pCDNA5-CMV-ZNF217-Hibit-Hyg plasmid

PVT110507

pCDNA5-CMV-ZNF217-Hibit-Hyg plasmid Information

Plasmid name: pCDNA5-CMV-ZNF217(human)-Hibit-Hyg
Alias: NM_006526.3;ZABC1
Gene lenght: 3144
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCDNA5-CMV-ZNF217-Hibit-Hyg plasmid Description

pCDNA5-CMV-ZNF217-Hibit-Hyg It is a plasmid suitable for gene expression in mammalian cells.

pCDNA5-CMV-ZNF217-Hibit-Hyg plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CCCTATCAGT GATAGAGATC 841 TCCCTATCAG TGATAGAGAT CGTCGACGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA 901 GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA 961 CTCTAGCGTT TAAACTTAAG CTTgccacca tgcaatcgaa agtgacagga aacatgccaa 1021 ctcaatccct cttaatgtac atggatgggc cagaagtgat tggcagctct cttggcagtc 1081 cgatggagat ggaggatgcc ttgtcaatga aagggaccgc tgttgttcca ttccgagcta 1141 cacaagaaaa aaatgtcatc caaatcgagg ggtatatgcc cttggattgc atgttctgca 1201 gccagacctt cacacattca gaagacctta ataaacatgt cttaatgcaa caccggccta 1261 ccctctgtga accagcagtt cttcgggttg aagcagagta tctcagtccg cttgataaaa 1321 gtcaagtgcg aacagaacct cccaaggaaa agaattgcaa ggaaaatgaa tttagctgtg 1381 aggtatgtgg gcagacattt agagtcgctt ttgatgttga gatccacatg agaacacaca 1441 aagattcttt cacttacggg tgtaacatgt gcggaagaag attcaaggag ccttggtttc 1501 ttaaaaatca catgcggaca cataatggca aatcgggggc cagaagcaaa ctgcagcaag 1561 gcttggagag tagtccagca acgatcaacg aggtcgtcca ggtgcacgcg gccgagagca 1621 tctcctctcc ttacaaaatc tgcatggttt gtggcttcct atttccaaat aaagaaagtc 1681 taattgagca ccgcaaggtg cacaccaaaa aaactgcttt cggtaccagc agcgcgcaga 1741 cagactctcc acaaggagga atgccgtcct cgagggagga cttcctgcag ttgttcaact 1801 tgagaccaaa atctcaccct gaaacgggga agaagcctgt cagatgcatc cctcagctcg 1861 atccgttcac caccttccag gcttggcagc tggctaccaa aggaaaagtt gccatttgcc 1921 aagaagtgaa ggaatcgggg caagaaggga gcaccgacaa cgacgattcg agttccgaga 1981 aggagcttgg agaaacaaat aagggcagtt gtgcaggcct ctcgcaagag aaagagaagt 2041 gcaaacactc ccacggcgaa gcgccctccg tggacgcgga tcccaagtta cccagtagca 2101 aggagaagcc cactcactgc tccgagtgcg gcaaagcttt cagaacctac caccagctgg 2161 tcttgcactc cagggtccac aagaaggacc ggagggccgg cgcggagtcg cccaccatgt 2221 ctgtggacgg gaggcagccg gggacgtgtt ctcctgacct cgccgcccct ctggatgaaa 2281 atggagccgt ggatcgaggg gaaggtggtt ctgaagacgg atctgaggat gggcttcccg 2341 aaggaatcca tctggataaa aatgatgatg gaggaaaaat aaaacatctt acatcttcaa 2401 gagagtgtag ttattgtgga aagtttttcc gttcaaatta ttacctcaat attcatctca 2461 gaacgcatac aggtgaaaaa ccatacaaat gtgaattttg tgaatatgct gcagcccaga 2521 agacatctct gaggtatcac ttggagagac atcacaagga aaaacaaacc gatgttgctg 2581 ctgaagtcaa gaacgatggt aaaaatcagg acactgaaga tgcactatta accgctgaca 2641 gtgcgcaaac caaaaatttg aaaagatttt ttgatggtgc caaagatgtt acaggcagtc 2701 cacctgcaaa gcagcttaag gagatgcctt ctgtttttca gaatgttctg ggcagcgctg 2761 tcctctcacc agcacacaaa gatactcagg atttccataa aaatgcagct gatgacagtg 2821 ctgataaagt gaataaaaac cctacccctg cttacctgga cctgttaaaa aagagatcag 2881 cagttgaaac tcaggcaaat aacctcatct gtagaaccaa ggcggatgtt actcctcctc 2941 cggatggcag taccacccat aaccttgaag ttagccccaa agagaagcaa acggagaccg 3001 cagctgactg cagatacagg ccaagtgtgg attgtcacga aaaaccttta aatttatccg 3061 tgggggctct tcacaattgc ccggcaattt ctttgagtaa aagtttgatt ccaagtatca 3121 cctgtccatt ttgtaccttc aagacatttt atccagaagt tttaatgatg caccagagac 3181 tggagcataa atacaatcct gacgttcata aaaactgtcg aaacaagtcc ttgcttagaa 3241 gtcgacgtac cggatgcccg ccagcgttgc tgggaaaaga tgtgcctccc ctctctagtt 3301 tctgtaaacc caagcccaag tctgctttcc cggcgcagtc caaatccctg ccatctgcga 3361 aggggaagca gagccctcct gggccaggca aggcccctct gacttcaggg atagactcta 3421 gcactttagc cccaagtaac ctgaagtccc acagaccaca gcagaatgtg ggggtccaag 3481 gggccgccac caggcaacag caatctgaga tgtttcctaa aaccagtgtt tcccctgcac 3541 cggataagac aaaaagaccc gagacaaaat tgaaacctct tccagtagct ccttctcagc 3601 ccaccctcgg cagcagtaac atcaatggtt ccatcgacta ccccgccaag aacgacagcc 3661 cgtgggcacc tccgggaaga gactatttct gtaatcggag tgccagcaat actgcagcag 3721 aatttggtga gccccttcca aaaagactga agtccagcgt ggttgccctt gacgttgacc 3781 agcccggggc caattacaga agaggctatg accttcccaa gtaccatatg gtcagaggca 3841 tcacatcact gttaccgcag gactgtgtgt atccgtcgca ggcgctgcct cccaaaccaa 3901 ggttcctgag ctccagcgag gtcgattctc caaatgtgct gactgttcag aagccctatg 3961 gtggctccgg gccactttac acttgtgtgc ctgctggtag tccagcatcc agctcgacgt 4021 tagaaggaaa aaggcctgtg tcatatcaac acttatctaa cagcatggca caaaagagaa 4081 actatgagaa ttttattggg aatgcacatt atcgaccaaa tgacaaaaaa actGTGAGCG 4141 GCTGGCGGCT GTTCAAGAAG ATTAGCTGAG