pAN7-1 Plasmid
PVT5301 2ug
pAN7- 1 Plasmid information
Promoter: gpdA
Replicon: pUC ori
Terminator: TRPC
Plasmids: other host vectors; filamentous fungal expression plasmids
Plasmid size: 6756bp
Prokaryotic resistance: ampicillin Ampicillin
Screening markers: hygromycin Hygromycin
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expression host: filamentous fungi
Culture conditions: Reference Literature
Induction mode: no induction
5'sequencing primers: Based on full sequence design
3'sequencing primers: Based on full sequence design
Note: NCBI: Z32698.1
Use:Filamentous fungal plasmid
pAN7- 1 Plasmid Description
Reference
1、Effect of de-phosphorylation of linearized pAN7-1 and of addition of restriction enzyme on plasmid integration in Penicillium paxilli.
2、Transformation of sapstaining fungi with hygromycin B resistance plasmids pAN7-1 and pCB1004.
3、Padro de integrao de pAN7-1 em mutantes de Magnaporthe grisea com patogenicidade alterada em arroz.
4、Integração de pAN7-1 no Genoma de Magnaporthe grisea Mediada por Enzima de Restrição.
5、Primary structure of the trpC gene from Aspergillus nidulans.
6、Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.
7、Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase
pAN7- 1 Plasmid Sequence
LOCUS Z32698 6756 bp DNA linear SYN 14-NOV-2006
DEFINITION Expression vector (PAN7-1) DNA, 6756bp.
ACCESSION Z32698
VERSION Z32698.1 GI:475166
KEYWORDS hygromycin b phosphotransferase.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1
AUTHORS Mullaney,E.J., Hamer,J.E., Roberti,K.A., Yelton,M.M. and
Timberlake,W.E.
TITLE Primary structure of the trpC gene from Aspergillus nidulans
JOURNAL Mol. Gen. Genet. 199 (1), 37-45 (1985)
PUBMED 3158796
REMARK sites .
REFERENCE 2
AUTHORS Punt,P.J., Oliver,R.P., Dingemanse,M.A., Pouwels,P.H. and van den
Hondel,C.A.
TITLE Transformation of Aspergillus based on the hygromycin B resistance
marker from Escherichia coli
JOURNAL Gene 56 (1), 117-124 (1987)
PUBMED 2824287
REMARK (sites)
REFERENCE 3 (bases 999 to 2572)
AUTHORS Punt,P.J., Dingemanse,M.A., Kuyvenhoven,A., Soede,R.D.,
Pouwels,P.H. and van den Hondel,C.A.
TITLE Functional elements in the promoter region of the Aspergillus
nidulans gpdA gene encoding glyceraldehyde-3-phosphate
dehydrogenase
JOURNAL Gene 93 (1), 101-109 (1990)
PUBMED 2121607
REFERENCE 4 (bases 1 to 6756)
AUTHORS Punt,P.
TITLE Direct Submission
JOURNAL Submitted (22-APR-1994) Punt P., TNO Nutrition and Food Research
Institute, Lange Kleiweg 139, Rijkswik, The Netherlands
FEATURES Location/Qualifiers
source 1..6756
/organism="synthetic construct"
/mol_type="other DNA"
/db_xref="taxon:32630"
misc_feature 1..6756
/product="fungal transformation vector pAN7-1"
/standard_name="HPH"
/experiment="experimental evidence, no additional details
recorded"
/citation=[2]
/phenotype="gene product confers resistance to hygromycin
B"
regulatory 1..2129
/regulatory_class="promoter"
/experiment="experimental evidence, no additional details
recorded"
/citation=[2]
/citation=[3]
/function="promoter region of the A.nidulans gpdA gene"
gene 2130
/gene="gpdA"
misc_feature 2130
/gene="gpdA"
/experiment="experimental evidence, no additional details
recorded"
/citation=[3]
/function="major transcription start site"
intron 2178..2293
/experiment="experimental evidence, no additional details
recorded"
/citation=[3]
/number=1
gene 2302..3321
/gene="HPH"
CDS 2302..3321
/gene="HPH"
/experiment="experimental evidence, no additional details
recorded"
/note="gene-product confers resistance to hygromycin B"
/citation=[2]
/citation=[3]
/codon_start=1
/transl_table=11
/product="hygromycin B phosphotransferase"
/protein_id="CAA83647.1"
/db_xref="GI:475167"
/translation="MPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEA
MFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSL
VDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTR
PRAKE"
regulatory 3339..4108
/regulatory_class="terminator"
/standard_name="terminator region from A.nidulans trpC
gene (containing CDS)"
/experiment="experimental evidence, no additional details
recorded"
/note="this region contains the 3'end of the trpC
transcript including 50 codons. two major transcription
ends have been identified"
/citation=[1]
/citation=[2]
polyA_site 3590..3600
misc_difference 3600..3610
/note="introduction of one base at this position generates
an BLGI site with was shown to be present by restriction
digestion"
/citation=[1]
/replace="gccttncaggc"
misc_difference 3809..3817
/note="introduction of one base at this position generates
an ALWNI site which was shown to be present by restriction
digestion"
/citation=[1]
/replace="cagcncctg"
polyA_site 3863..3873
misc_difference 3897..3907
/note="introduction (actual sequence could be deletion as
well) of a base at this position results in the
elimination of an XMNI site which was shown to be absent
by restriction digestion"
/citation=[1]
/replace="gaaatcanttc"
variation 3934..3941
/note="A NCOI site present at this position was removed by
digestion, treatment with klenow polymerase and
religation"
/citation=[2]
/replace="catgcatg"
misc_feature 4105..6756
/product="PUC18"
/note="vector sequences derived from PUC18 from SALI TO
ECORI"
/citation=[2]
ORIGIN
1 gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
61 cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
121 cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
181 agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
241 cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
301 ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
361 tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
421 ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
481 ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
541 cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
601 ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
661 gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
721 ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
781 ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
841 acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
901 gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
