCCAGGAC ATCGTCGACT ACTTCAAGAA CTCCTGCCCC GCCGGCTACA CCTGGGACCG 1621 CTCCTTCCTG TTCGAGGACG GCGCCGTGTG CATCTGCAAC GCCGACATCA CCGTGAGCGT 1681 GGAGGAGAAC TGCATGTACC ACGAGTCCAA GTTCTACGGC GTGAACTTCC CCGCCGACGG 1741 CCCCGTGATG AAGAAGATGA CCGACAACTG GGAGCCCTCC TGCGAGAAGA TCATCCCCGT 1801 GCCCAAGCAG GGCATCTTGA AGGGCGACGT GAGCATGTAC CTGCTGCTGA AGGACGGTGG 1861 CCGCTTGCGC TGCCAGTTCG ACACCGTGTA CAAGGCCAAG TCCGTGCCCC GCAAGATGCC 1921 CGACTGGCAC TTCATCCAGC ACAAGCTGAC CCGCGAGGAC CGCAGCGACG CCAAGAACCA 1981 GAAGTGGCAC CTGACCGAGC ACGCCATCGC CTCCGGCTCC GCCTTGCCCT AAAGATCTAC 2041 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca 2101 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc 2161 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca 2221 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg 2281 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg 2341 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg 2401 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct 2461 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg 2521 attttgtagg taaccacgtg cggaccTCGA AATTCCCCAG TGGAAAGACG CGCAGGCAAA 2581 ACGCACCACG TGACGGAGCG TGACCGCGCG CCGAGCGCGC GCCAAGGTCG GGCAGGAAGA 2641 GGGCCTATTT CCCATGATTC CTTCATATTT GCATATACGA TACAAGGCTG TTAGAGAGAT 2701 AATTAGAATT AATTTGACTG TAAACACAAA GATATTAGTA CAAAATACGT GACGTAGAAA 2761 GTAATAATTT CTTGGGTAGT TTGCAGTTTT AAAATTATGT TTTAAAATGG ACTATCATAT 2821 GCTTACCGTA ACTTGAAAGT ATTTCGATTT CTTGGGTTTA TATATCTTGT GGAAAGGACG 2881 CGGGATCCgG GTTGACCCTG ATAATTTAtt caagagaTAA ATTATCAGGG TCAACCtttt 2941 ttctcgagac cttaggcaAA GCTTtgcgag ttgcacacgt gtcAAGGCTG CCAGCTaggt 3001 taatctcaat tcttggaagt tagAGCTCGC TTTTTAAGCT TGCGGccgca ggaaccccta 3061 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca 3121 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc 3181 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac 3241 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg 3301 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 3361 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 3421 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 3481 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 3541 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 3601 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 3661 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt 3721 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc 3781 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 3841 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 3901 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 3961 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 4021 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 4081 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 4141 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 4201 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 4261 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 4321 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 4381 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 4441 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 4501 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 4561 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 4621 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 4681 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 4741 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 4801 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 4861 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 4921 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 4981 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 5041 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 5101 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 5161 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 5221 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 5281 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 5341 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 5401 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 5461 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 5521 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 5581 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 5641 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 5701 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 5761 ccttttgctg gccttttgct cacatgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???