pBiT2.1-ZEB1-TK-SmBiT plasmid

PVT106429

pBiT2.1-ZEB1-TK-SmBiT plasmid Information

Plasmid name: pBiT2.1-ZEB1(human)-TK-SmBiT
Alias: NM_001128128.3;AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;TCF8; ZFHEP; ZFHX1A
Gene lenght: 3324
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: TK
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: HSV TK-F1
Reverse primer:
Copy number: High
Induction:

pBiT2.1-ZEB1-TK-SmBiT plasmid Description

pBiT2.1-ZEB1-TK-SmBiT is a plasmid suitable for protein expression in mammalian cells.

pBiT2.1-ZEB1-TK-SmBiT plasmid Sequence

1 GGCCTAACTG GCCGGTACCT GAGTCTAAAT GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA 61 GCGGTGGTTA GGGTTTGTCT GACGCGGGGG GAGGGGGAAG GAACGAAACA CTCTCATTCG 121 GAGGCGGCTC GGGGTTTGGT CTTGGTGGCC ACGGGCACGC AGAAGAGCGC CGCGATCCTC 181 TTAAGCACCC CCCCGCCCTC CGTGGAGGCG GGGGTTTGGT CGGCGGGTGG TAACTGGCGG 241 GCCGCTGACT CGGGCGGGTC GCGCGCCCCA GAGTGTGACC TTTTCGGTCT GCTCGCAGAC 301 CCCCGGGCGG CGCCGCCGCG GCGGCGACGG GCTCGCTGGG TCCTAGGCTC CATGGGGACC 361 GTATACGTGG ACAGGCTCTG GAGCATCCGC ACGACTGCGG TGATATTACC GGAGACCTTC 421 TGCGGGACGA GCCGGGTCAC GCGGCTGACG CGGAGCGTCC GTTGGGCGAC AAACACCAGG 481 ACGGGGCACA GGTACACTAT CTTGTCACCC GGAGGCGCGA GGGACTGCAG GAGCTTCAGG 541 GAGTGGCGCA GCTGCTTCAT CCCCGTGGCC CGTTGCTCGC GTTTGCTGGC GGTGTCCCCG 601 GAAGAAATAT ATTTGCATGT CTTTAGTTCT ATGATGACAC AAACCCCGCC CAGCGTCTTG 661 TCATTGGCGA AGTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG 721 CATATTAAGG TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCGA CCCGCTTAAA 781 AGCTTGGCAA TCCGGTACTG TGGTAAAGCC ACCAGATCTG CTAGCGATCG CCTAAGTGGG 841 AGCTCAGGGG AATTCcgcca ccatgaaagt tacaaattat aatactgtgg tagaaacaaa 901 ttcagattca gatgatgaag acaaactgca tattgtggaa gaagaaagtg ttacagatgc 961 agctgactgt gaaggtgtac cagaggatga cctgccaaca gaccagacag tgttaccagg 1021 gaggagcagt gaaagagaag ggaatgctaa gaactgctgg gaggatgaca caggaaagga 1081 agggcaagaa atcctggggc ctgaagctca ggcagatgaa gcaggatgta cagtaaaaga 1141 tgatgaatgc gagtcagatg cagaaaatga gcaaaaccat gatcctaatg ttgaagagtt 1201 tctacaacaa caagacactg ctgtcatttt tcctgaggca cctgaagagg accagaggca 1261 gggcacacca gaagccagtg gtcatgatga aaatggaaca ccagatgcat tttcacaatt 1321 actcacctgt ccatattgtg atagaggcta taaacgcttt acctctctga aagaacacat 1381 taaatatcgt catgaaaaga atgaagataa ctttagttgc tccctgtgca gttacacctt 1441 tgcatacaga acccaacttg aacgtcacat gacatcacat aaatcaggaa gagatcaaag 1501 acatgtgacg cagtctgggt gtaatcgtaa attcaaatgc actgagtgtg gaaaagcttt 1561 caaatacaaa catcacctaa aagagcactt aagaattcac agtggagaga agccatatga 1621 atgcccaaac tgcaagaaac gcttttccca ttctggctcc tatagctcac acataagcag 1681 taagaaatgt atcagcttga tacctgtgaa tgggcgacca agaacaggac tcaagacatc 1741 tcagtgttct tcaccgtctc tttcagcatc accaggcagt cccacacgac cacagatacg 1801 gcaaaagata gagaataaac cccttcaaga acaactttct gttaaccaaa ttaaaactga 1861 acctgtggat tatgaattca