pCAGGS-EGFP-Mettl9-TurboID plasmid

PVT108629

pCAGGS-EGFP-Mettl9-TurboID plasmid Information

Plasmid name: pCAGGS-EGFP-Mettl9(mouse)-TurboID
Alias: NM_021554.3;0610012D09Rik; Drev; mMETTL9; MNCb-5680
Gene lenght: 954
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CAG
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCAGGS-EGFP-Mettl9-TurboID plasmid Description

pCAGGS-EGFP-Mettl9-TurboID is a plasmid suitable for protein expression in mammalian cells.

pCAGGS-EGFP-Mettl9-TurboID plasmid Sequence

1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 181 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 361 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA 421 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG 481 CGATGGGGGC GGGGGGGGGG GGGGGGCCCC CCCCAGGCGG GGCGGGGCGG GGCGAGGGGC 541 GGGGCGGGGC GAGGCGGAAA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT 601 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG 661 CGGGAGTCGT TGCGCGCTGC CTTCCCCCCG TGCCCCGCTC CGCCGCCGCC TCGCGCCGCC 721 CGCCCCGGCT CTGACTGACC GCGTTACTCC CACAGGTGAG CGGGCGGGAC GGCCCTTCTC 781 CTCCGGGCTG TAATTAGCGC TTGGTTTAAT GACGGCTTGT TTCTTTTCTG TGGCTGCGTG 841 AAAGCCTTGA GGGGCTCCGG GAGGGCCCTT TGTGCGGGGG GAGCGGCTCG GGGGGTGCGT 901 GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCTCCGC GCTGCCCGGC GGCTGTGAGC 961 GCTGCGGGCG CGGCGCGGGG CTTTGTGCGC TCCGCAGTGT GCGCGAGGGG AGCGCGGCCG 1021 GGGGCGGTGC CCCGCGGTGC GGGGGGGGCT GCGAGGGGAA CAAAGGCTGC GTGCGGGGTG 1081 TGTGCGTGGG GGGGTGAGCA GGGGGTGTGG GCGCGTCGGT CGGGCTGCAA CCCCCCCTGC 1141 ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC CGTACGGGGC 1201 GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG 1261 CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAAGGGGC GCGGCGGCCC CCGGAGCGCC 1321 GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG 1381 GCGCAGGGAC TTCCTTTGTC CCAAATCTGT GCGGAGCCGA AATCTGGGAG GCGCCGCCGC 1441 ACCCCCTCTA GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG 1501 GAGGGCCTTC GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT 1561 CCGCGGGGGG ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT 1621 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG 1681 CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTgccacca 1741 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 1801 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 1861 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 1921 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc 1981 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 2041 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 2101 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 2161 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 2221 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 2281 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 2341 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 2401 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggcg 2461 gtggaggcag tggtggaggc ggtagcGAAT TCatgagact gttggcgggc tggctgtgcc 2521 tgagcctggc gtccgtgtgg ctggcgcgga ggatgtggac gctgcggagc ccgctctccc 2581 gctccctgta cgtgaacatg actagcggcc ccggcgggcc agcggcggcc gcgggcggcg 2641 ggaaggacac gcaccagtgg tatgtgtgca acagagagaa attatgcgaa tcacttcagt 2701 ctgtctttgt tcagagttat cttgaccaag gaacacagat cttcttaaac aacagcattg 2761 agaaatctgg ctggctattt atccaactct atcattcttt tgtatcatct gtttttagcc 2821 tgtttatgtc tagaacatct attaacgggt tgctaggaag aggctccatg tttgtgttct 2881 caccagatca gtttcagaga ctgcttagaa ttaatccgga ctggaaaacc catagacttc 2941 ttgatttagg tgctggagat ggagaagtca cgaaaatcat gagccctcat tttgaagaaa 3001 tttacgccac tgagctttct gaaacaatga tctggcagct ccagaagaag aaatacagag 3061 tgcttggtat aaatgaatgg cagaatacag ggttccagta tgatgtcatc agctgcttaa 3121 atttgctgga tcgctgtgat cagcccctga cattgttaaa agatatcaga agtgtcttgg 3181 agcccaccca aggcagggtc atcctggcat tggttttgcc ctttcatccc tatgtagaaa 3241 acgtaggtgg caagtgggag aaaccatcag aaattctgga aatcaaggga cagaattggg 3301 aagagcaagt gaatagcctg cctgaggtgt tcagaaaagc cggctttgtc gtcgaagctt 3361 tcactagact gccatacctg tgtgaaggcg acatgtacaa tgactactat gttctggacg 3421 atgctgtctt tgttctcaga ccagtaggag gcGGTGGATC AAAAGACAAT ACTGTGCCTC 3481 TGAAGCTGAT CGCTCTCCTG GCTAATGGCG AGTTCCATAG TGGCGAACAG