pCDNA5-CMV-MAP3K11-Hibit-Hyg plasmid

PVT104261

pCDNA5-CMV-MAP3K11-Hibit-Hyg plasmid Information

Plasmid name: pCDNA5-CMV-MAP3K11(human)-Hibit-Hyg
Alias: NM_002419.4;MEKK11; MLK-3; MLK3; PTK1; SPRK
Gene lenght: 2541
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCDNA5-CMV-MAP3K11-Hibit-Hyg plasmid Description

pCDNA5-CMV-MAP3K11-Hibit-Hyg is a plasmid suitable for protein expression in mammalian cells.

pCDNA5-CMV-MAP3K11-Hibit-Hyg plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CCCTATCAGT GATAGAGATC 841 TCCCTATCAG TGATAGAGAT CGTCGACGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA 901 GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA 961 CTCTAGCGTT TAAACTTAAG CTTgccacca tggagccctt gaagagcctc ttcctcaaga 1021 gccctctagg gtcatggaat ggcagtggca gcgggggtgg tgggggcggt ggaggaggcc 1081 ggcctgaggg gtctccaaag gcagcgggtt atgccaaccc ggtgtggaca gccctgttcg 1141 actacgagcc cagtgggcag gatgagctgg ccctgaggaa gggtgaccgt gtggaggtgc 1201 tgtcccggga cgcagccatc tcaggagacg agggctggtg ggcgggccag gtgggtggcc 1261 aggtgggcat cttcccgtcc aactatgtgt ctcggggtgg cggcccgccc ccctgcgagg 1321 tggccagctt ccaggagctg cggctggagg aggtgatcgg cattggaggc tttggcaagg 1381 tgtacagggg cagctggcga ggtgagctgg tggctgtgaa ggcagctcgc caggaccccg 1441 atgaggacat cagtgtgaca gccgagagcg ttcgccagga ggcccggctc ttcgccatgc 1501 tggcacaccc caacatcatt gccctcaagg ctgtgtgcct ggaggagccc aacctgtgcc 1561 tggtgatgga gtatgcagcc ggtgggcccc tcagccgagc tctggccggg cggcgcgtgc 1621 ctccccatgt gctggtcaac tgggctgtgc agattgcccg tgggatgcac tacctgcact 1681 gcgaggccct ggtgcccgtc atccaccgtg atctcaagtc caacaacatt ttgctgctgc 1741 agcccattga gagtgacgac atggagcaca agaccctgaa gatcaccgac tttggcctgg 1801 cccgagagtg gcacaaaacc acacaaatga gtgccgcggg cacctacgcc tggatggctc 1861 ctgaggttat caaggcctcc accttctcta agggcagtga cgtctggagt tttggggtgc 1921 tgctgtggga actgctgacc ggggaggtgc cataccgtgg cattgactgc cttgctgtgg 1981 cctatggcgt agctgttaac aagctcacac tgcccatccc atccacctgc cccgagccct 2041 tcgcacagct tatggccgac tgctgggcgc aggaccccca ccgcaggccc gacttcgcct 2101 ccatcctgca gcagttggag gcgctggagg cacaggtcct acgggaaatg ccgcgggact 2161 ccttccattc catgcaggaa ggctggaagc gcgagatcca gggtctcttc gacgagctgc 2221 gagccaagga aaaggaacta ctgagccgcg aggaggagct gacgcgagcg gcgcgcgagc 2281 agcggtcaca ggcggagcag ctgcggcggc gcgagcacct gctggcccag tgggagctag 2341 aggtgttcga gcgcgagctg acgctgctgc tgcagcaggt ggaccgcgag cgaccgcacg 2401 tgcgccgccg ccgcgggaca ttcaagcgca gcaagctccg ggcgcgcgac ggcggcgagc 2461 gtatcagcat gccactcgac ttcaagcacc gcatcaccgt gcaggcctca cccggccttg 2521 accggaggag aaacgtcttc gaggtcgggc ctggggattc gcccaccttt ccccggttcc 2581 gagccatcca gttggagcct gcagagccag gccaggcatg gggccgccag tccccccgac 2641 gtctggagga ctcaagcaat ggagagcggc gagcatgctg ggcttggggt cccagttccc 2701 ccaagcctgg ggaagcccag aatgggagga gaaggtcccg catggacgaa gccacatggt 2761 acctggattc agatgactca tcccccttag gatctccttc cacaccccca gcactcaatg 2821 gtaacccccc gcggcctagc ctggagcccg aggagcccaa gaggcctgtc cccgcagagc 2881 gcggtagcag ctctgggacg cccaagctga tccagcgggc gctgctgcgc ggcaccgccc 2941 tgctcgcctc gctgggcctt ggccgcgacc tgcagccgcc gggaggccca ggacgcgagc 3001 gcggggagtc cccgacaaca ccccccacgc caacgcccgc gccctgcccg accgagccgc 3061 ccccttcccc gctcatctgc ttctcgctca agacgcccga ctccccgccc actcctgcac 3121 ccctgttgct ggacctgggt atccctgtgg gccagcggtc agccaagagc ccccgacgtg 3181 aggaggagcc ccgcggaggc actgtctcac ccccaccggg gacatcacgc tctgctcctg 3241 gcaccccagg caccccacgt tcaccacccc tgggcctcat cagccgacct cggccctcgc 3301 cccttcgcag ccgcattgat ccctggagct ttgtgtcagc tgggccacgg ccttctcccc 3361 tgccatcacc acagcctgca ccccgccgag caccctggac cttgttcccg gactcagacc 3421 ccttctggga ctccccacct gccaacccct tccagggggg cccccaggac tgcagggcac 3481 agaccaaaga catgggtgcc caggccccgt gggtgccgga agcggggcct GTGAGCGGCT 3541 GGCGGCTGTT CAAGAAGATT AGCTGAGGAT CCACTAGTCC AGTGTGGTGG AATTCTGCAG 3601 ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC GTTTAAACCC GCTGATCAGC 3661 CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT 3721 GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA 3781 TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA 3841 GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC 3901 GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG 