pcDNA3.1(+)-Flag-AKT1(T308D/S473D) Plasmid
Catalog No. PVTB00033-2f
Vector Backbone pcDNA3.1(+)
Backbone manufacturer Invitrogen
Vector type Mammalian Expression
Backbone size w/o insert 5428bp
Cloning site 5 Kpn I
Site destroyed during cloning NO
Cloning site 3 EcoR I
Bacterial resistance Ampicillin
Growth strain Top10
Growth temperature 37 Centigrade
High or low copy High Copy
Selectable markers Neomycin
Background Mammalian Expression vector of Homo sapiens AKT serine/threonine kinase 1(two points mutation T308D/S473D) with N terminal Flag tag
Gene Information
Alternative Gene Name AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC;RAC-ALPHA
Insert Gene name AKT1(T308D/S473D)
Insert size 1443bp
Accessions NM_005163
Tag Flag(N-term)
Species Homo sapiens (human)
Gene ID 207
Technical Principle & Methodology
The quality assurance and experimental performance of this biological reagent are rigorously optimized and standardized according to its specific technical pathway:
Recombinant DNA Molecular Cloning and Expression Vector Architecture
(重组DNA分子克隆与表达载体工程构建技术). This ensures maximum reproducibility across clinical research and scientific workflows.
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