aacccatagt ggttgcttca ggaatcaact gttcaacccc 1921 tttacaaaat ggggttttca ctggtggtgg cccattacag gcaaccagtt ctcctcaggg 1981 catggtgcaa gctgttgttc tgccaacagt tggtttggtg tctcccataa gtatcaattt 2041 aagtgatatt cagaatgtac ttaaagtggc ggtagatggt aatgtaataa ggcaagtgtt 2101 ggagaataat caagccaatc ttgcatccaa agaacaagaa acaatcaatg cttcacccat 2161 acaacaaggt ggccattctg ttatttcagc catcagtctt cctttggttg atcaagatgg 2221 aacaaccaaa attatcatca actacagtct tgagcagcct agccaacttc aagttgttcc 2281 tcaaaattta aaaaaagaaa atccagtcgc tacaaacagt tgtaaaagtg aaaagttacc 2341 agaagatctt actgttaagt ctgagaagga caaaagcttt gaaggggggg tgaatgatag 2401 cacttgtctt ctgtgtgatg attgtccagg agatattaat gcacttccag aattaaagca 2461 ctatgaccta aagcagccta ctcagcctcc tccactccct gcagcagaag ctgagaagcc 2521 tgagtcctct gtttcatcag ctactggaga tggcaatttg tctcctagtc agccaccttt 2581 aaagaacctc ttgtctctcc taaaagcata ttatgctttg aatgcacaac caagtgcaga 2641 agagctctca aaaattgctg attcagtaaa cctaccactg gatgtagtaa aaaagtggtt 2701 tgaaaagatg caagctggac agatttcagt gcagtcttct gaaccatctt ctcctgaacc 2761 aggcaaagta aatatccctg ccaagaacaa tgatcagcct caatctgcaa atgcaaatga 2821 accccaggac agcacagtaa atctacaaag tcctttgaag atgactaact ccccagtttt 2881 accagtggga tcaaccacca atggttccag aagtagtaca ccatccccat cacctctaaa 2941 cctttcctca tccagaaata cacagggtta cttgtacaca gctgagggtg cacaagaaga 3001 gccacaagta gaacctcttg atctttcact accaaagcaa cagggagaat tattagaaag 3061 gtcaactatc actagtgttt accagaacag tgtttattct gtccaggaag aacccttgaa 3121 cttgtcttgc gcaaaaaagg agccacaaaa ggacagttgt gttacagact cagaaccagt 3181 tgtaaatgta atcccaccaa gtgccaaccc cataaatatc gctataccta cagtcactgc 3241 ccagttaccc acaatcgtgg ccattgctga ccagaacagt gttccatgct taagagcgct 3301 agctgccaat aagcaaacga ttctgattcc ccaggtggca tacacctact caactacggt 3361 cagccctgca gtccaagaac cacccttgaa agtgatccag ccaaatggaa atcaggatga 3421 aagacaagat actagctcag aaggagtatc aaatgtagag gatcagaatg actctgattc 3481 tacaccgccc aaaaagaaaa tgcggaagac agaaaatgga atgtatgctt gtgatttgtg 3541 tgacaagata ttccaaaaga gtagttcatt attgagacat aaatatgaac acacaggtaa 3601 aagacctcat gagtgtggaa tctgtaaaaa ggcatttaaa cacaaacatc atttgattga 3661 acacatgcga ttacattctg gagaaaagcc ctatcaatgt gacaaatgtg gaaagcgctt 3721 ctcacactct gggtcttatt ctcaacacat gaatcatcgc tactcctact gtaagagaga 3781 agcggaagaa cgtgacagca cagagcagga agaggcaggg cctgaaatcc tctcgaatga 3841 gcacgtgggt gccagggcgt ctccctcaca gggcgactcg gacgagagag agagtttgac 3901 aagggaagag gatgaagaca gtgaaaaaga ggaagaggag gaggataaag agatggaaga 3961 attgcaggaa gaaaaagaat gtgaaaaacc acaaggggat gaggaagagg aggaggagga 4021 ggaagaagtg gaagaagaag aggtagaaga ggcagagaat gagggagaag aagcaaaaac 4081 tgaaggtctg atgaaggatg acagggctga aagtcaagca agcagcttag gacaaaaagt 4141 aggcgagagt agtgagcaag tgtctgaaga aaagacaaat gaagccCTCG AGGGTGGTGG 4201 CGGGAGCGGA GGTGGAGGGT CGTCAGGTGT GACCGGCTAC CGGCTGTTCG AGGAGATTCT 4261 GTAATCTAGA GTCGGGGCGG