pB4GALT2-Fluc plasmid

PVT110503

pB4GALT2-Fluc plasmid Information

Plasmid name: pB4GALT2(human)-Fluc
Alias:
Gene lenght: 1100
Host: Mammalian cells
Use(s): Signal report
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: B4GALT2
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: RVP3
Reverse primer:
Copy number: High
Induction:

pB4GALT2-Fluc plasmid Description

pB4GALT2-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pB4GALT2-Fluc plasmid Sequence

1 GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT GTGTGAATCG 61 ATAGTACTAA CATACGCTCT CCATCAAAAC AAAACGAAAC AAAACAAACT AGCAAAATAG 121 GCTGTCCCCA GTGCAAGTGC AGGTGCCAGA ACATTTCTCT ATCGATAGGT ACCtgcagct 181 gcgcacctgt gtcccccacc tccaccctca acccatcttc ctagtgtttg tgaaataaac 241 ttggtatttg tctgggtcag tgcagcttct gttgccctct tgcgtcaccg ttcacagagg 301 aagggtaaag aggcctgtac gcctcagcct catgccaatc ccttgtcagc tttctgactg 361 cacacttctg atctgcacag agcttgctcc taggtgggga ggcacgggtg ctgggtggac 421 tctaaccccc aaacagactt gtttggggat gagggagtgt gcagataggg gcccctgggt 481 gtgtttgtgc ctgtagtcct tctaggcccg tttgctcagg gctctgactg accaccatgc 541 ccgaattgtc tgacagcatc tacttacctg gttgtacagt cgggcaccat catctccgtc 601 cttgtgaggc ctggagccta ggcagtggtc tctgcctcca gtgatggtcc tgtccttcta 661 gcagcagccc ctcccagaaa cctctactct gatccttgtt cccagggccc agggcagctc 721 ctcccacccc atccaatctc gttttctcct tccctgaccc tgtttcctgt taccccagaa 781 gccccagcca cctccagccc acgcctttaa gggtcctgcc ccctctctgc tggccgacct 841 tgaatcttgg tccaattaaa gagttgacaa atccctagcc ccgcagcctg agtccgccca 901 acctccaggt ccccttcctg gggagttcgc gctccaatgc aatccccact ccccgctaag 961 ggactccacg gtctgcaccc gctcgccggt ccgacctcta agcccaccct ttggacgagc 1021 ctgacccatt tcctcccgcg ctcccaggtc cgtgcctcct gggtgcccgg gttcggccgc 1081 cggcagggcg ggctcgcagc ggcgcgcggc gggtgcgggc gtgctggagg cgggggccgc 1141 gggggcgcgc gcgcgggcgc gcgggccggg cctGCCCCTC CGAGGCGCCG TAGCGCGGGA 1201 GGGAGGCGGC GGCGCTGTGG TCCGTGGGTC CGCCGGTCCG TGGGTCTGCC CGGCCGCCCG 1261 GCCCCGCCCT GCCCTCGAGA TCTGCGATCT AAGTAAGCTT GGCATTCCGG TACTGTTGGT 1321 AAAGCCACCA TGGAAGACGC CAAAAACATA AAGAAAGGCC CGGCGCCATT CTATCCGCTG 1381 GAAGATGGAA CCGCTGGAGA GCAACTGCAT AAGGCTATGA AGAGATACGC CCTGGTTCCT 1441 GGAACAATTG CTTTTACAGA TGCACATATC GAGGTGGACA TCACTTACGC TGAGTACTTC 1501 GAAATGTCCG TTCGGTTGGC AGAAGCTATG AAACGATATG GGCTGAATAC AAATCACAGA 1561 ATCGTCGTAT GCAGTGAAAA CTCTCTTCAA TTCTTTATGC CGGTGTTGGG CGCGTTATTT 1621 ATCGGAGTTG CAGTTGCGCC CGCGAACGAC ATTTATAATG AACGTGAATT GCTCAACAGT 1681 ATGGGCATTT CGCAGCCTAC CGTGGTGTTC GTTTCCAAAA AGGGGTTGCA AAAAATTTTG 1741 AACGTGCAAA AAAAGCTCCC AATCATCCAA AAAATTATTA TCATGGATTC TAAAACGGAT 1801 TACCAGGGAT TTCAGTCGAT GTACACGTTC GTCACATCTC ATCTACCTCC CGGTTTTAAT 1861 GAATACGATT TTGTGCCAGA GTCCTTCGAT AGGGACAAGA CAATTGCACT GATCATGAAC 1921 TCCTCTGGAT CTACTGGTCT GCCTAAAGGT GTCGCTCTGC CTCATAGAAC TGCCTGCGTG 1981 AGATTCTCGC ATGCCAGAGA TCCTATTTTT GGCAATCAAA TCATTCCGGA TACTGCGATT 2041 TTAAGTGTTG TTCCATTCCA TCACGGTTTT GGAATGTTTA CTACACTCGG ATATTTGATA 2101 TGTGGATTTC GAGTCGTCTT AATGTATAGA TTTGAAGAAG AGCTGTTTCT GAGGAGCCTT 2161 CAGGATTACA AGATTCAAAG TGCGCTGCTG GTGCCAACCC TATTCTCCTT CTTCGCCAAA 2221 AGCACTCTGA TTGACAAATA CGATTTATCT AATTTACACG AAATTGCTTC TGGTGGCGCT 2281 CCCCTCTCTA AGGAAGTCGG GGAAGCGGTT GCCAAGAGGT TCCATCTGCC AGGTATCAGG 2341 CAAGGATATG GGCTCACTGA GACTACATCA GCTATTCTGA TTACACCCGA GGGGGATGAT 2401 AAACCGGGCG CGGTCGGTAA AGTTGTTCCA TTTTTTGAAG CGAAGGTTGT GGATCTGGAT 2461 ACCGGGAAAA CGCTGGGCGT TAATCAAAGA GGCGAACTGT GTGTGAGAGG TCCTATGATT 2521 ATGTCCGGTT ATGTAAACAA TCCGGAAGCG ACCAACGCCT