pBiT2.1-ADAM12-TK-SmBiT plasmid

PVT106382

pBiT2.1-ADAM12-TK-SmBiT plasmid Information

Plasmid name: pBiT2.1-ADAM12(human)-TK-SmBiT
Alias: NM_001288973.2;ADAM12-OT1; CAR10; MCMP; MCMPMltna; MLTN;MLTNA
Gene lenght: 2718
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: TK
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: HSV TK-F1
Reverse primer:
Copy number: High
Induction:

pBiT2.1-ADAM12-TK-SmBiT plasmid Description

pBiT2.1-ADAM12-TK-SmBiT is a plasmid suitable for protein expression in mammalian cells.

pBiT2.1-ADAM12-TK-SmBiT plasmid Sequence

1 GGCCTAACTG GCCGGTACCT GAGTCTAAAT GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA 61 GCGGTGGTTA GGGTTTGTCT GACGCGGGGG GAGGGGGAAG GAACGAAACA CTCTCATTCG 121 GAGGCGGCTC GGGGTTTGGT CTTGGTGGCC ACGGGCACGC AGAAGAGCGC CGCGATCCTC 181 TTAAGCACCC CCCCGCCCTC CGTGGAGGCG GGGGTTTGGT CGGCGGGTGG TAACTGGCGG 241 GCCGCTGACT CGGGCGGGTC GCGCGCCCCA GAGTGTGACC TTTTCGGTCT GCTCGCAGAC 301 CCCCGGGCGG CGCCGCCGCG GCGGCGACGG GCTCGCTGGG TCCTAGGCTC CATGGGGACC 361 GTATACGTGG ACAGGCTCTG GAGCATCCGC ACGACTGCGG TGATATTACC GGAGACCTTC 421 TGCGGGACGA GCCGGGTCAC GCGGCTGACG CGGAGCGTCC GTTGGGCGAC AAACACCAGG 481 ACGGGGCACA GGTACACTAT CTTGTCACCC GGAGGCGCGA GGGACTGCAG GAGCTTCAGG 541 GAGTGGCGCA GCTGCTTCAT CCCCGTGGCC CGTTGCTCGC GTTTGCTGGC GGTGTCCCCG 601 GAAGAAATAT ATTTGCATGT CTTTAGTTCT ATGATGACAC AAACCCCGCC CAGCGTCTTG 661 TCATTGGCGA AGTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG 721 CATATTAAGG TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCGA CCCGCTTAAA 781 AGCTTGGCAA TCCGGTACTG TGGTAAAGCC ACCAGATCTG CTAGCGATCG CCTAAGTGGG 841 AGCTCAGGGG AATTCcgcca ccatggcagc gcgcccgctg cccgtgtccc ccgcccgcgc 901 cctcctgctc gccctggccg gtgctctgct cgcgccctgc gaggcccgag gggtgagctt 961 atggaaccaa ggaagagctg atgaagttgt cagtgcctct gttgggagtg gggacctctg 1021 gatcccagtg aagagcttcg actccaagaa tcatccagaa gtgctgaata ttcgactaca 1081 acgggaaagc aaagaactga tcataaatct ggaaagaaat gaaggtctca ttgccagcag 1141 tttcacggaa acccactatc tgcaagacgg tactgatgtc tccctcgctc gaaattacac 1201 gggtcactgt tactaccatg gacatgtacg gggatattct gattcagcag tcagtctcag 1261 cacgtgttct ggtctcaggg gacttattgt gtttgaaaat gaaagctatg tcttagaacc 1321 aatgaaaagt gcaaccaaca gatacaaact cttcccagcg aagaagctga aaagcgtccg 1381 gggatcatgt ggatcacatc acaacacacc aaacctcgct gcaaagaatg tgtttccacc 1441 accctctcag acatgggcaa gaaggcataa aagagagacc ctcaaggcaa ctaagtatgt 1501 ggagctggtg atcgtggcag acaaccgaga gtttcagagg caaggaaaag atctggaaaa 1561 agttaagcag cgattaatag agattgctaa tcacgttgac aagttttaca gaccactgaa 1621 cattcggatc gtgttggtag gcgtggaagt gtggaatgac atggacaaat gctctgtaag 1681 tcaggaccca ttcaccagcc tccatgaatt tctggactgg aggaagatga agcttctacc 1741 tcgcaaatcc catgacaatg cgcagcttgt cagtggggtt tatttccaag ggaccaccat 1801 cggcatggcc ccaatcatga gcatgtgcac ggcagaccag tctgggggaa ttgtcatgga 1861 ccattcagac aatccccttg gtgcagccgt gaccctggca catgagctgg gccacaattt 1921 cgggatgaat catgacacac tggacagggg ctgtagctgt caaatggcgg ttgagaaagg 1981 aggctgcatc atgaacgctt ccaccgggta cccatttccc atggtgttca gcagttgcag 2041 caggaaggac ttggagacca gcctggagaa aggaatgggg gtgtgcctgt ttaacctgcc 2101 ggaagtcagg gagtctttcg ggggccagaa gtgtgggaac agatttgtgg aagaaggaga 2161 ggagtgtgac tgtggggagc cagaggaatg tatgaatcgc tgctgcaatg ccaccacctg 2221 taccctgaag ccggacgctg tgtgcgcaca tgggctgtgc tgtgaagact gccagctgaa 2281 gcctgcagga acagcgtgca gggactccag caactcctgt gacctcccag agttctgcac 2341 aggggccagc cctcactgcc cagccaacgt gtacctgcac gatgggcact catgtcagga 2401 tgtggacggc tactgctaca atggcatctg ccagactcac gagcagcagt gtgtcacgct 2461 ctggggacca ggtgctaaac ctgcccctgg gatctgcttt gagagagtca attctgcagg 2521 tgatccttat ggcaactgtg gcaaagtctc gaagagttcc tttgccaaat gcgagatgag 2581 agatgctaaa tgtggaaaaa tccagtgtca aggaggtgcc agccggccag tcattggtac 2641 caatgccgtt tccatagaaa caaacatccc cctgcagcaa ggaggccgga ttctgtgccg 2701 ggggacccac gtgtacttgg gcgatgacat gccggaccca gggcttgtgc ttgcaggcac 2761 aaagtgtgca gatggaaaaa tctgcctgaa