GATCCACTAG TCCAGTGTGG TGGAATTCTG 4201 CAGATATCCA GCACAGTGGC GGCCGCTCGA GTCTAGAGGG CCCGTTTAAA CCCGCTGATC 4261 AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC 4321 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC 4381 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG 4441 GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA 4501 GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT 4561 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC 4621 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA 4681 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC 4741 CAAAAAACTT GATTAGGGTG ATGGTTCACG TACCTAGAAG TTCCTATTCC GAAGTTCCTA 4801 TTCTCTAGAA AGTATAGGAA CTTCCTTGGC CAAAAAGCCT GAACTCACCG CGACGTCTGT 4861 CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC TCTCGGAGGG 4921 CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC TGCGGGTAAA 4981 TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG CATCGGCCGC 5041 GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC GAGAGCCTGA CCTATTGCAT 5101 CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT GAAACCGAAC TGCCCGCTGT 5161 TCTGCAGCCG GTCGCGGAGG CCATGGATGC GATCGCTGCG GCCGATCTTA GCCAGACGAG 5221 CGGGTTCGGC CCATTCGGAC CGCAAGGAAT CGGTCAATAC ACTACATGGC GTGATTTCAT 5281 ATGCGCGATT GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGACG ACACCGTCAG 5341 TGCGTCCGTC GCGCAGGCTC TCGATGAGCT GATGCTTTGG GCCGAGGACT GCCCCGAAGT 5401 CCGGCACCTC GTGCACGCGG ATTTCGGCTC CAACAATGTC CTGACGGACA ATGGCCGCAT 5461 AACAGCGGTC ATTGACTGGA GCGAGGCGAT GTTCGGGGAT TCCCAATACG AGGTCGCCAA 5521 CATCTTCTTC TGGAGGCCGT GGTTGGCTTG TATGGAGCAG CAGACGCGCT ACTTCGAGCG 5581 GAGGCATCCG GAGCTTGCAG GATCGCCGCG GCTCCGGGCG TATATGCTCC GCATTGGTCT 5641 TGACCAACTC TATCAGAGCT TGGTTGACGG CAATTTCGAT GATGCAGCTT GGGCGCAGGG 5701 TCGATGCGAC GCAATCGTCC GATCCGGAGC CGGGACTGTC GGGCGTACAC AAATCGCCCG 5761 CAGAAGCGCG GCCGTCTGGA CCGATGGCTG TGTAGAAGTA CTCGCCGATA GTGGAAACCG 5821 ACGCCCCAGC ACTCGTCCGA GGGCAAAGGA ATAGCACGTA CTACGAGATT TCGATTCCAC 5881 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT 5941 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC 6001 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 6061 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC 6121 GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 6181 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 6241 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 6301 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 6361 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 6421 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 6481 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 6541 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 6601 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 6661 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 6721 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 6781 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 6841 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 6901 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 6961 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 7021 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 7081 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 7141 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 7201 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 7261 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 7321 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 7381 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 7441 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 7501 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 7561 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 7621 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 7681 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 7741 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 7801 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 7861 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 7921 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 7981 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 8041 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 8101 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 8161 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 8221 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 8281 CACATTTCCC CGAAAAGTGC CACCTGACGT C

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.