TGATTGACAA GGATGGATGG 2581 CTACATTCTG GAGACATAGC TTACTGGGAC GAAGACGAAC ACTTCTTCAT CGTTGACCGC 2641 CTGAAGTCTC TGATTAAGTA CAAAGGCTAT CAGGTGGCTC CCGCTGAATT GGAATCCATC 2701 TTGCTCCAAC ACCCCAACAT CTTCGACGCA GGTGTCGCAG GTCTTCCCGA CGATGACGCC 2761 GGTGAACTTC CCGCCGCCGT TGTTGTTTTG GAGCACGGAA AGACGATGAC GGAAAAAGAG 2821 ATCGTGGATT ACGTCGCCAG TCAAGTAACA ACCGCGAAAA AGTTGCGCGG AGGAGTTGTG 2881 TTTGTGGACG AAGTACCGAA AGGTCTTACC GGAAAACTCG ACGCAAGAAA AATCAGAGAG 2941 ATCCTCATAA AGGCCAAGAA GGGCGGAAAG ATCGCCGTGT AATTCTAGAG TCGGGGCGGC 3001 CGGCCGCTTC GAGCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA 3061 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC 3121 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT 3181 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTAAAATC 3241 GATAAGGATC CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG 3301 GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT 3361 AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 3421 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 3481 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 3541 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 3601 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 3661 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 3721 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 3781 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 3841 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 3901 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 3961 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 4021 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 4081 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 4141 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 4201 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 4261 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 4321 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 4381 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 4441 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 4501 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 4561 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 4621 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 4681 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 4741 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 4801 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 4861 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 4921 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 4981 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 5041 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 5101 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 5161 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 5221 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 5281 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 5341 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 5401 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 5461 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 5521 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 5581 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 5641 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 5701 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC 5761 TTACAATTTG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG 5821 CCTCTTCGCT ATTACGCCAG CCCAAGCTAC CATGATAAGT AAGTAATATT AAGGTACGGG 5881 AGGTACTTGG AGC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.