pCAGGS-I-SceI-Trex2 plasmid

PVT111248

pCAGGS-I-SceI-Trex2 plasmid Information

Plasmid name: pCAGGS-I-SceI-Trex2
Alias: NM_011907.4
Gene lenght: 1443
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species:
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CAG
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: β-globin poly(A) signal-F1
Reverse primer:
Copy number: High
Induction:

pCAGGS-I-SceI-Trex2 plasmid Description

pCAGGS-I-SceI-Trex2 It is a plasmid suitable for gene expression in mammalian cells.

pCAGGS-I-SceI-Trex2 plasmid Sequence

1 GTCGAcattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggGtcgag gtgagcccca cgttctgctt cactctcccc 421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc 1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg 1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 1621 gCTCTAGAGc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga aTTCCTCGAA ATTCAGATCC 1741 GCCGCCACTA TGGGATCAAG ATCGCCAAAA AAGAAGAGAA AGGTGCCGAA GAAGCATGca 1801 gcaccaccaa aaaaaaaacg aaaagtagaa gacccacgat ttatgtaccc atacgatgtt 1861 cctgactatg cgggtatgaa aaacatcaaa aaaaaccagg taatgaacct gggtccgaac 1921 tctaaactgc tgaaagaata caaatcccag ctgatcgaac tgaacatcga acagttcgaa 1981 gcaggtatcg gtctgatcct gggtgatgct tacatccgTt ctcgtgatga aggtaaaacc 2041 tactgtatgc agttcgagtg gaaaaacaaa gcatacatgg accacgtatg tctgctgtac 2101 gatcagtggg tactgtcccc gccgcacaaa aaagaacgtg ttaaccacct gggtaacctg 2161 gtaatcacct ggggcgccca gactttcaaa caccaagctt tcaacaaact ggctaacctg 2221 ttcatcgtta acaacaaaaa aaccatcccg aacaacctgg ttgaaaacta cctgaccccg 2281 atgtctctgg catactggtt catggatgat ggtggtaaat gggattacaa caaaaactct 2341 ACCAACAAAT CGATCGTACT GAACACCCAG TCTTTCACTT TCGAAGAAGT AGAATACCTG 2401 GTTAAGGGTC TGCGTAACAA ATTCCAACTG AACTGTTACG TAAAAATCAA CAAAAACAAA 2461 CCGATCATCT ACATCGATTC TATGTCTTAC CTGATCTTCT ACAACCTGAT CAAACCGTAC 2521 CTGATCCCGC AGATGATGTA CAAACTGCCG AACACTATCT CCTCCGAAAC TTTCCTGAAA 2581 AGATCTTGTT CCTGTGAGAA CATCAGCATG TCTGAGCCAC CCCGGGCTGA GACCTTTGTA 2641 TTCCTGGACC TAGAAGCCAC TGGGCTCCCA AACATGGATC CTGAGATTGC AGAGATATCC 2701 CTTTTTGCTG TTCACCGCTC TTCCCTGGAG AACCCAGAAC GGGATGATTC TGGTTCCTTG 2761 GTGCTGCCCC GTGTTCTGGA CAAGCTCACA CTGTGCATGT GCCCGGAGCG CCCCTTTACT 2821 GCCAAGGCCA GTGAGATTAC TGGTTTGAGC AGCGAAAGCC TGATGCACTG CGGGAAGGCT 2881 GGTTTCAATG GCGCTGTGGT AAGGACACTG CAGGGCTTCC TAAGCCGCCA GGAGGGCCCC 2941 ATCTGCCTTG TGGCCCACAA TGGCTTCGAT TATGACTTCC CACTGCTGTG CACGGAGCTA 3001 CAACGTCTGG GTGCCCATCT GCCCCAAGAC ACTGTCTGCC TGGACACACT GCCTGCATTG 3061 CGGGGCCTGG ACCGTGCTCA CAGCCACGGC ACCAGGGCTC AAGGCCGCAA AAGCTACAGC 3121 CTGGCCAGTC TCTTCCACCG CTACTTCCAG GCTGAACCCA GTGCTGCCCA TTCAGCAGAA 3181 GGTGATGTGC ACACCCTGCT TCTGATCTTC CTGCATCGTG CTCCTGAGCT GCTCGCCTGG 3241 GCAGATGAGC AGGCCCGCAG CTGGGCTCAT ATTGAGCCCA TGTACGTGCC ACCTGATGGT 3301 CCAAGCCTCG AAGCCTGAAT GCCAGTGCCA tccattcatc caggctCTCG AGgatctttt 3361 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta 3421 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg 3481 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt 3541 ggcaacatat gccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt 3601 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt 3661 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct 3721 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc 3781 ctcttctctt atgaagatcC CTCGACCTGC AGCCCAAGCT gcggccgcAG CTTGGcgtaa 3841 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 3901 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 3961 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcGGATCCgc 4021 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 4081 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatT TATGcagagg 4141 ccgaggccgc ctcggcctct gAGCTAttcc agaagtagtg aggaggcttt tttggaggcc 4201 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa 4261 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 4321 caaactcatc aatgtatctt atcatgtctg gatcCgctgc attaatgaat cggccaacgc 4381 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 4441 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 4501 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 4561 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 4621 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 4681 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 4741 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaaag ctcacgctgt 4801 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 4861 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 4921 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 4981 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 5041 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 5101 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 5161 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 5221 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 5281 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 5341 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 5401 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 5461 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 5521 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 5581 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 5641 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 5701 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 5761 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 5821 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 5881 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 5941 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 6001 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 6061 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 6121 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 6181 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 6241 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 6301 caaatagggg ttccgcgcac atttccccga aaagtgccac ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.