pAdtrack-CMV-METTL3 plasmid

PVT107952

pAdtrack-CMV-METTL3 plasmid Information

Plasmid name: pAdtrack-CMV-METTL3(rat)
Alias: NM_001024794.1;Mettl3
Gene lenght: 1743
Host: Mammalian cells,Adenovirus
Use(s): Gene expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pAdtrack-CMV-METTL3 plasmid Description

pAdtrack-CMV-METTL3 This is a plasmid suitable for protein expression in mammalian cells and can also be used for adenovirus packaging.

pAdtrack-CMV-METTL3 plasmid Sequence

1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgCATATGGA 61 TCCATGCATG TTAATTAACA TCATCAATAA TATACCTTAT TTTGGATTGA AGCCAATATG 121 ATAATGAGGG GGTGGAGTTT GTGACGTGGC GCGGGGCGTG GGAACGGGGC GGGTGACGTA 181 GGTTTTAGGG CGGAGTAACT TGTATGTGTT GGGAATTGTA GTTTTCTTAA AATGGGAAGT 241 GACGTAACGT GGGAAAACGG AAGTGACGAT TTGAGGAAGT TGTGGGTTTT TTGGCTTTCG 301 TTTCTGGGCG TAGGTTCGCG TGCGGTTTTC TGGGTGTTTT TTGTGGACTT TAACCGTTAC 361 GTCATTTTTT AGTCCTATAT ATACTCGCTC TGCACTTGGC CCTTTTTTAC ACTGTGACTG 421 ATTGAGCTGG TGCCGTGTCG AGTGGTGTTT TTTTAATAGG TTTTCTTTTT TACTGGTAAG 481 GCTGACTGTT ATGGCTGCCG CTGTGGAAGC GCTGTATGTT GTTCTGGAGC GGGAGGGTGC 541 TATTTTGCCT AGGCAGGAGG GTTTTTCAGG TGTTTATGTG TTTTTCTCTC CTATTAATTT 601 TGTTATACCT CCTATGGGGG CTGTAATGTT GTCTCTACGC CTGCGGGTAT GTATTCCCCC 661 GGGCTATTTC GGTCGCTTTT TAGCACTGAC CGATGTGAAT CAACCTGATG TGTTTACCGA 721 GTCTTACATT ATGACTCCGG ACATGACCGA GGAGCTGTCG GTGGTGCTTT TTAATCACGG 781 TGACCAGTTT TTTTACGGTC ACGCCGGCAT GGCCGTAGTC CGTCTTATGC TTATAAGGGT 841 TGTTTTTCCT GTTGTAAGAC AGGCTTCTAA TGTTTAAATG TTTTTTTGTT ATTTTATTTT 901 GTGTTTATGC AGAAACCCGC AGACATGTTT GAGAGAAAAA TGGTGTCTTT TTCTGTGGTG 961 GTTCCGGAGC TTACCTGCCT TTATCTGCAT GAGCATGACT ACGATGTGCT TTCTTTTTTG 1021 CGCGAGGCTT TGCCTGATTT TTTGAGCAGC ACCTTGCATT TTATATCGCC GCCCATGCAA 1081 CAAGCTATTG AATTCGTTTA AACTCCCTCT CAAGTCTGTA TACGGGGACA CGGACAGCCT 1141 TTTCGTCACC GAGCGTGGAC ACCGGCTCAT GGAAACCAGA GGTAAGAAAC GCATCAAAAA 1201 GCATGGGGGA AACCTGGTTT TTGACCCCGA ACGGCCAGAG CTCACCTGGC TCGTGGAATG 1261 CGAGACCGTC TGCGGGGCCT GCGGCGCGGA TGCCTACTCC CCGGAATCGG TATTTCTCGC 1321 GCCCAAGCTC TACGCCCTCA AAAGTCTGCA CTGCCCCTCG TGCGGCGCCT CCTCCAAGGG 1381 CAAGCTGCGC GCCAAGGGCC ACGCCGCGGA GGGGCTGGAC TATGACACCA TGGTCAAATG 1441 CTACCTGGCC GACGCGCAGG GCGAAGACCG GCAGCGCTTC AGCACCAGCA GGACCAGCCT 1501 CAAGCGCACC CTGGCCAGCG CGCAGCCCGG AGCGCACCCC TTCACCGTGA CCCAGACTAC 1561 GCTGACGAGG ACCCTGCGCC CGTGGAAAGA CATGACCCTG GCCCGTCTGG ACGAGCACCG 1621 ACTACTGCCG TACAGCGAAA GCCGCCCCAA CCCGCGAAAC GAGGAGATAT GCTGGATCGA 1681 GATGCCGTAG AGCAGGTGAC CGAGCTGTGG GACCGCCTGG AACTGCTTGG TCAAACGCTC 1741 AAAAGCATGC CTACGGCGGA CGGTCTCAAA CCGTTGAAAA ACTTTGCTTC CTTGCAAGAA 1801 CTGCTATCGC TGGGCGGCGA GCGCCTTCTG GCGGATTTGG TCAGGGAAAA CATGCGAGTC 1861 AGGGACATGC TTAACGAAGT GGCCCCCCTG CTCAGGGATG ACGGCAGCTG CAGCTCTCTT 1921 AACTACCAGT TGCAGCCGGT AATAGGTGTG ATTTACGGGC CCACCGGCTG CGGTAAGTCG 1981 CAGCTGCTCA GGAACCTGCT TTCTTCCCAG CTGATCTCCC CTACCCCGGA AACCGTTTTC 2041 TTCATCGCCC CGCAGGTAGA CATGATCCCC CCATCTGAAC TCAAAGCGTG GGAAATGCAA 2101 ATCTGTGAGG GTAACTACGC CCCTGGGCCG GATGGAACCA TTATACCGCA GTCTGGCACC 2161 CTCCGCCCGC GCTTTGTAAA AATGGCCTAT GACGATCTCA TCCTGGAACA CAACTATGAC 2221 GTTAGTGATC CCAGAAATAT CTTCGCCCAG GCCGCCGCCC GTGGGCCCAT TGCCATCATT 2281 ATGGACGAAT GCATGGAAAA TCTTGGAGGT CACAAGGGCG TCTCCAAGTT CTTCCACGCA 2341 TTTCCTTCTA AGCTACATGA CAAATTTCCC AAGTGCACCG GATACACTGT GCTGGTGGTT 2401 CTGCACAACA TGAATCCCCG GAGGGATATG GCTGGGAACA TAGCCAACCT AAAAATACAG 2461 TCCAAGATGC ATCTCATATC CCCACGTATG CACCCATCCC AGCTTAACCG CTTTGTAAAC 2521 ACTTACACCA AGGGCCTGCC CCTGGCAATC AGCTTGCTAC TGAAAGACAT TTTTAGGCAC 2581 CACGCCCAGC GCTCCTGCTA CGACTGGATC ATCTACAACA CCACCCCGCA GCATGAAGCT 2641 CTGCAGTGGT GCTACCTCCA CCCCAGAGAC GGGCTTATGC CCATGTATCT GAACATCCAG 2701 AGTCACCTTT ACCACGTCCT GGAAAAAATA CACAGGACCC TCAACGACCG AGACCGCTGG 2761 TCCCGGGCCT