CTGGGAGAAA 3541 CCCTGGGCAT GTCCAGGGCC GCTATCAACA AGCACATTCA GACTCTGCGC GACTGGGGCG 3601 TGGACGTGTT CACCGTGCCC GGAAAGGGCT ACTCTCTGCC CGAGCCTATC CCGCTGCTGA 3661 ACGCTAAACA GATTCTGGGA CAGCTGGACG GCGGGAGCGT GGCAGTCCTG CCTGTGGTCG 3721 ACTCCACCAA TCAGTACCTG CTGGATCGAA TCGGCGAGCT GAAGAGTGGG GATGCTTGCA 3781 TTGCAGAATA TCAGCAGGCA GGGAGAGGAA GCAGAGGGAG GAAATGGTTC TCTCCTTTTG 3841 GAGCTAACCT GTACCTGAGT ATGTTTTGGC GCCTGAAGCG GGGACCAGCA GCAATCGGCC 3901 TGGGCCCGGT CATCGGAATT GTCATGGCAG AAGCGCTGCG AAAGCTGGGA GCAGACAAGG 3961 TGCGAGTCAA ATGGCCCAAT GACCTGTATC TGCAGGATAG AAAGCTGGCA GGCATCCTGG 4021 TGGAGCTGGC CGGAATAACA GGCGATGCTG CACAGATCGT CATTGGCGCC GGGATTAACG 4081 TGGCTATGAG GCGCGTGGAG GAAAGCGTGG TCAATCAGGG CTGGATCACA CTGCAGGAAG 4141 CAGGGATTAA CCTGGACAGG AATACTCTGG CCGCTACGCT GATCCGAGAG CTGCGGGCAG 4201 CCCTGGAACT GTTCGAGCAG GAAGGCCTGG CTCCATATCT GCCACGGTGG GAGAAGCTGG 4261 ATAACTTCAT CAATAGACCC GTGAAGCTGA TCATTGGGGA CAAAGAGATT TTCGGGATTA 4321 GCCGGGGGAT TGATAAACAG GGAGCCCTGC TGCTGGAACA GGACGGAGTT ATCAAACCCT 4381 GGATGGGCGG AGAAATCAGT CTGCGGTCTG CCGAAAAGta GCTCGAGCTA GCAGATCTTT 4441 TTCCCTCTGC CAAAAATTAT GGGGACATCA TGAAGCCCCT TGAGCATCTG ACTTCTGGCT 4501 AATAAAGGAA ATTTATTTTC ATTGCAATAG TGTGTTGGAA TTTTTTGTGT CTCTCACTCG 4561 GAAGGACATA TGGGAGGGCA AATCATTTAA AACATCAGAA TGAGTATTTG GTTTAGAGTT 4621 TGGCAACATA TGCCCATATG CTGGCTGCCA TGAACAAAGG TTGGCTATAA AGAGGTCATC 4681 AGTATATGAA ACAGCCCCCT GCTGTCCATT CCTTATTCCA TAGAAAAGCC TTGACTTGAG 4741 GTTAGATTTT TTTTATATTT TGTTTTGTGT TATTTTTTTC TTTAACATCC CTAAAATTTT 4801 CCTTACATGT TTTACTAGCC AGATTTTTCC TCCTCTCCTG ACTACTCCCA GTCATAGCTG 4861 TCCCTCTTCT CTTATGGAGA TCCCTCGACC TGCAGCCCAA GCTTGGCGTA ATCATGGTCA 4921 TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA 4981 AGCATAAAGT GTAAAGCCTG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 5041 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCGGATCCGC ATCTCAATTA 5101 GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC 5161 CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC 5221 CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG 5281 CAAAAAGCTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA 5341 AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC 5401 AATGTATCTT ATCATGTCTG GATCCGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 5461 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 5521 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 5581 CAGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 5641 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 5701 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 5761 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 5821 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 5881 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 5941 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 6001 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 6061 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 6121 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 6181 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 6241 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 6301 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 6361 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 6421 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 6481 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 6541 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 6601 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 6661 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 6721 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 6781 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 6841 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 6901 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 6961 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 7021 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 7081 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 7141 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 7201 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 7261 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 7321 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 7381 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.