pCMV-6×His-Atp1a4-3×HA-Neo plasmid

PVT109614

pCMV-6×His-Atp1a4-3×HA-Neo plasmid Information

Plasmid name: pCMV-6×His-Atp1a4(mouse)-3×HA-Neo
Alias: NM_013734.1
Gene lenght: 3096
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-6×His-Atp1a4-3×HA-Neo plasmid Description

pCMV-6×His-Atp1a4-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-6×His-Atp1a4-3×HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCGCCACC ATGCACCATC ACCATCACCA Tatggagcct 961 ggaaaagaaa aggaggtaga ggcccctggt gagctgaatc agaaaccaag acccagcacc 1021 aggtcctcaa ctacaaatag acagccgaaa atgaaaagaa ggaagaaaga tttagaagag 1081 ctgaagaagg aagtggttat ggatgatcac aagctaacgt tggatgagct gagtgccaag 1141 tactctgtgg acttgaccaa gggtcttagc atcctagaag cccaggatat tctgtttcaa 1201 aatggaccga atgtactaac tcctcctcca accactccag agtgggtcaa attctgtaga 1261 caactgtttg gtggcttctc cctcctactg tggactggtg cctgtctctg cttcctggcc 1321 tatggcatac atgtaaatta ttacaaagaa aacgccaaca aagataatct gtacctgggg 1381 attgtactat ctgccgtggt catcattacc ggctgcttct catattacca ggaggccaag 1441 agctccaaga tcatggagtc ttttaagaac atggtgcctc agcaagctct ggtgattcga 1501 gatggagaga aaatgcaaat caatgtaaga gatgtggttt tgggagacct agtagaggtg 1561 aagggaggag accaaatccc tgctgatatc agggttatct cggcgcaagg atgcaaggta 1621 gacaattcct ccttgactgg ggagtcagaa ccccagtctc gctgccctga ttgcactcat 1681 gagaaccctc tggagacacg gaatatcatc ttcttctcca ctaactgtgt agaaggaaca 1741 gctcggggta ttgtgattgc tacaggagac tacacagtaa tgggccggat tgcttccctg 1801 acatcaggct tgcaaatggg caagaccccg attgccaccg aaatcgaaca cttcatccat 1861 ttgatcacag ctgtcgcggt cttcctcggt gtttcatttt tttggctctc gattatcttg 1921 ggttacacct ggctggatgc tgtcattttc cttattggta tcattgtggc caatgtgccg 1981 gaagggctgc tggccactgt cactgtgtgc ctgacgctga ccgccaagcg catggctcgc 2041 aagaactgcc tggtgaagaa cctggaggcc gtggagacgc tgggctccac gtccaccatc 2101 tgctcagaca agacgggcac cctcacccag aaccgcatga cagtggcgca tttgtggttt 2161 gataagactg tgtatgaggc cgacaccagt gaggagcaaa ccacggggaa aacgtttccc 2221 aagtcctcag acacctggtt ttacctggct cgaatcgctg gcctctgtaa ccgggccgac 2281 tttaaacccc atcaggagtc tgttcccata gctaagcggg caacaacagg ggatgcttcc 2341 gagtcagctc tcctcaaatt cattgagcag tcttacaacc ccgtgagcga aatgaggcag 2401 aaaaacccca aggtggcgga gattcccttt aactccacca acaagtatca gatgtccatc 2461 caccttctgg aagataattc cgaagcccac gtgctgctaa tgaaaggtgc cccagagagg 2521 atctttgatt tttgctccag tttcctgctg aatgggcagg agtaccctat ggatgaggaa 2581 atgaagatgg acttccagaa tgcctacata gaactgggag gtctgggcga acgtgtgctg 2641 gggttctgct tcttgaactt gcctagcaat ttctctaagg ggttccagtt taataccgat 2701 gagttaaatt ttcccatgga aaatctgtgt ttcgcgggcc tcatctccat gattgatcct 2761 cctcggacgg ccgtgcctga cgctgtgagc aaatgtcgaa gcgcggggat caaggtgatc 2821 atggtaacgg gggatcatcc catcacagcc aaagctattg ccaaaagtgt gggtatcatt 2881 tcagaaggta acgacacggc ggaggacatt gccgctcggc tgaatatccc tatcagtcaa 2941 gtcaataaca agtctgttaa agccattgta gtgcacggct cagaactgaa agacatggaa 3001 tcccaacagc tggacgacat cctcaagtcc tataaggaga ttgtctttgc ccggacctct 3061 cctcagcaga aactcatcat tgtggagggg tgtcagcgac tgggagccat tgtggcagtg 3121 actggtgacg gggtgaacga ctcccccgca ctgaagaagg ctgacatcgg cattgccatg 3181 ggcatcacag gctctgatgt ctctaaacag gcggctgaca tgatccttct tgacgacaat 3241 tttgcctcca ttgtgacagg cgtggaggag ggccgcctca tctttgacaa cctgaagaag 3301 tccatcgcat acaccctgac cagcaacatc cctgagatca cccccttcct gctgttcatc 3361 atcttaagca tacccttgcc tctgggcacc ataaccattc tctgcatcga cctgggcact 3421 gacatggtcc ctgctatctc cctggcttat gaatcacctg aaagtgacat catgaaaagg 3481 cttccacgga accccaaaac ggacaatctt gtgaacaacc gtcttattgg catggcctat 3541 gggcagattg ggatgataca ggctctggct ggatttttca cctattttgt gatcctggcg 3601 gagaatggtt ttaagcctct tgatcttctg ggtatccgtc tatactggga tgatacacaa 3661 ttaaatgacc tagaggacag ctatgggcag cagtggacct atgagcagcg gaaggtggtg 3721 gaattcacat gccaaacggc cttcttcatc agtatcgtga ttgtgcagtg ggctgacctc 3781 atcatctgca agacacgcag aaattcactc ttcaagcagg gcatgaaaaa caaaatccta 3841 atatttggac tccttgagga gacagtcctg gcagctttcc tgtcctacgt tccaggcatg 3901 gatgtgtccc tgcggatgta cccactcaag ataaactggt ggttctgcgc acttccctac 3961 agtgttctca tcttcgtcta tgatgaaatc agaaaactta tcatccggag acggcctggt 4021 ggatggctgg agaaggagac gtactacCTC GAGTACCCAT ACGACGTACC TGATTATGCA 4081 ACTTATCCTT ATGATGTACC TGATTATGCT AGCTATCCTT ATGATGTCCC AGACTACGCA 4141 tgaGGGCCCG TTTAAACCCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT 4201 GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT 4261 TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG 4321 GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG 4381 GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT 4441 CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 4501 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 4561 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 4621 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 4681 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 4741 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 4801 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 4861 TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT 4921 CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA 4981 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA 5041 CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT 5101 CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT 5161 CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC 5221 TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC 5281 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT 5341 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT 5401 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC 5461 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG 5521 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC 5581 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA 5641 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC 5701 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG 5761 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG 5821 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA 5881 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG 5941 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT 6001 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC 6061 TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA 6121 CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT 6181 CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT 6241 CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC 6301 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT 6361 CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG 6421 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC 6481 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC 6541 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT 6601 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC 6661 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 6721 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 6781 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 6841 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 6901 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 6961 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 7021 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 7081 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 7141 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 7201 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 7261 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 7321 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA 7381 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 7441 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 7501 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 7561 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 7621 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA 7681 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC 7741 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC 7801 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC 7861 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 7921 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC 7981 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 8041 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC 8101 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG 8161 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 8221 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT 8281 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 8341 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA 8401 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA 8461 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 8521 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG 8581 ATCGGGAGAT CTCCCGATCC CCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.