pCMV-Anks1-3×FLAG-Neo plasmid

PVT104540

pCMV-Anks1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Anks1(mouse)-3×FLAG-Neo
Alias: NM_001286040.1;Anks1a; mKIAA0229;
Gene lenght: 3567
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Anks1-3×FLAG-Neo plasmid Description

pCMV-Anks1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Anks1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatggg gaaggagcag gagctgctgg 781 aggcggcccg caccgggcac ctcccggccg tggagaagct gctgtccggg aagcggctct 841 cctccggctt cggcggcggc ggcggcggct caggcagcgg cggcggctcc gggggcggcg 901 gcctcggttc ttctagccac ccgctctcca gtctgctgag catgtggaga gggccgaacg 961 tgaactgtgt agatagcacc ggctacacgc cactacacca cgctgctttg aatggacaca 1021 gggatgtggt cgaggtcctt ctgaggaacg acgcgctgac caacgttgct gactcgaaag 1081 gctgctaccc gctgcacttg gcagcgtgga aaggagacgc ccagatagta cgtctgctca 1141 tccagcaagg gccctcacac accagagtca acgaacagaa tgctcttgag atcagagaac 1201 tcaaaaagta cggccccttt gacccttata tcaatgccaa gaacaacgac aatgagacgg 1261 ccctgcactg cgcggcccag tacggccaca cggaggtcgt gaaggctctg ctggaggagc 1321 tcacggaccc caccatgcgc aacaacaagt ttgaaacgcc cctggacttg gcggcgctgt 1381 acgggcggct ggaggtggtg aagctgctgc tgggtgcaca ccccaatctc ctgagctgca 1441 gcactcggaa acacacgccc ctgcacttgg cagcaaggaa tggccacaag gccgtggtgc 1501 aggtcctgct ggacgccggc atggatagca actatcagac ggagatgggc agtgctttgc 1561 atgaggctgc tttgtttggc aagaccgatg tggtgcaaat cctgctggct gcaggaatcg 1621 atgttaacat aaaggataac cgtggcctga ctgcccttga cactgtccgg gatctgccct 1681 ctcagaagag ccagcagata gccgccctga ttgaagatca catgaccgga aaaagaagcg 1741 tgaaagaagt ggacaggacc tccacagccc agctacctct cctctccaac acagacgcca 1801 tagcaccaat gtctcagggg agtatggaga agacagtgac tgagctgatc cttcattttg 1861 acacacatgc tgacgaggag ggcccctacg aggcactgta caacgctgtg tcctgccatt 1921 ccttggacag cacggccagt ggacgatcat cagacagaga ctccatgaac aaggaggccg 1981 aggccaccgg aacaagggct gctggagtga ggcctaggga acgtccgccg cctccagcaa 2041 aaccgccacc cgatgaagag gaggaagaac gcgtagataa gaagtatttt cccttggcag 2101 cctctgaggg cctggctgtg agacccagga ttcagagtag tgctccccag gaagaggagg 2161 agcacccata tgagctgctg ctcacggcag aaaccaagaa gctggggacc acagacggga 2221 ggactgaaga ccacaggcag agcggcagcg gccggagcca ggactctgtg gaggggcagg 2281 acgggcaggt cccagagcag ttctcaggtc tcctccacgg ctcctcccca gtgtgtgagg 2341 tggggcagga ccctttccag ctgctcacgg cccccagcca gagccacccg gagtcctccc 2401 agcaggacgc ctgccacgag gccagcatgc agctagagga gccaggcgtg caaggcactg 2461 aacccccgca gcccggcgtc cctgaccaaa gcaagagagt gggcttgccg gcaggcctga 2521 cagccctggc cagcagaaca tacctcgatg cattgactca cacagtacct ctgcgccctg 2581 ctggtgccga ggaagaagac cagagcgggc ccagaagcag agcccctccc accagcaagc 2641 ccaaagctga gctcaaactc agccgcagtt tgtctaagtc tgactctgac ctcctgacct 2701 gctcccccac ggaggacgcc acaatgggga gtcgcagcga gtccttgtcc aactgcagca 2761 tcgggaagaa gaggttggag aaatcacctt cctttgcctc agagtgggat gagattgaga 2821 aaatcatgag ctccattggg gaagggattg acttctctca ggagcagcag aagatctcag 2881 gttctcggac gctggagcag agcgtcgggg agtggctgga gtcgattggc ctgcagcagt 2941 acgagagcaa gctgctgctg aacggctttg atgatgtccg cttcctgggg tctaatgtga 3001 tggaagagca ggacctgagg gagattggca tcagtgaccc acagcaccgg cggaagctgc 3061 tgcaggctgc acgctccttg cccaaggtga aggcccttgg ctatgacggg gtcagcccca 3121 cctcagtgcc ctcatggctg gactcgctgg ggctgcagga ctatgtccac tctttcctgt 3181 ccagtggcta cagctccatc gacactgtga agaacctctg ggagctagag cttgtcaatg 3241 tcctgaaagt tcacctgctg ggccaccgca agcgcattat cgcctctcta gcagacaggc 3301 cctatgagga gccaccccag aagcctccaa ggttttccca gctaagatgc caggatttaa 3361 tctcccagac atcgtccccg ctgagccaga atgattcctg cacagggcgg tcggcagacc 3421 tgctgctgcc ctcagcagac acaagcagga ggcggcacga cagcctgccc gaccctggga 3481 ccgcctcccg agcagaccgc ttccgagtcc aggaggagcc cagtgagacc aagctgaccc 3541 tccgtccacc cagcctagcc gcgccctatg ctcctgttca gagctggcag caccagccag 3601 aaaagctcat ctttgagtcc tgcggttatg aggccaatta tctgggttcc atgctgatca 3661 aagatctgcg agggacagag tccacccaag atgcctgtgc caaaatgcgg aagtcaacgg 3721 agcacatgaa gaagatcccc accatcatcc tgtccatcac gtacaaaggt gtcaagttca 3781 tcgatgcttc caacaagaac gtcattgcgg agcacgagat ccggaacatt tcctgcgccg 3841 cgcaggaccc agaggacctg tgtaccttcg cgtacatcac caaggacctg cagacgagcc 3901 accactactg ccacgttttc agcacagtgg atgtgaacct gacctacgag atcatcctga 3961 ccctggggca ggcatttgag gtggcctatc agctggccct ccaagcccag aaatccagga 4021 ccatggcggc ctctgcagct tcgatgattg agacaaaatc ctccaaacca gtgcctaagc 4081 ctcgggtcgg catgaggaag tctgcagtgc cggtgccccc cgactctcgc tgttgtcact 4141 gtcacacctg caccactcac cgtccttcct acctaccgct gccgtccgtt agtcctggag 4201 tcaagctgga accacctgat tctgaccaag aggccccgtc ccacgctagt gtttcctgga 4261 tcgtggaccc aaagccagac tcgaagcgga gcctcagcac caagtacgag accactatct 4321 tcGGATCCGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG 4381 ATGACGATGA CAAGTAGTGA GGGCCCGATA TCTCGACAAT CAACCTCTGG ATTACAAAAT 4441 TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC 4501 TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT 4561 GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG 4621 CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG 4681 TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC 