pCE27 Plasmid

Alias:
Gene length:
Host: E.coli
Use(s): Gene editing
Fragment Type: CRISPR
Fragmented species:
Prokaryotic resistance: Amp
Screening Markers: NAT
Fluorescent labeling:
Promoter:
replicon: pBR322
Copy Number:
Competent cells: DH5a
Temperature: 37 Degrees
Back Bones:
Forward primer:
Reverse primer: Induction method:
Sequence:
atatatgagtaaacttggtctgacagttaccaatgcttgatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttgacggcacggccacgcgtttaaaccgccaggtgatgctgaagctattgaagctttggatggttctttcactaccgatactgtttttagagttactgctactggtgatggtttcactttgagagaagttccagttgatccaccattgactaaggttttcccagatgatgaatccgatgatgaatccgatgctggtgaagatggtgatccagattctagaactttcgttgcttatggtgatgatggtgatttggctggtttcgttgttgtttcttattctggttggaacagaagattgactgttgaagatattgaagttgctccagaacatagaggtcatggtgttggtagagctttgatgggtttggctactgaattcgccagagaaagaggtgctggtcatttgtggttggaagttaccaatgttaatgctccagctattcatgcttatagaagaatgggtttcactttgtgtggtttggatactgctttatacgatggtactgcttccgatggtgaacaagctttgtatatgtctatgccatgtccataataactcgagtcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaaagcttaacttttagagtcacttaccctacaaaggtgtaagcttccctcagatcaggcggcacgcatctgatatctaacatccattgtaaaattagtgaagattaacactcagagccagttattttgaacacagatggcttttggctgctcttatcgtctacgtttcttttttttttttttttttttttttttcctaattttttacgagttcactaattggtttcgggggctcaattttcgaatgcgacattcataaactttgaccttttatgtgttgatctggataattttgacctcaaaactcggaaaaattgatgtatagcgtattcgacatggctttaaaagtcgcagagttctacgattttcatttgcactggcccatccggtaagcctcggggctccggcatgtgagggttcgactccctcagcagaaaacatgccgtgaaagaaaacaccagttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttctcgagtttttttatcgagtctccattgaaggccagtgttgtcaaaggaaccaatttcaccgtggtgcgtgaattggtcggtggtatctactttggtgagagacaagaacaggaagagtctgccgacggtcagagcgcttgggacaccgagaagtacagtgtctctgaagttgaaagaatcaccagaatggctgccttcatggctttgcagcacaaccctcctttgcccatctggtccttagacaaggccaacgtgcttgcctcatctagattgtggagaaagacggtcgacaaggtgatgagcgaggagttccctcaattgaccatcaaccaccagctcattgactctgctgccatgattttggtgcagagcccaactaaattgaacggtgttgtgatcacgtcaaacatgtttggtgatattatttccgacgaggcttccgtgatccctggctccttgggtcttttaccttctgcatctttggcttcattaccagatacaaataaggcgtttggtttgtacgaaccatgccacggttcggctccggacttgccagaaaacaaggtcaaccctgtcgccacgattttgtcggccgctatgatgttgcgcttgtcattagactgcctcagagaggcagaggccttggagaaggctgttcgtaacgttttggactctggtattcgaaccgcagatttgaagggctcaagttccactcaggaagtgggcgatgccattgctgctgaggtaaagaagcttttgcagaaataacggtgtttaaaccccagcgcctggcgggcgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagt
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Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
4.;