961 catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
1021 aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
1081 gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
1141 aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
1201 ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
1261 aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
1321 gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
1381 cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
1441 ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
1501 gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
1561 ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
1621 atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
1681 ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
1741 ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
1801 ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
1861 ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
1921 cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
1981 gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
2041 tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
2101 ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
2161 acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
2221 attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
2281 cccgcttgag cagacatcac catgcctgaa ctcaccgcga cgtctgtcga gaagtttctg
2341 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
2401 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
2461 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
2521 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
2581 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
2641 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
2701 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
2761 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
2821 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
2881 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
2941 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
3001 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
3061 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
3121 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
3181 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
3241 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
3301 cgtccgaggg caaaggaata gagtagatgc cgaccgcggg atccacttaa cgttactgaa
3361 atcatcaaac agcttgacga atctggatat aagatcgttg gtgtcgatgt cagctccgga
3421 gttgagacaa atggtgttca ggatctcgat aagatacgtt catttgtcca agcagcaaag
3481 agtgccttct agtgatttaa tagctccatg tcaacaagaa taaaacgcgt tttcgggttt
3541 acctcttcca gatacagctc atctgcaatg cattaatgca ttgactgcaa cctagtaacg
3601 ccttncaggc tccggcgaag agaagaatag cttagcagag ctattttcat tttcgggaga
3661 cgagatcaag cagatcaacg gtcgtcaaga gacctacgag actgaggaat ccgctcttgg
3721 ctccacgcga ctatatattt gtctctaatt gtactttgac atgctcctct tctttactct
3781 gatagcttga ctatgaaaat tccgtcacca gcncctgggt tcgcaaagat aattgcatgt
3841 ttcttccttg aactctcaag cctacaggac acacattcat cgtaggtata aacctcgaaa
3901 tcanttccta ctaagatggt atacaatagt aaccatgcat ggttgcctag tgaatgctcc
3961 gtaacaccca atacgccggc cgaaactttt ttacaactct cctatgagtc gtttacccag
4021 aatgcacagg tacacttgtt tagaggtaat ccttctttct agaagtcctc gtgtactgtg
4081 taagcgccca ctccacatct ccactcgacc tgcaggcatg caagcttggc actggccgtc
4141 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
4201 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
4261 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
4321 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
4381 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
4441 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
4501 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
4561 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
4621 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
4681 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
4741 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
4801 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
4861 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
4921 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
4981 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
5041 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
5101 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
5161 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
5221 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
5281 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
5341 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
5401 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
5461 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
5521 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
5581 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
5641 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
5701 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
5761 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
5821 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
5881 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
5941 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
6001 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
6061 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
6121 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
6181 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
6241 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
6301 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
6361 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
6421 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
6481 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
6541 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
6601 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
6661 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
6721 caatttcaca caggaaacag ctatgaccat gattac
//
Caution:
- This product is FOR RESEARCH USE ONLY!
- The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
- Shipping temperature is 2-8℃.
Search name
pAN7-1,Plasmid pAN7-1,pAN7-1 vector
English