pBiFC-VC155
Catalog No. PVT10840
Packing 2ug
pBiFC-VC155 Information
Promoter: CMV
Replicon: PUC
Terminator: SV40 poly (a) signal
Plasmid classification: mammalian cells, bimolecular fluorescent complementary vector
Plasmid size: 4088 BP
Prokaryotic resistance: amp
Clone strain: dh5a
Culture conditions: 37 degrees
Expression host: mammalian cells
Induction mode: transient expression without induction
5 'sequencing primer: cmv-f: cgcaaaatgggcgtgtgtgggtgggtg
3 'sequencing primer: sv40-polya-r: gaaatttgatgctattgc
Plasmid host: mammalian cells
Plasmid usage: signal report
Fragment type:
Fragment species:
Prokaryotic resistance: amp
pBiFC-VC155 Description
PBiFC-VC155 by pCMV-HA vector in KpnI and inserted at the NotI locus of VC155 gene, the plasmid is high copy plasmid, VC155 gene derived from Vitoria victoria. Visualization of Aequorea Aequorea ternary complexes in living cells by using a BiFC-based FRET analysis.
Nature Protocols, 3:1693-1702 (2008).
pBiFC-VC155 Sequence
LOCUS Exported 4088 bp ds-DNA circular SYN 29-JUN-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBiFC-VC155
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4088)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sunday, September 4, 2016 from SnapGene Viewer 3.1.4
http://www.maiolingbio.com
FEATURES Location/Qualifiers
source 1..4088
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 4..30
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
misc_feature 82..384
/note="VC155"
polyA_signal 399..533
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(644..660)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 668..684
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(692..722)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 737..758
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1046..1634)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1805..2665)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2666..2770)
/gene="bla"
/note="AmpR promoter"
primer_bind 3244..3260
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
enhancer 3287..3590
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 3591..3794
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 3932..4028
/note="SV40 intron"
/note="modified SV40 intron with splice donor and acceptor
sites"
ORIGIN
1 atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg aattcggtcg
61 accgagatct ctcgaggtac ccgtccggcg tgcaaaatcc cgaacgacct gaaacagaaa
121 gtcatgaacc acgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
181 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
241 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaaact gagcaaagac
301 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
361 ctcggcatgg acgagctgta caagtaagcg gccgcgggga tccagacatg ataagataca
421 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
481 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
541 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttcggatc
601 ctctagagtc gatctgcagg catgctagct tggcgtaatc atggtcatag ctgtttcctg
661 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
721 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
781 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
841 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
901 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
961 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
1021 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
1081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
1141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
1201 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
1261 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
1321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
1381 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
1441 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
1501 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
1561 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
1621 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
1681 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
1741 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
1801 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
1861 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
1921 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
1981 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
2041 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
2101 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
2161 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
2221 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
2281 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
2341 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
2401 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
2461 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
2521 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
2581 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
2641 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
2701 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
2761 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
2821 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
2881 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
2941 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
3001 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
3061 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
3121 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
3181 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
3241 gttgtaaaac gacggccagt gagttcgagc ttgcatgcct gcaggtcgtt acataactta
3301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
3361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
3421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
3481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
3541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
3601 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
3661 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
3721 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
3781 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
3841 ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagag gatccggtac
3901 tagaggaact gaaaaaccag aaagttaact ggtaagttta gtctttttgt cttttatttc
3961 aggtcccgga tccggtggtg gtgcaaatca aagaactgct cctcagtgga tgttgccttt
4021 acttctaggc ctgtacggaa gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg
4081 ggcccacc
//
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