pCMV-3×Myc-TJP2-Neo plasmid

PVT106967

pCMV-3×Myc-TJP2-Neo plasmid Information

Plasmid name: pCMV-3×Myc-TJP2(human)-Neo
Alias: NM_004817.4;C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;PFIC4; X104; ZO2
Gene lenght: 3573
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×Myc-TJP2-Neo plasmid Description

pCMV-3×Myc-TJP2-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×Myc-TJP2-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttAAACTT AAGCCACCAT GGCGGCGGAA CAAAAACTAA TATCAGAGGA AGATTTGGAA 961 CAGAAACTAA TCAGTGAAGA AGATTTAGAG CAAAAGTTAA TTTCCGAGGA GGACTTATCA 1021 GCGGAAAAGC TTatgccggt gcgaggagac cgcgggtttc caccccggcg ggagctgtca 1081 ggttggctcc gcgccccagg catggaagag ctgatatggg aacagtacac tgtgacccta 1141 caaaaggatt ccaaaagagg atttggaatt gcagtgtccg gaggcagaga caacccccac 1201 tttgaaaatg gagaaacgtc aattgtcatt tctgatgtgc tcccgggtgg gcctgctgat 1261 gggctgctcc aagaaaatga cagagtggtc atggtcaatg gcacccccat ggaggatgtg 1321 cttcattcgt ttgcagttca gcagctcaga aaaagtggga aggtcgctgc tattgtggtc 1381 aagaggcccc ggaaggtcca ggtggccgca cttcaggcca gccctcccct ggatcaggat 1441 gaccgggctt ttgaggtgat ggacgagttt gatggcagaa gtttccggag tggctacagc 1501 gagaggagcc ggctgaacag ccatgggggg cgcagccgca gctgggagga cagcccggaa 1561 agggggcgtc cccatgagcg ggcccggagc cgggagcggg acctcagccg ggaccggagc 1621 cgtggccgga gcctggagcg gggcctggac caagaccatg cgcgcacccg agaccgcagc 1681 cgtggccgga gcctggagcg gggcctggac cacgactttg ggccatcccg ggaccgggac 1741 cgtgaccgca gccgcggccg gagcattgac caggactacg agcgagccta tcaccgggcc 1801 tacgacccag actacgagcg ggcctacagc ccggagtaca ggcgcggggc ccgccacgat 1861 gcccgctctc ggggaccccg aagccgcagc cgcgagcacc cgcactcacg gagccccagc 1921 cccgagccta gggggcggcc ggggcccatc ggggtcctcc tgatgaaaag cagagcgaac 1981 gaagagtatg gtctccggct tgggagtcag atcttcgtaa aggaaatgac ccgaacgggt 2041 ctggcaacta aagatggcaa ccttcacgaa ggagacataa ttctcaagat caatgggact 2101 gtaactgaga acatgtcttt aacggatgct cgaaaattga tagaaaagtc aagaggaaaa 2161 ctacagctag tggtgttgag agacagccag cagaccctca tcaacatccc gtcattaaat 2221 gacagtgact cagaaataga agatatttca gaaatagagt caaaccgatc attttctcca 2281 gaggagagac gtcatcagta ttctgattat gattatcatt cctcaagtga gaagctgaag 2341 gaaaggccaa gttccagaga ggacacgccg agcagattgt ccaggatggg tgcgacaccc 2401 actcccttta agtccacagg ggatattgca ggcacagttg tcccagagac caacaaggaa 2461 cccagatacc aagaggaccc cccagctcct caaccaaaag cagccccgag aacttttctt 2521 cgtcctagtc ctgaagatga agcaatatat ggccctaata ccaaaatggt aaggttcaag 2581 aagggagaca gcgtgggcct ccggttggct ggtggcaatg atgtcgggat atttgttgct 2641 ggcattcaag aagggacctc ggcggagcag gagggccttc aagaaggaga ccagattctg 2701 aaggtgaaca cacaggattt cagaggatta gtgcgggagg atgccgttct ctacctgtta 2761 gaaatcccta aaggtgaaat ggtgaccatt ttagctcaga gccgagccga tgtgtataga 2821 gacatcctgg cttgtggcag aggggattcg ttttttataa gaagccactt tgaatgtgag 2881 aaggaaactc cacagagcct ggccttcacc agaggggagg tcttccgagt ggtagacaca 2941 ctgtatgacg gcaagctggg caactggctg gctgtgagga ttgggaacga gttggagaaa 3001 ggcttaatcc ccaacaagag cagagctgaa caaatggcca gtgttcaaaa tgcccagaga 3061 gacaacgctg gggaccgggc agatttctgg agaatgcgtg gccagaggtc tggggtgaag 3121 aagaacctga ggaaaagtcg ggaagacctc acagctgttg tgtctgtcag caccaagttc 3181 ccagcttatg agagggtttt gctgcgagaa gctggtttca agagacctgt ggtcttattc 3241 ggccccatag ctgatatagc aatggaaaaa ttggctaatg agttacctga ctggtttcaa 3301 actgctaaaa cggaaccaaa agatgcagga tctgagaaat ccactggagt ggtccggtta 3361 aataccgtga ggcaaattat tgaacaggat aagcatgcac tactggatgt gactccgaaa 3421 gctgtggacc tgttgaatta cacccagtgg ttcccaattg tgattttttt caacccagac 3481 tccagacaag gtgtcaaaac catgagacaa aggttaaatc caacgtccaa caaaagttct 3541 cgaaagttat ttgatcaagc caacaagctt aaaaaaacgt gtgcacacct ttttacagct 3601 acaatcaacc taaattcagc caatgatagc tggtttggca gcttaaagga cactattcag 3661 catcagcaag gagaagcggt ttgggtctct gaaggaaaga tggaagggat ggatgatgac 3721 cccgaagacc gcatgtccta cttaaccgcc atgggcgcgg actatctgag ttgcgacagc 3781 cgcctcatca gtgactttga agacacggac ggtgaaggag gcgcctacac tgacaatgag 3841 ctggatgagc cagccgagga gccgctggtg tcgtccatca cccgctcctc ggagccggtg 3901 cagcacgagg agagcataag gaaacccagc ccagagccac gagctcagat gaggagggct 3961 gctagcagcg atcaacttag ggacaatagc ccgcccccag cattcaagcc agagccgccc 4021 aaggccaaaa cccagaacaa agaagaatcc tatgacttct ccaaatccta tgaatataag 4081 tcaaacccct ctgccgttgc tggtaatgaa actcctgggg catctaccaa aggttatcct 4141 cctcctgttg cagcaaaacc tacctttggg cggtctatac tgaagccctc cactcccatc 4201 cctcctcaag agggtgagga ggtgggagag agcagtgagg agcaagataa tgctcccaaa 4261 tcagtcctgg gcaaagtcaa aatatttgag aagatggatc acaaggccag gttacagaga 4321 atgcaggagc tccaggaagc acagaatgca aggatcgaaa ttgcccagaa gcatcctgat 4381 atctatgcag ttccaatcaa aacgcacaag ccagaccctg gcacgcccca gcacacgagt 4441 tccagacccc ctgagccaca gaaagctcct tccagacctt atcaggatac cagaggaagt 4501 tatggcagtg atgccgagga ggaggagtac cgccagcagc tgtcagaaca ctccaagcgc 4561 ggttactatg gccagtctgc ccgataccgg gacacagaat tataggaatt ctgcagatat 4621 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg 4681 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 4741 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 4801 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 4861 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 4921 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 4981 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 5041 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 5101 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 5161 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 5221 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 5281 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 5341 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 5401 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 5461 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 5521 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 5581 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 5641 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 5701 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 5761 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 5821 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 5881 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 5941 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 6001 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 6061 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 6121 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 6181 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 6241 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 6301 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 6361 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 6421 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 6481 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 6541 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 6601 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 6661 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 6721 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 6781 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6841 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 6901 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 6961 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 7021 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 7081 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 7141 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 7201 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 7261 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 7321 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 7381 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 7441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 7501 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 7561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 7621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 7681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 7741 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 7801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7861 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 7921 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 7981 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 8041 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 8101 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 8161 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 8221 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 8281 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 8341 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 8401 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 8461 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 8521 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 8581 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 8641 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 8701 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 8761 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 8821 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 8881 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 8941 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 9001 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 9061 ccgaaaagtg ccacctgacg tc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???