3961 CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC 4021 CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC 4081 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA 4141 AAAACTTGAT TAGGGTGATG GTTCACGTAC CTAGAAGTTC CTATTCCGAA GTTCCTATTC 4201 TCTAGAAAGT ATAGGAACTT CCTTGGCCAA AAAGCCTGAA CTCACCGCGA CGTCTGTCGA 4261 GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG ATGCAGCTCT CGGAGGGCGA 4321 AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA TATGTCCTGC GGGTAAATAG 4381 CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTTATCGG CACTTTGCAT CGGCCGCGCT 4441 CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG AGCCTGACCT ATTGCATCTC 4501 CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA ACCGAACTGC CCGCTGTTCT 4561 GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC GATCTTAGCC AGACGAGCGG 4621 GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT ACATGGCGTG ATTTCATATG 4681 CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG ATGGACGACA CCGTCAGTGC 4741 GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC GAGGACTGCC CCGAAGTCCG 4801 GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG ACGGACAATG GCCGCATAAC 4861 AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGATTCC CAATACGAGG TCGCCAACAT 4921 CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT GGAGCAGCAG ACGCGCTACT TCGAGCGGAG 4981 GCATCCGGAG CTTGCAGGAT CGCCGCGGCT CCGGGCGTAT ATGCTCCGCA TTGGTCTTGA 5041 CCAACTCTAT CAGAGCTTGG TTGACGGCAA TTTCGATGAT GCAGCTTGGG CGCAGGGTCG 5101 ATGCGACGCA ATCGTCCGAT CCGGAGCCGG GACTGTCGGG CGTACACAAA TCGCCCGCAG 5161 AAGCGCGGCC GTCTGGACCG ATGGCTGTGT AGAAGTACTC GCCGATAGTG GAAACCGACG 5221 CCCCAGCACT CGTCCGAGGG CAAAGGAATA GCACGTACTA CGAGATTTCG ATTCCACCGC 5281 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT 5341 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA 5401 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 5461 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC 5521 GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 5581 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 5641 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 5701 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 5761 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 5821 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 5881 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 5941 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 6001 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 6061 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 6121 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 6181 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 6241 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 6301 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 6361 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 6421 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 6481 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 6541 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 6601 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 6661 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 6721 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 6781 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 6841 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 6901 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 6961 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 7021 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 7081 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 7141 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 7201 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 7261 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 7321 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 7381 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 7441 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 7501 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 7561 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 7621 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 7681 ATTTCCCCGA AAAGTGCCAC CTGACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???