tcgtcaatgt caaaatatta gtgtctttgg 2821 ggttcacgag tgtgcaatgc agtgccacgg cagaggggtg tgcaacaaca ggaagaactg 2881 ccactgcgag gcccactggg cacctccctt ctgtgacaag tttggctttg gaggaagcac 2941 agacagcggc cccatccggc aagcagataa ccaaggttta accataggaa ttctggtgac 3001 catcctgtgt cttcttgctg ccggatttgt ggtttatctc aaaaggaaga ccttgatacg 3061 actgctgttt acaaataaga agaccaccat tgaaaaacta aggtgtgtgc gcccttcccg 3121 gccaccccgt ggcttccaac cctgtcaggc tcacctcggc caccttggaa aaggcctgat 3181 gaggaagccg ccagattcct acccaccgaa ggacaatccc aggagattgc tgcagtgtca 3241 gaatgttgac atcagcagac ccctcaacgg cctgaatgtc cctcagcccc agtcaactca 3301 gcgagtgctt cctcccctcc accgggctcc acgtgcacct agcgtccctg ccagacccct 3361 gccagccaag cctgcactta ggcaggccca ggggacctgt aagccaaacc cccctcagaa 3421 gcctctgcct gcagatcctc tggccagaac aactcggctc actcatgcct tggccaggac 3481 cccaggacaa tgggagactg ggctccgcct ggcacccctc agacctgctc cacaatatcc 3541 acaccaagtg cccagatcca cccacaccgc ctatattaag CTCGAGGGTG GTGGCGGGAG 3601 CGGAGGTGGA GGGTCGTCAG GTGTGACCGG CTACCGGCTG TTCGAGGAGA TTCTGTAATC 3661 TAGAGTCGGG GCGGCCGGCC GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA 3721 CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT 3781 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT 3841 TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC 3901 AAATGTGGTA AAATCGATAA GGATCCGTCG ACCGATGCCC TTGAGAGCCT TCAACCCAGT 3961 CAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT 4021 TATCATGCAA CTCGTAGGAC AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT 4081 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 4141 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 4201 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 4261 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 4321 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 4381 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 4441 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 4501 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 4561 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 4621 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 4681 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 4741 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 4801 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 4861 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 4921 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 4981 AAACTTGGTC TGACAGCGGC CGCAAATGCT AAACCACTGC AGTGGTTACC AGTGCTTGAT 5041 CAGTGAGGCA CCGATCTCAG CGATCTGCCT ATTTCGTTCG TCCATAGTGG CCTGACTCCC 5101 CGTCGTGTAG ATCACTACGA TTCGTGAGGG CTTACCATCA GGCCCCAGCG CAGCAATGAT 5161 GCCGCGAGAG CCGCGTTCAC CGGCCCCCGA TTTGTCAGCA ATGAACCAGC CAGCAGGGAG 5221 GGCCGAGCGA AGAAGTGGTC CTGCTACTTT GTCCGCCTCC ATCCAGTCTA TGAGCTGCTG 5281 TCGTGATGCT AGAGTAAGAA GTTCGCCAGT GAGTAGTTTC CGAAGAGTTG TGGCCATTGC 5341 TACTGGCATC GTGGTATCAC GCTCGTCGTT CGGTATGGCT TCGTTCAACT CTGGTTCCCA 5401 GCGGTCAAGC CGGGTCACAT GATCACCCAT ATTATGAAGA AATGCAGTCA GCTCCTTAGG 5461 GCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCGGTGTTG TCGCTCATGG TAATGGCAGC 5521 ACTACACAAT TCTCTTACCG TCATGCCATC CGTAAGATGC TTTTCCGTGA CCGGCGAGTA 5581 CTCAACCAAG TCGTTTTGTG AGTAGTGTAT ACGGCGACCA AGCTGCTCTT GCCCGGCGTC 5641 TATACGGGAC AACACCGCGC CACATAGCAG TACTTTGAAA GTGCTCATCA TCGGGAATCG 5701 TTCTTCGGGG CGGAAAGACT CAAGGATCTT GCCGCTATTG AGATCCAGTT CGATATAGCC 5761 CACTCTTGCA CCCAGTTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CGGGGTGTGC 5821 AAAAACAGGC AAGCAAAATG CCGCAAAGAA GGGAATGAGT GCGACACGAA AATGTTGGAT 5881 GCTCATACTC GTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTACT AGTACGTCTC 5941 TCAAGGATAA GTAAGTAATA TTAAGGTACG GGAGGTATTG GACAGGCCGC AATAAAATAT 6001 CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGATAGTA CTAACATACG 6061 CTCTCCATCA AAACAAAACG AAACAAAACA AACTAGCAAA ATAGGCTGTC CCCAGTGCAA 6121 GTGCAGGTGC CAGAACATTT CTCT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???