4741 CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT 4801 GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT 4861 GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG 4921 CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG 4981 GATCTCCCTT TGGGCCGCCT CCCCGCCTGG AAACGGGGGA GGCTAACTGA AACACGGAAG 5041 GAGACAATAC CGGAAGGAAC CCGCGCTATG ACGGCAATAA AAAGACAGAA TAAAACGCAC 5101 GGGTGTTGGG TCGTTTGTTC ATAAACGCGG GGTTCGGTCC CAGGGCTGGC ACTCTGTCGA 5161 TACCCCACCG AGACCCCATT GGGGCCAATA CGCCCGCGTT TCTTCCTTTT CCCCACCCCA 5221 CCCCCCAAGT TCGGGTGAAG GCCCAGGGCT CGCAGCCAAC GTCGGGGCGG CAGGCCCTGC 5281 CATAGCAGAT CTGCGCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC 5341 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT 5401 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG 5461 TCAAGCTCTA AATCGGGGCA TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA 5521 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT 5581 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG 5641 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGGGGATTTC 5701 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG 5761 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 5821 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 5881 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 5941 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 6001 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA 6061 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 6121 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 6181 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 6241 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 6301 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 6361 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 6421 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 6481 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 6541 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 6601 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 6661 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 6721 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 6781 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 6841 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 6901 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 6961 CTCTGGGGTT CGCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA 7021 TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG 7081 GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT 7141 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT 7201 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG 7261 TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG 7321 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 7381 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 7441 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 7501 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 7561 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 7621 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 7681 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 7741 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 7801 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 7861 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG 7921 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 7981 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 8041 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 8101 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG 8161 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 8221 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 8281 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 8341 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 8401 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 8461 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 8521 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 8581 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 8641 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 8701 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 8761 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 8821 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 8881 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 8941 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 9001 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 9061 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 9121 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 9181 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 9241 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 9301 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 9361 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 9421 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT 9481 CCCCTATGGT CGACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG 9541 CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA 9601 AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???