CCGGCCGCTT CGAGCAGACA TGATAAGATA CATTGATGAG 4321 TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT 4381 GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC 4441 ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC 4501 CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCGTCGACCG ATGCCCTTGA GAGCCTTCAA 4561 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT 4621 CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA 4681 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 4741 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 4801 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 4861 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 4921 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 4981 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 5041 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 5101 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 5161 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 5221 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 5281 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 5341 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 5401 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 5461 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 5521 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 5581 ATGAGTAAAC TTGGTCTGAC AGCGGCCGCA AATGCTAAAC CACTGCAGTG GTTACCAGTG 5641 CTTGATCAGT GAGGCACCGA TCTCAGCGAT CTGCCTATTT CGTTCGTCCA TAGTGGCCTG 5701 ACTCCCCGTC GTGTAGATCA CTACGATTCG TGAGGGCTTA CCATCAGGCC CCAGCGCAGC 5761 AATGATGCCG CGAGAGCCGC GTTCACCGGC CCCCGATTTG TCAGCAATGA ACCAGCCAGC 5821 AGGGAGGGCC GAGCGAAGAA GTGGTCCTGC TACTTTGTCC GCCTCCATCC AGTCTATGAG 5881 CTGCTGTCGT GATGCTAGAG TAAGAAGTTC GCCAGTGAGT AGTTTCCGAA GAGTTGTGGC 5941 CATTGCTACT GGCATCGTGG TATCACGCTC GTCGTTCGGT ATGGCTTCGT TCAACTCTGG 6001 TTCCCAGCGG TCAAGCCGGG TCACATGATC ACCCATATTA TGAAGAAATG CAGTCAGCTC 6061 CTTAGGGCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCG GTGTTGTCGC TCATGGTAAT 6121 GGCAGCACTA CACAATTCTC TTACCGTCAT GCCATCCGTA AGATGCTTTT CCGTGACCGG 6181 CGAGTACTCA ACCAAGTCGT TTTGTGAGTA GTGTATACGG CGACCAAGCT GCTCTTGCCC 6241 GGCGTCTATA CGGGACAACA CCGCGCCACA TAGCAGTACT TTGAAAGTGC TCATCATCGG 6301 GAATCGTTCT TCGGGGCGGA AAGACTCAAG GATCTTGCCG CTATTGAGAT CCAGTTCGAT 6361 ATAGCCCACT CTTGCACCCA GTTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCGGG 6421 GTGTGCAAAA ACAGGCAAGC AAAATGCCGC AAAGAAGGGA ATGAGTGCGA CACGAAAATG 6481 TTGGATGCTC ATACTCGTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTACTAGTA 6541 CGTCTCTCAA GGATAAGTAA GTAATATTAA GGTACGGGAG GTATTGGACA GGCCGCAATA 6601 AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT GTGTGAATCG ATAGTACTAA 6661 CATACGCTCT CCATCAAAAC AAAACGAAAC AAAACAAACT AGCAAAATAG GCTGTCCCCA 6721 GTGCAAGTGC AGGTGCCAGA ACATTTCTCT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???