ACCGCGCGCG CAAAACCCCT AAATAAAGAC AGCAAGACAC TTGCTTGATC 2821 CAAATCCAAA CAGAGTCTGG TTTTTTATTT ATGTTTTAAA CCGCATTGGG AGGGGAGGAA 2881 GCCTTCAGGG CAGAAACCTG CTGGCGCAGA TCCAACAGCT GCTGAGAAAC GACATTAAGT 2941 TCCCGGGTCA AAGAATCCAA TTGTGCCAAA AGAGCCGTCA ACTTGTCATC GCGGGCGGAT 3001 GAACGGGAAG CTGCACTGCT TGCAAGCGGG CTCAGGAAAG CAAAGTCAGT CACAATCCCG 3061 CGGGCGGTGG CTGCAGCGGC TGAAGCGGCG GCGGAGGCTG CAGTCTCCAA CGGCGTTCCA 3121 GACACGGTCT CGTAGGTCAA GGTAGTAGAG TTTGCGGGCA GGACGGGGCG ACCATCAATG 3181 CTGGAGCCCA TCACATTCTG ACGCACCCCG GCCCATGGGG GCATGCGCGT TGTCAAATAT 3241 GAGCTCACAA TGCTTCCATC AAACGAGTTG GTGCTCATGG CGGCGGCGGC TGCTGCAAAA 3301 CAGATACAAA ACTACATAAG ACCCCCACCT TATATATTCT TTCCCACCCT TAACCACGCC 3361 CAGATCCTCT AGCAGTGATA AACGTCTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 3421 GCCCATATAT GGAGTTCCGA CGCGGAGGTT TATCGACGAT CTGCTAGTGA TTAATAGTAA 3481 TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG 3541 GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG 3601 TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA 3661 CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT 3721 GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC 3781 TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT 3841 TGGCAGTACA TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC 3901 CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT 3961 CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT 4021 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGTC GCCACCatgt 4081 cggacacgtg gagctctatc caggcccata agaaacaact ggactcgctt cgcgagagac 4141 tgcagcggcg gcggaagcag gactctgggc acttggactt aaggaatcca gaagcagcac 4201 tgtccccaac cttccgtagt gatagcccag tgcctactgc ccctacctct agtggcccta 4261 agcccagcac aacgtctata gtccctgaat tagctacaga ccctgagtta gagaagaagt 4321 tgctacacca cctctcagat ctcgccttaa ccttgcccac tgatgctgta tccatccgtc 4381 ttgccatctc tacgccagat gcacctgcca ctcaagatgg ggtagaaagc cttctacaga 4441 aatttgctgc ccaggagttg attgaggtaa agcgaggtct cctacaagat gacgcacatc 4501 ccactctcgt aacctatgct gaccactcca agctgtctgc catgatgggg gctgtggcag 4561 aaaagaaagg ccttggagag gcagcaggga ctatcacagg gcagaaacgg cgtgcagagc 4621 aggacttgac tacagtggct acctttacca gctctttagc atctggtctg ggctcttcaa 4681 catcagaacc agctaaggag ccagctaaga aatcaaggaa gcacgctgcc tcagatgttg 4741 acctggagat agaaagtctt ttgaaccaac agtcaactaa ggaacagcag agcaagaagg 4801 tcagtcagga gatcctagag ctattaaata ccacaacagc caaggaacag tccattgttg 4861 aaaagttccg ctctcgaggt cgggcccagg tgcaagaatt ttgtgactat gggaccaagg 4921 aagagtgcat gaaagccagt gacgctgatc ggccttgtcg caagctgcac ttcagacgga 4981 ttatcaacaa gcacactgat gagtctttag gtgattgctc tttcctgaac acctgcttcc 5041 acatggacac ctgcaagtat gtccactatg aaattgacgc ctgtgtggat tctgagagtc 5101 ctggcagcaa ggagcacatg ccgagccagg agcttgctct tacacagagc gttgggggcg 5161 actccagtgc agaccgactc tttccacccc agtggatctg ttgtgatatc cgctacctgg 5221 acgtcagtat cttgggcaaa tttgcagttg tgatggctga cccaccttgg gatattcaca 5281 tggagctacc gtatgggacg ttaacagatg acgagatgcg caggctcaat ataccagtac 5341 tacaggatga tggctttctt ttcctctggg tcacaggcag ggccatggaa ttgggcagag 5401 aatgtctgaa cctctggggt tacgagcggg tggacgagat catctgggtg aagaccaatc 5461 agctgcagcg catcatcagg acaggccgga caggccactg gctgaaccac gggaaggagc 5521 actgcttggt tggtgtcaaa ggaaatcccc aaggcttcaa ccagggtctg gattgcgatg 5581 tgattgtagc tgaggttcgt tccaccagtc acaaaccaga tgaaatatat ggcatgattg 5641 agagactgtc ccctggcacc cgaaagattg agttatttgg acgaccacac aatgtgcagc 5701 ccaactggat tactcttgga aaccaactgg atgggataca ccttctggac ccagatgtgg 5761 ttgcccggtt taagcacagg tatccagatg gtgtcatctc taaacctaag aacttatagT 5821 CCGGACTCAG ATCCACCGGA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG 5881 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA 5941 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 6001 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 6061 AACTCATCAA TGTATCTTAA CGCGATCAAG CTAGCTTGCT AGACTCGACT GACTATAATA 6121 ATAAAACGCC AACTTTGACC CGGAACGCGG AAAACACCTG AGAAAAACAC CTGGGCGAGT 6181 CTCCACGTAA ACGGTCAAAG TCCCCGCGGC CCTAGACAAA TATTACGCGC TATGAGTAAC 6241 ACAAAATTAT TCAGATTTCA CTTCCTCTTA TTCAGTTTTC CCGCGAAAAT GGCCAAATCT 6301 TACTCGGTTA CGCCCAAATT TACTACAACA TCCGCCTAAA ACCGCGCGAA AATTGTCACT 6361 TCCTGTGTAC ACCGGCGCAC ACCAAAAACG TCACTTTTGC CACATCCGTC GCTTACATGT 6421 GTTCCGCCAC ACTTGCAACA TCACACTTCC GCCACACTAC TACGTCACCC GCCCCGTTCC 6481 CACGCCCCGC GCCACGTCAC AAACTCCACC CCCTCATTAT CATATTGGCT TCAATCCAAA 6541 ATAAGGTATA TTATTGATGA TGTTAATTAA GAATTAATTC GATCCTGAAT GGCGAATGGA 6601 CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC 6661 TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC 6721 GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG 6781 TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC 6841 ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG 6901 ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA 6961 AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTTA 7021 ACAAAATTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG 7081 CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA 7141 TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGGTCCGC CACACCCAGC CGGCCACAGT 7201 CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT 7261 GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG 7321 CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA 7381 TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG 7441 GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG 7501 CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC 7561 CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG 7621 ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA 7681 AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG 7741 TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT 7801 GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCAGAGCTTG 7861 ATCCCCTGCG CCATCAGATC CTTGGCGGCA AGAAAGCCAT CCAGTTTACT TTGCAGGGCT 7921 TCCCAACCTT ACCAGAGGGC GCCCCAGCTG GCAATTCCGG TTCGCTTGCT GTCCATAAAA 7981 CCGCCCAGTC TAGCTATCGC CATGTAAGCC CACTGCAAGC TACCTGCTTT CTCTTTGCGC 8041 TTGCGTTTTC CCTTGTCCAG ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT 8101 TCTGCGGACT GGCTTTCTAC GTGAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA 8161 TGGCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG 8221 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC 8281 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT 8341 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT 8401 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG 8461 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG 8521 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA 8581 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA 8641 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA 8701 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA 8761 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT 8821 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT 8881 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT 8941 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT 9001 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA 9061 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG 9121 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC 9181 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA 9241 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA 9301 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG 9361 CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG 9421 AAG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.